Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   O6P33_RS03710 Genome accession   NZ_CP114976
Coordinates   761477..762511 (+) Length   344 a.a.
NCBI ID   WP_269818903.1    Uniprot ID   -
Organism   Denitrificimonas caeni strain CE14     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 756477..767511
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O6P33_RS03680 (O6P33_03680) - 756991..757410 (-) 420 WP_269818896.1 DUF4426 domain-containing protein -
  O6P33_RS03685 (O6P33_03685) metW 757430..758032 (-) 603 WP_269818897.1 methionine biosynthesis protein MetW -
  O6P33_RS03690 (O6P33_03690) metX 758040..759179 (-) 1140 WP_269818898.1 homoserine O-succinyltransferase MetX -
  O6P33_RS03695 (O6P33_03695) - 759430..759732 (-) 303 WP_269818899.1 DUF167 family protein -
  O6P33_RS03700 (O6P33_03700) - 759729..760334 (-) 606 WP_269818901.1 YggT family protein -
  O6P33_RS03705 (O6P33_03705) proC 760344..761162 (-) 819 WP_269818902.1 pyrroline-5-carboxylate reductase -
  O6P33_RS03710 (O6P33_03710) pilT 761477..762511 (+) 1035 WP_269818903.1 type IV pilus twitching motility protein PilT Machinery gene
  O6P33_RS03715 (O6P33_03715) pilU 762551..763696 (+) 1146 WP_269818904.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  O6P33_RS03720 (O6P33_03720) - 763717..764409 (+) 693 WP_269818905.1 cytochrome b/b6 domain-containing protein -
  O6P33_RS03725 (O6P33_03725) - 764494..764961 (-) 468 WP_269818906.1 c-type cytochrome -
  O6P33_RS03730 (O6P33_03730) - 765502..766599 (+) 1098 WP_420094959.1 polyamine ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37895.47 Da        Isoelectric Point: 6.6656

>NTDB_id=767640 O6P33_RS03710 WP_269818903.1 761477..762511(+) (pilT) [Denitrificimonas caeni strain CE14]
MDITELLAFSAKQGASDLHLSSGLPPMIRVDGDVRRINLPAMDHKQVHALIYDIMNDKQRKDYEEFLETDFSFEVPGVAR
FRVNAFNQNRGAAAVFRTIPSKVLSMEDLGLGETFRNIADVPRGLVLVTGPTGSGKSTTLAALMDYINSSKYQHILTVED
PIEFVHESKKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMVRSMLSESLQAVISQTLLKKVGGGRVAAHEIMLGTPAIRNLIREDKVAQMYSAIQTGGALGMQTLDSCL
KGLLAKGLINRESAREKAKTPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=767640 O6P33_RS03710 WP_269818903.1 761477..762511(+) (pilT) [Denitrificimonas caeni strain CE14]
ATGGATATTACTGAACTGCTGGCATTTAGCGCTAAACAAGGTGCATCGGACCTGCATTTATCATCCGGCTTGCCGCCCAT
GATACGTGTCGATGGTGACGTGCGCCGAATTAATTTGCCGGCCATGGACCACAAGCAAGTGCATGCGCTGATCTACGATA
TTATGAATGACAAGCAGCGCAAGGATTATGAAGAGTTTTTAGAGACGGACTTTTCTTTTGAAGTGCCCGGTGTTGCGCGC
TTCCGGGTTAACGCTTTTAATCAAAACCGTGGTGCGGCGGCGGTATTTCGAACCATTCCTTCTAAAGTTTTAAGCATGGA
AGATCTTGGGTTGGGTGAAACCTTCCGTAATATTGCCGATGTGCCTCGCGGTTTGGTCCTGGTGACCGGTCCGACAGGTT
CGGGTAAGTCCACCACTTTGGCGGCGCTGATGGATTATATTAATAGCAGCAAATATCAGCATATCCTTACGGTGGAAGAC
CCAATTGAGTTCGTGCATGAGTCGAAAAAGTGCTTGATTAACCAACGTGAAGTGCACCGTGATACCCATGGTTTTAATGA
AGCGTTGCGTTCGGCGTTACGTGAAGACCCGGACATTATTCTGGTGGGTGAGTTGCGTGACTTGGAAACCATCCGCTTAG
CGCTGACCGCCGCGGAAACCGGTCACCTCGTCTTTGGCACTTTGCACACCACCTCAGCAGCTAAAACCATTGACCGGGTG
GTGGACGTGTTCCCCGCGGAAGAAAAATCCATGGTGCGTTCGATGCTGTCGGAATCCTTGCAGGCGGTTATTTCGCAAAC
CCTGTTAAAAAAGGTGGGTGGTGGCCGTGTGGCTGCACATGAAATCATGCTGGGTACTCCAGCAATCCGTAACCTGATTC
GTGAAGATAAAGTTGCGCAAATGTACTCTGCGATTCAAACCGGTGGTGCTTTGGGCATGCAGACTTTGGACAGTTGCTTA
AAAGGCTTATTAGCTAAAGGCTTAATCAACCGTGAAAGTGCGCGCGAGAAAGCCAAAACGCCAGAAAACTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

92.733

100

0.927
  pilT Pseudomonas aeruginosa PAK

89.535

100

0.895
  pilT Acinetobacter baumannii D1279779

84.302

100

0.843
  pilT Acinetobacter baumannii strain A118

84.302

100

0.843
  pilT Acinetobacter nosocomialis M2

84.012

100

0.84
  pilT Acinetobacter baylyi ADP1

83.14

100

0.831
  pilT Legionella pneumophila strain Lp02

76.627

98.256

0.753
  pilT Legionella pneumophila strain ERS1305867

76.627

98.256

0.753
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.733

97.965

0.683
  pilT Vibrio cholerae strain A1552

69.733

97.965

0.683
  pilT Neisseria meningitidis 8013

67.826

100

0.68
  pilT Neisseria gonorrhoeae MS11

67.536

100

0.677
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

100

0.517
  pilU Vibrio cholerae strain A1552

39.71

100

0.398
  pilU Acinetobacter baylyi ADP1

38.439

100

0.387
  pilU Pseudomonas stutzeri DSM 10701

39.104

97.384

0.381