Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   O5O48_RS06270 Genome accession   NZ_CP114836
Coordinates   1367097..1368131 (+) Length   344 a.a.
NCBI ID   WP_088276051.1    Uniprot ID   A0AAQ1G8D7
Organism   Halopseudomonas sp. MFKK-1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1362097..1373131
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O5O48_RS06235 - 1362274..1362705 (-) 432 WP_228247103.1 DUF4426 domain-containing protein -
  O5O48_RS06240 metW 1362728..1363318 (-) 591 WP_088276147.1 methionine biosynthesis protein MetW -
  O5O48_RS06245 - 1363321..1364463 (-) 1143 WP_228247102.1 homoserine O-acetyltransferase -
  O5O48_RS06250 - 1364590..1364880 (-) 291 WP_269688458.1 DUF167 family protein -
  O5O48_RS06255 - 1364886..1365476 (-) 591 WP_269688459.1 YggT family protein -
  O5O48_RS06260 proC 1365490..1366308 (-) 819 WP_269688460.1 pyrroline-5-carboxylate reductase -
  O5O48_RS06265 - 1366333..1367019 (-) 687 WP_269688461.1 YggS family pyridoxal phosphate-dependent enzyme -
  O5O48_RS06270 pilT 1367097..1368131 (+) 1035 WP_088276051.1 type IV pilus twitching motility protein PilT Machinery gene
  O5O48_RS06275 pilU 1368193..1369347 (+) 1155 WP_228247097.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  O5O48_RS06280 siaD 1369367..1370161 (-) 795 WP_228247096.1 biofilm regulation diguanylate cyclase SiaD -
  O5O48_RS06285 siaC 1370169..1370546 (-) 378 WP_228247095.1 biofilm regulation phosphoprotein SiaC -
  O5O48_RS06290 siaB 1370569..1371111 (-) 543 WP_228247094.1 biofilm regulation protein kinase SiaB -
  O5O48_RS06295 siaA 1371140..1373131 (-) 1992 WP_228247093.1 biofilm regulation protein phosphatase SiaA -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37810.68 Da        Isoelectric Point: 6.7714

>NTDB_id=767225 O5O48_RS06270 WP_088276051.1 1367097..1368131(+) (pilT) [Halopseudomonas sp. MFKK-1]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINVPAMEHKQVHALIYDIMNDKQRKDYEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGDVFKKISDVPRGLVLVTGPTGSGKSTSLAAMLDYINCNKYQHILTIED
PIEFVHESKKCLVNQREVHRDTLGFAEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
VDVFPGEEKAMVRSMLSESLQAVISQTLLKKIGGGRVAAHEIMIGTPAIRNLIREDKIAQMYSAIQTGGNLGMQTLDMCL
KSLVAKGLVSKESAREKAKVADGF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=767225 O5O48_RS06270 WP_088276051.1 1367097..1368131(+) (pilT) [Halopseudomonas sp. MFKK-1]
ATGGATATTACCGAGCTGTTAGCCTTCAGCGCTAAACAGGGCGCGTCGGACTTGCACCTGTCGGCAGGATTGCCGCCCAT
GATCCGTGTTGATGGCGACGTGCGCCGGATCAACGTACCGGCGATGGAGCACAAGCAGGTTCATGCGCTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGACTACGAGGAGTTCCTGGAGACCGACTTCTCGTTCGAGGTCCCCGGCGTAGCCCGG
TTCCGGGTTAACGCGTTCAACCAGAATCGTGGCGCAGGCGCGGTGTTCCGGACCATTCCTTCCAAGGTGCTGACCATGGA
AGATCTGGGCATGGGCGATGTGTTCAAGAAGATTTCCGATGTGCCGCGCGGGCTGGTGCTGGTGACCGGGCCGACCGGCT
CGGGCAAGTCCACCAGTCTGGCGGCCATGCTCGACTACATCAATTGCAACAAATACCAGCACATCCTGACCATCGAGGAC
CCGATCGAGTTCGTTCACGAGTCGAAGAAGTGTCTGGTCAACCAGCGTGAAGTGCACCGTGACACACTTGGCTTTGCCGA
AGCATTGCGCTCGGCACTGCGGGAAGACCCGGATATCATTCTGGTCGGCGAGATGCGCGACCTGGAAACCATTCGCCTGG
CGCTGACTGCGGCAGAAACCGGTCACCTGGTGTTCGGCACCCTGCATACCACCTCGGCGGCCAAGACCATCGACCGTATC
GTCGACGTGTTCCCCGGTGAGGAAAAGGCCATGGTGCGCTCGATGCTGTCCGAGTCACTGCAGGCGGTAATTTCCCAGAC
GCTGCTGAAGAAGATCGGCGGCGGCCGGGTCGCCGCGCACGAAATCATGATCGGCACACCGGCGATCCGGAACCTGATCC
GTGAAGACAAGATTGCGCAGATGTACTCGGCGATCCAGACCGGCGGCAACCTCGGCATGCAGACCCTCGACATGTGTTTG
AAGTCGCTGGTCGCCAAGGGCCTGGTCAGCAAGGAAAGCGCCCGTGAGAAAGCCAAGGTCGCCGACGGATTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

91.86

100

0.919
  pilT Pseudomonas stutzeri DSM 10701

90.988

100

0.91
  pilT Acinetobacter baumannii D1279779

84.012

100

0.84
  pilT Acinetobacter baumannii strain A118

84.012

100

0.84
  pilT Acinetobacter nosocomialis M2

83.721

100

0.837
  pilT Acinetobacter baylyi ADP1

81.395

100

0.814
  pilT Legionella pneumophila strain Lp02

75.148

98.256

0.738
  pilT Legionella pneumophila strain ERS1305867

75.148

98.256

0.738
  pilT Neisseria meningitidis 8013

67.826

100

0.68
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.436

97.965

0.68
  pilT Vibrio cholerae strain A1552

69.436

97.965

0.68
  pilT Neisseria gonorrhoeae MS11

67.536

100

0.677
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.917

98.547

0.512
  pilU Pseudomonas stutzeri DSM 10701

41.791

97.384

0.407
  pilU Vibrio cholerae strain A1552

40.233

99.709

0.401
  pilU Acinetobacter baylyi ADP1

39.412

98.837

0.39