Detailed information NTG0767226

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: O5O48_RS06275
• Genome accession: NZ_CP114836
• Coordinates: 1368193..1369347 (+)
• Length: 384 a.a.
• NCBI ID: WP_228247097.1
• Uniprot ID: -
• Organism: Halopseudomonas sp. MFKK-1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1363193..1374347

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
O5O48_RS06245	-	1363321..1364463 (-)	1143	WP_228247102.1	homoserine O-acetyltransferase	-
O5O48_RS06250	-	1364590..1364880 (-)	291	WP_269688458.1	DUF167 family protein	-
O5O48_RS06255	-	1364886..1365476 (-)	591	WP_269688459.1	YggT family protein	-
O5O48_RS06260	proC	1365490..1366308 (-)	819	WP_269688460.1	pyrroline-5-carboxylate reductase	-
O5O48_RS06265	-	1366333..1367019 (-)	687	WP_269688461.1	YggS family pyridoxal phosphate-dependent enzyme	-
O5O48_RS06270	pilT	1367097..1368131 (+)	1035	WP_088276051.1	type IV pilus twitching motility protein PilT	Machinery gene
O5O48_RS06275	pilU	1368193..1369347 (+)	1155	WP_228247097.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
O5O48_RS06280	siaD	1369367..1370161 (-)	795	WP_228247096.1	biofilm regulation diguanylate cyclase SiaD	-
O5O48_RS06285	siaC	1370169..1370546 (-)	378	WP_228247095.1	biofilm regulation phosphoprotein SiaC	-
O5O48_RS06290	siaB	1370569..1371111 (-)	543	WP_228247094.1	biofilm regulation protein kinase SiaB	-
O5O48_RS06295	siaA	1371140..1373131 (-)	1992	WP_228247093.1	biofilm regulation protein phosphatase SiaA	-

Sequence

Protein

• Length: 384 a.a.
• Molecular weight: 43008.57 Da
• Isoelectric Point: 6.6936

>NTDB_id=767226 O5O48_RS06275 WP_228247097.1 1368193..1369347(+) (pilU) [Halopseudomonas sp. MFKK-1]
MEFEKLLRLMVEKGASDLFITAGVPPSMKVNGKILPVTKTPLSPEQTRETVLGVMTEAQRREFAEKRECNFAISARGIGR
FRVSSFYQRNLVGMVLRRIEVNIPTMEELRLPEVLKQLAMTKRGLVIFVGATGTGKSTSLASMIGYRNKHSSGHIISIED
PIEFIHQHQNCIVTQREVGIDTDSFEVALKNTLRQAPDVILIGEVRTKETMDHAVAFAETGHLCLATLHANNANQALDRI
IHFFPTEMHQQVWMDLSLNLKAIVAQQLIPTPDGKGRRAAIEVLLNTPLAADMIRKGEVHELKPLMARSNELGMQTFDQA
LYKLYSAGEITYEDALLHADSANDLRLMIKLGSETDGQHLMQDQLSHGLSLEEEEEVASRTRRF

Nucleotide

• Length: 1155 bp

>NTDB_id=767226 O5O48_RS06275 WP_228247097.1 1368193..1369347(+) (pilU) [Halopseudomonas sp. MFKK-1]
ATGGAGTTCGAGAAGCTGTTGCGGTTGATGGTCGAGAAGGGTGCGTCCGACCTGTTCATTACCGCCGGTGTGCCACCGAG
CATGAAGGTGAATGGCAAGATCCTGCCGGTTACCAAAACCCCGCTGTCGCCGGAGCAGACCCGCGAAACCGTGCTGGGCG
TGATGACCGAAGCGCAGCGTCGCGAGTTCGCCGAGAAGCGCGAATGCAACTTCGCCATCAGTGCGCGCGGCATTGGCCGG
TTCCGGGTCAGCTCGTTCTATCAGCGCAATCTGGTGGGCATGGTGTTGCGCCGTATCGAGGTCAACATTCCGACCATGGA
AGAACTGCGGCTGCCGGAGGTGCTGAAGCAGCTGGCCATGACCAAGCGCGGTCTGGTGATCTTCGTCGGCGCTACCGGTA
CCGGCAAGTCGACCTCGCTGGCCTCAATGATCGGCTACCGCAACAAGCACTCCAGCGGGCACATCATCTCCATCGAAGAT
CCGATCGAATTCATTCACCAGCATCAGAACTGCATTGTTACCCAGCGCGAGGTCGGTATTGATACCGACTCGTTCGAGGT
GGCGCTGAAGAACACCTTGCGTCAGGCGCCGGACGTGATTCTGATCGGTGAGGTGCGGACCAAGGAAACCATGGATCACG
CGGTGGCCTTTGCCGAAACCGGCCACCTGTGTCTGGCTACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATTCACTTCTTCCCGACCGAGATGCATCAGCAGGTCTGGATGGACCTGTCACTGAATCTCAAGGCGATTGTTGCCCAGCA
GCTGATTCCCACGCCCGATGGCAAGGGCCGTCGGGCTGCCATCGAAGTGCTGCTTAATACGCCGCTGGCCGCCGACATGA
TTCGCAAGGGTGAGGTGCATGAGCTCAAGCCGTTAATGGCCCGCTCCAATGAATTGGGCATGCAGACCTTCGATCAGGCG
CTCTACAAGCTCTACAGTGCCGGCGAGATCACCTATGAAGATGCGCTGTTGCACGCCGACTCGGCGAACGACCTGCGTCT
GATGATCAAGCTGGGCTCGGAAACCGACGGTCAGCACCTGATGCAGGACCAGCTGAGCCACGGCCTGTCGCTGGAAGAAG
AGGAAGAAGTCGCCTCGCGCACGCGGCGGTTCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	84.856	99.74	0.846
pilU	Acinetobacter baylyi ADP1	66.387	92.969	0.617
pilU	Vibrio cholerae strain A1552	60	91.146	0.547
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	44.543	88.281	0.393