Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   N7E60_RS11990 Genome accession   NZ_CP114584
Coordinates   2557137..2558162 (-) Length   341 a.a.
NCBI ID   WP_069591174.1    Uniprot ID   A0A2A4GI28
Organism   Salinivibrio proteolyticus strain TGB10     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2552137..2563162
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N7E60_RS11960 (N7E60_11960) - 2552616..2553119 (+) 504 WP_269597580.1 SprT family zinc-dependent metalloprotease -
  N7E60_RS11965 (N7E60_11965) rsmE 2553234..2553965 (+) 732 WP_069591180.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  N7E60_RS11970 (N7E60_11970) gshB 2553987..2554931 (+) 945 WP_167314822.1 glutathione synthase -
  N7E60_RS11975 (N7E60_11975) - 2555022..2555588 (+) 567 WP_069591178.1 YqgE/AlgH family protein -
  N7E60_RS11980 (N7E60_11980) ruvX 2555585..2556013 (+) 429 WP_069591176.1 Holliday junction resolvase RuvX -
  N7E60_RS11985 (N7E60_11985) pilU 2556010..2557128 (-) 1119 WP_269597582.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  N7E60_RS11990 (N7E60_11990) pilT 2557137..2558162 (-) 1026 WP_069591174.1 type IV pilus twitching motility protein PilT Machinery gene
  N7E60_RS11995 (N7E60_11995) - 2558186..2558887 (+) 702 WP_069591173.1 YggS family pyridoxal phosphate-dependent enzyme -
  N7E60_RS12000 (N7E60_12000) proC 2558972..2559790 (+) 819 WP_077457418.1 pyrroline-5-carboxylate reductase -
  N7E60_RS12005 (N7E60_12005) - 2559807..2560361 (+) 555 WP_069591171.1 YggT family protein -
  N7E60_RS12010 (N7E60_12010) - 2560361..2560657 (+) 297 WP_069591169.1 DUF167 family protein -
  N7E60_RS12015 (N7E60_12015) - 2560719..2561159 (+) 441 WP_069591168.1 DUF4426 domain-containing protein -
  N7E60_RS12020 (N7E60_12020) rdgB 2561188..2561784 (+) 597 WP_096629937.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  N7E60_RS12025 (N7E60_12025) hemW 2561787..2562944 (+) 1158 WP_269597585.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 341 a.a.        Molecular weight: 37332.72 Da        Isoelectric Point: 6.5569

>NTDB_id=766606 N7E60_RS11990 WP_069591174.1 2557137..2558162(-) (pilT) [Salinivibrio proteolyticus strain TGB10]
MDITELLAFSVKHNASDLHLSAGVAPMVRVDGDVRKLSLPALSHQEVNQLVLDVMNDNQQREFQAQLEVDFSFHMQSVGR
FRVNAFHQQHGCAAVFRVIPETVPSLADLHAPEIFADFARLSNGLVLVTGPTGSGKSTTLAAMVDHINRHAPKHILTIED
PIEFIHHPQQSLINQREVKRDTLSFQQALRSALREDPDVILVGELRDLDTISLALTAAETGHLVFGTLHTSSAAKTVDRI
IDVFPGSDKPLVRSMLSASLRAVVSQQLIKTAQGGRVAAHEIMIASPAIRNLIREDKVAQMVSMIQTGSNQGMQTMEQAL
ADLVGQGTIAPEQRDRVLRAL

Nucleotide


Download         Length: 1026 bp        

>NTDB_id=766606 N7E60_RS11990 WP_069591174.1 2557137..2558162(-) (pilT) [Salinivibrio proteolyticus strain TGB10]
ATGGATATCACTGAGCTATTGGCCTTTAGTGTAAAACATAATGCGTCAGATCTACATCTTTCCGCGGGAGTGGCGCCTAT
GGTGCGGGTAGATGGCGATGTGCGCAAGTTGAGTTTGCCAGCGTTGAGCCATCAAGAGGTCAATCAACTGGTCTTGGATG
TGATGAATGATAACCAGCAGCGTGAATTTCAAGCGCAGTTGGAAGTGGATTTTTCATTTCATATGCAGTCTGTGGGACGT
TTTCGGGTTAATGCATTTCATCAGCAACACGGCTGCGCGGCGGTATTTCGGGTGATCCCCGAGACAGTCCCCAGTTTGGC
GGATCTCCATGCCCCCGAGATTTTTGCGGATTTTGCTCGGCTCTCAAATGGATTAGTGTTGGTCACCGGCCCGACGGGCT
CGGGGAAGTCCACCACATTGGCGGCCATGGTTGATCATATTAACCGCCACGCACCTAAGCATATTCTCACCATCGAAGAC
CCGATTGAGTTTATCCATCACCCACAACAAAGTTTGATTAATCAGCGTGAAGTGAAGCGCGATACCTTATCGTTTCAGCA
AGCGCTGCGTTCGGCGTTGCGTGAAGATCCCGATGTGATTTTGGTAGGAGAGCTGCGAGATCTCGATACCATTAGTTTGG
CGTTAACGGCGGCGGAAACTGGACATCTGGTATTCGGCACCTTGCATACCTCAAGCGCAGCAAAAACAGTCGACCGTATT
ATTGATGTCTTTCCCGGCAGTGATAAGCCGCTGGTCCGCTCGATGCTTTCGGCCTCACTGCGGGCGGTTGTGTCTCAGCA
GCTGATAAAAACAGCGCAAGGTGGGCGCGTGGCCGCGCATGAAATCATGATCGCAAGCCCAGCGATTCGTAACCTCATCC
GTGAGGATAAGGTAGCGCAAATGGTGTCGATGATTCAAACCGGCTCCAATCAAGGGATGCAAACCATGGAGCAAGCACTG
GCCGATTTAGTCGGGCAAGGGACGATCGCGCCGGAACAACGCGATCGCGTATTACGTGCGCTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2A4GI28

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.171

97.654

0.695
  pilT Vibrio cholerae strain A1552

71.171

97.654

0.695
  pilT Acinetobacter baumannii D1279779

66.566

97.361

0.648
  pilT Acinetobacter baumannii strain A118

66.566

97.361

0.648
  pilT Acinetobacter nosocomialis M2

66.566

97.361

0.648
  pilT Neisseria meningitidis 8013

65.766

97.654

0.642
  pilT Neisseria gonorrhoeae MS11

64.985

98.827

0.642
  pilT Acinetobacter baylyi ADP1

66.565

96.481

0.642
  pilT Pseudomonas aeruginosa PAK

65.465

97.654

0.639
  pilT Pseudomonas stutzeri DSM 10701

65.455

96.774

0.633
  pilT Legionella pneumophila strain Lp02

64.242

96.774

0.622
  pilT Legionella pneumophila strain ERS1305867

64.242

96.774

0.622
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.778

95.015

0.501
  pilU Pseudomonas stutzeri DSM 10701

41.742

97.654

0.408
  pilU Acinetobacter baylyi ADP1

41.566

97.361

0.405
  pilU Vibrio cholerae strain A1552

40.615

95.308

0.387