Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   N7E60_RS11985 Genome accession   NZ_CP114584
Coordinates   2556010..2557128 (-) Length   372 a.a.
NCBI ID   WP_269597582.1    Uniprot ID   -
Organism   Salinivibrio proteolyticus strain TGB10     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2551010..2562128
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N7E60_RS11955 (N7E60_11955) metK 2551318..2552472 (+) 1155 WP_069591183.1 methionine adenosyltransferase -
  N7E60_RS11960 (N7E60_11960) - 2552616..2553119 (+) 504 WP_269597580.1 SprT family zinc-dependent metalloprotease -
  N7E60_RS11965 (N7E60_11965) rsmE 2553234..2553965 (+) 732 WP_069591180.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  N7E60_RS11970 (N7E60_11970) gshB 2553987..2554931 (+) 945 WP_167314822.1 glutathione synthase -
  N7E60_RS11975 (N7E60_11975) - 2555022..2555588 (+) 567 WP_069591178.1 YqgE/AlgH family protein -
  N7E60_RS11980 (N7E60_11980) ruvX 2555585..2556013 (+) 429 WP_069591176.1 Holliday junction resolvase RuvX -
  N7E60_RS11985 (N7E60_11985) pilU 2556010..2557128 (-) 1119 WP_269597582.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  N7E60_RS11990 (N7E60_11990) pilT 2557137..2558162 (-) 1026 WP_069591174.1 type IV pilus twitching motility protein PilT Machinery gene
  N7E60_RS11995 (N7E60_11995) - 2558186..2558887 (+) 702 WP_069591173.1 YggS family pyridoxal phosphate-dependent enzyme -
  N7E60_RS12000 (N7E60_12000) proC 2558972..2559790 (+) 819 WP_077457418.1 pyrroline-5-carboxylate reductase -
  N7E60_RS12005 (N7E60_12005) - 2559807..2560361 (+) 555 WP_069591171.1 YggT family protein -
  N7E60_RS12010 (N7E60_12010) - 2560361..2560657 (+) 297 WP_069591169.1 DUF167 family protein -
  N7E60_RS12015 (N7E60_12015) - 2560719..2561159 (+) 441 WP_069591168.1 DUF4426 domain-containing protein -
  N7E60_RS12020 (N7E60_12020) rdgB 2561188..2561784 (+) 597 WP_096629937.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41089.30 Da        Isoelectric Point: 6.9093

>NTDB_id=766605 N7E60_RS11985 WP_269597582.1 2556010..2557128(-) (pilU) [Salinivibrio proteolyticus strain TGB10]
MKLSSVLVAMQEHTASDAYISVDAPVMLRVDGRLKPLSSPLSLAAINVMLNSAMNEEQKQQFITTREANFAVKRSVGRFR
ISAYWQRDMPSMVIRRIETEIPRLPSLGTPVVLEELAMQKRGLVLVVGATGSGKSTTMAAMMGFRNHYGQGHILTVEDPI
EFVHEHQQCIVSQREIGIDTQDYETALKNALRQAPDMVVIGEIRTPETMNFALQFAETGHLCLATLHANNAYQALERVLH
LMPKAQRETFLFDLSMNLKGVIAQQLVPGVNGVGRHGAFELLINNAHVAELIRNGELHALKKAMLSGEHFGMQTFDQSLY
ALYVQGSISEDEALRHADSANELKLMIKTGAKHSAPGSALDHVRIERAGSLD

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=766605 N7E60_RS11985 WP_269597582.1 2556010..2557128(-) (pilU) [Salinivibrio proteolyticus strain TGB10]
ATGAAACTTAGTAGTGTGCTGGTGGCGATGCAAGAGCACACCGCCTCGGATGCGTATATTTCTGTGGATGCGCCTGTGAT
GTTACGGGTGGATGGACGCTTAAAACCTCTGTCTTCGCCGTTGTCGCTGGCGGCAATCAACGTCATGTTGAACAGTGCGA
TGAACGAGGAACAAAAGCAGCAATTTATTACCACGCGGGAAGCGAACTTTGCGGTAAAACGCAGTGTTGGGCGTTTTCGG
ATCAGTGCTTATTGGCAGCGTGATATGCCCAGTATGGTGATCCGCCGCATAGAAACCGAGATTCCGCGTTTGCCATCGTT
AGGCACCCCAGTGGTGCTCGAAGAGCTTGCGATGCAAAAGCGAGGCTTGGTCTTAGTGGTTGGGGCGACGGGGTCGGGTA
AGTCGACAACCATGGCGGCGATGATGGGGTTTCGTAATCACTATGGGCAAGGCCATATCCTAACGGTTGAAGATCCGATT
GAATTTGTTCATGAGCATCAGCAATGCATTGTCAGCCAACGCGAAATTGGTATCGATACCCAAGATTATGAAACAGCGCT
AAAAAATGCGCTCAGGCAAGCGCCAGATATGGTGGTAATTGGTGAAATACGAACGCCAGAAACCATGAACTTCGCGCTGC
AGTTTGCGGAAACGGGCCATCTTTGCTTGGCCACCTTACATGCGAATAACGCTTATCAAGCACTGGAGCGGGTATTACAC
TTGATGCCTAAAGCACAGCGAGAGACCTTTTTGTTTGATTTGTCGATGAACTTAAAAGGTGTTATCGCTCAACAGTTGGT
ACCAGGTGTGAATGGGGTTGGGCGGCATGGTGCCTTTGAATTATTGATCAATAACGCGCATGTGGCAGAGCTGATCCGTA
ATGGTGAACTACACGCGTTGAAAAAAGCCATGTTGAGCGGGGAGCATTTTGGTATGCAGACCTTTGATCAATCGCTGTAT
GCGCTTTATGTGCAAGGGAGTATCAGCGAGGATGAGGCGCTGCGACATGCGGACTCGGCCAATGAATTGAAGTTGATGAT
CAAAACCGGTGCTAAGCATAGTGCGCCGGGCAGCGCACTGGATCATGTACGGATTGAAAGAGCGGGCTCGCTGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(29-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.929

98.387

0.599
  pilU Pseudomonas stutzeri DSM 10701

53.107

95.161

0.505
  pilU Acinetobacter baylyi ADP1

49.304

96.505

0.476