Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   O1449_RS03710 Genome accession   NZ_CP114264
Coordinates   772413..773450 (-) Length   345 a.a.
NCBI ID   WP_087544931.1    Uniprot ID   A0A9E9L6K3
Organism   Acinetobacter sp. TR3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 767413..778450
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O1449_RS03680 (O1449_03685) - 768063..768518 (+) 456 WP_269229781.1 bacteriohemerythrin -
  O1449_RS03685 (O1449_03690) - 768767..769849 (+) 1083 WP_269239198.1 hypothetical protein -
  O1449_RS03690 (O1449_03695) - 769849..770169 (+) 321 WP_269239199.1 RnfH family protein -
  O1449_RS03695 (O1449_03700) bamE 770228..770626 (-) 399 WP_269229784.1 outer membrane protein assembly factor BamE -
  O1449_RS03700 (O1449_03705) fur 770738..771175 (+) 438 WP_004663428.1 ferric iron uptake transcriptional regulator -
  O1449_RS03705 (O1449_03710) pilU 771270..772388 (-) 1119 WP_005158228.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  O1449_RS03710 (O1449_03715) pilT 772413..773450 (-) 1038 WP_087544931.1 type IV pilus twitching motility protein PilT Machinery gene
  O1449_RS03715 (O1449_03720) - 773574..774266 (+) 693 WP_269239200.1 YggS family pyridoxal phosphate-dependent enzyme -
  O1449_RS03720 (O1449_03725) - 774450..775172 (+) 723 WP_269239201.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38356.96 Da        Isoelectric Point: 6.3478

>NTDB_id=765292 O1449_RS03710 WP_087544931.1 772413..773450(-) (pilT) [Acinetobacter sp. TR3]
MDITELLAFAVKNGASDLHLSSGMPPMIRVDGEVRRINLPALEHKDVHRLVYDIMNDKQRRDYEEDLETDFSFEVPNIAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGSIFKEICDYPRGIVLVTGPTGSGKSTTLAAMIDYINENRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLVRENKVAQMYSAIQTGANYGMTTLDQSL
KTLVSKGVISPQVARTVAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=765292 O1449_RS03710 WP_087544931.1 772413..773450(-) (pilT) [Acinetobacter sp. TR3]
ATGGATATTACAGAATTACTAGCATTTGCAGTTAAAAACGGTGCTTCAGACTTACATCTTTCATCAGGTATGCCACCAAT
GATTCGTGTAGATGGTGAAGTCCGTCGTATTAATTTACCAGCACTAGAACATAAAGATGTACACCGCTTAGTGTATGACA
TCATGAATGATAAACAACGTCGTGATTATGAAGAAGATCTAGAAACTGACTTTTCATTTGAAGTGCCTAATATTGCACGT
TTCCGTGTGAACGCATTTAACCAAAACCGTGGCGCTGGCGCGGTATTCCGTACCATTCCATCTAAAGTTTTAACCATGGA
AGACCTTGGTTTAGGTTCAATTTTTAAAGAAATTTGTGATTATCCACGGGGTATCGTTTTGGTCACAGGACCTACGGGTT
CTGGTAAATCAACGACGCTCGCTGCGATGATCGACTATATCAACGAAAACCGCTATGACCACATTTTAACGGTTGAAGAC
CCAATCGAATTTGTACACCAATCTAAAAAATGCCTGATCAACCAGCGTGAAGTGCACCGTGATACACATGGCTTCAATGA
AGCACTGCGTTCAGCACTGCGTGAAGACCCTGACATTATCCTTGTGGGTGAGATGCGTGATTTGGAAACCATTCGTCTGG
CATTGACTGCTGCGGAAACGGGTCACTTGGTTTTTGGTACACTGCATACGACCTCTGCAGCGAAAACAATTGACCGTGTC
ATCGACGTATTCCCTGCCGAAGAAAAAGATATGGTTCGTGCCATGCTTTCTGAATCACTACAAGCCGTTATTTCACAAAC
ATTATTGAAGAAAAATGGCGGTGGTCGTGTTGCAGCACATGAAATCATGATTGGAATTCCAGCAATTCGTAACCTTGTAC
GTGAAAATAAAGTAGCTCAGATGTATTCTGCCATCCAAACGGGTGCAAACTACGGTATGACTACACTTGATCAAAGTCTT
AAGACCTTGGTTTCAAAAGGTGTTATTAGTCCTCAAGTTGCCCGTACCGTAGCAAAACAACCTGAGTCATTCCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

96.232

100

0.962
  pilT Acinetobacter baumannii D1279779

95.942

100

0.959
  pilT Acinetobacter baumannii strain A118

95.942

100

0.959
  pilT Acinetobacter baylyi ADP1

86.919

99.71

0.867
  pilT Pseudomonas stutzeri DSM 10701

82.849

99.71

0.826
  pilT Pseudomonas aeruginosa PAK

82.558

99.71

0.823
  pilT Legionella pneumophila strain Lp02

75

99.71

0.748
  pilT Legionella pneumophila strain ERS1305867

75

99.71

0.748
  pilT Neisseria meningitidis 8013

68.696

100

0.687
  pilT Neisseria gonorrhoeae MS11

68.116

100

0.681
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.181

96.232

0.675
  pilT Vibrio cholerae strain A1552

70.181

96.232

0.675
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.725

100

0.507
  pilU Vibrio cholerae strain A1552

41.493

97.101

0.403
  pilU Pseudomonas stutzeri DSM 10701

40.785

95.942

0.391
  pilU Acinetobacter baylyi ADP1

38.728

100

0.388