Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   O1449_RS03705 Genome accession   NZ_CP114264
Coordinates   771270..772388 (-) Length   372 a.a.
NCBI ID   WP_005158228.1    Uniprot ID   N9LTK6
Organism   Acinetobacter sp. TR3     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 766270..777388
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O1449_RS03675 (O1449_03680) - 766938..767786 (+) 849 WP_269229780.1 class II glutamine amidotransferase -
  O1449_RS03680 (O1449_03685) - 768063..768518 (+) 456 WP_269229781.1 bacteriohemerythrin -
  O1449_RS03685 (O1449_03690) - 768767..769849 (+) 1083 WP_269239198.1 hypothetical protein -
  O1449_RS03690 (O1449_03695) - 769849..770169 (+) 321 WP_269239199.1 RnfH family protein -
  O1449_RS03695 (O1449_03700) bamE 770228..770626 (-) 399 WP_269229784.1 outer membrane protein assembly factor BamE -
  O1449_RS03700 (O1449_03705) fur 770738..771175 (+) 438 WP_004663428.1 ferric iron uptake transcriptional regulator -
  O1449_RS03705 (O1449_03710) pilU 771270..772388 (-) 1119 WP_005158228.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  O1449_RS03710 (O1449_03715) pilT 772413..773450 (-) 1038 WP_087544931.1 type IV pilus twitching motility protein PilT Machinery gene
  O1449_RS03715 (O1449_03720) - 773574..774266 (+) 693 WP_269239200.1 YggS family pyridoxal phosphate-dependent enzyme -
  O1449_RS03720 (O1449_03725) - 774450..775172 (+) 723 WP_269239201.1 hypothetical protein -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41641.80 Da        Isoelectric Point: 6.4346

>NTDB_id=765291 O1449_RS03705 WP_005158228.1 771270..772388(-) (pilU) [Acinetobacter sp. TR3]
MDFNDLLNLMIQQSASDLFVTADVEPSMKINGQIVPVSKSKLSGEVVGQLLHSIMSDKQRKEFAETRECNFAIVNREKTA
RFRVSAFQQRDMPGMVLRRIETVIPTMDELKLPSVLKELAMTKRGIIIFVGATGTGKSTSLASMIGYRNQNSKGHIITIE
DPIEFVHQHAGCIVTQREVGIDTDSFEVALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVLATLHANNANQALDR
IVHFFEADRHPQLHMDLSLNLKAMVAQQLIPTPDGNSRRAAIEILINTPLLADYIRKGEVHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGEITYKDALKHADSPNDLRLTIKLSEEGTDQLLSASRNITFDGQ

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=765291 O1449_RS03705 WP_005158228.1 771270..772388(-) (pilU) [Acinetobacter sp. TR3]
ATGGACTTTAATGATTTACTCAATTTAATGATTCAACAAAGTGCATCTGATTTATTTGTCACTGCAGATGTTGAACCATC
AATGAAGATCAACGGTCAAATTGTGCCTGTTTCAAAGTCTAAACTCTCAGGTGAAGTCGTTGGTCAGTTACTTCATTCAA
TTATGTCAGATAAGCAGCGTAAAGAATTTGCTGAAACAAGAGAGTGTAATTTTGCGATCGTCAATCGTGAGAAAACAGCC
CGTTTCCGTGTAAGCGCCTTTCAACAACGTGATATGCCTGGAATGGTTTTACGTCGTATTGAAACTGTTATTCCAACCAT
GGATGAACTCAAGCTCCCTAGCGTGCTGAAAGAACTCGCCATGACCAAACGCGGTATCATTATCTTTGTAGGCGCAACAG
GTACAGGTAAATCAACCTCATTAGCTTCGATGATTGGTTACCGTAACCAAAATTCAAAGGGTCATATTATTACCATTGAA
GACCCAATTGAATTCGTACATCAACATGCGGGTTGTATTGTCACTCAACGTGAAGTCGGGATTGATACAGATTCATTTGA
AGTCGCATTAAAAAATACGCTTCGCCAAGCACCTGATGTGATCTTGATTGGTGAGATTCGTTCTCGAGAAACCATGGACT
ATGCGATTGCTTTTGCGGAAACAGGTCACTTAGTACTTGCAACACTACACGCAAATAATGCGAACCAAGCACTAGATCGC
ATTGTCCACTTCTTTGAAGCAGATCGTCATCCTCAATTGCATATGGATTTATCACTTAATCTCAAGGCGATGGTTGCTCA
ACAGTTGATCCCAACGCCAGATGGTAATTCACGTCGCGCAGCAATTGAGATTCTGATTAATACACCACTTTTGGCAGACT
ACATACGCAAAGGTGAAGTACACGAAATCAAAGATCTCATGAAGCGTTCTCGAGAATTGGGGATGCAGACTTTTGACCAA
GCATTATTTGATTTATACAAAGCAGGTGAAATTACGTATAAAGATGCACTGAAGCATGCTGACTCGCCAAATGACTTACG
TCTCACAATTAAACTTTCAGAAGAAGGTACAGATCAGTTATTGAGTGCGAGCCGAAATATTACCTTTGATGGTCAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9LTK6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

75.397

100

0.766
  pilU Pseudomonas stutzeri DSM 10701

71.159

99.731

0.71
  pilU Vibrio cholerae strain A1552

55.676

99.462

0.554
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.484

97.849

0.406
  pilT Pseudomonas aeruginosa PAK

40.462

93.011

0.376
  pilT Neisseria gonorrhoeae MS11

40.294

91.398

0.368
  pilT Neisseria meningitidis 8013

40.058

91.935

0.368
  pilT Pseudomonas stutzeri DSM 10701

39.306

93.011

0.366
  pilT Legionella pneumophila strain ERS1305867

40.979

87.903

0.36
  pilT Legionella pneumophila strain Lp02

40.979

87.903

0.36
  pilT Acinetobacter baumannii D1279779

39.067

92.204

0.36
  pilT Acinetobacter nosocomialis M2

39.067

92.204

0.36
  pilT Acinetobacter baumannii strain A118

39.067

92.204

0.36