Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OX890_RS16410 Genome accession   NZ_CP113808
Coordinates   3884992..3886029 (-) Length   345 a.a.
NCBI ID   WP_006082517.1    Uniprot ID   A0AA50Q7P7
Organism   Shewanella sp. DAU305     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3879992..3891029
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OX890_RS16375 (OX890_16375) - 3880101..3880418 (+) 318 WP_268649591.1 hypothetical protein -
  OX890_RS16380 (OX890_16380) yciH 3880597..3880926 (+) 330 WP_219253466.1 stress response translation initiation inhibitor YciH -
  OX890_RS16385 (OX890_16385) - 3880993..3881556 (+) 564 WP_006082512.1 YqgE/AlgH family protein -
  OX890_RS16390 (OX890_16390) ruvX 3881586..3882011 (+) 426 WP_006082513.1 Holliday junction resolvase RuvX -
  OX890_RS16395 (OX890_16395) hemH 3882097..3883110 (-) 1014 WP_268649592.1 ferrochelatase -
  OX890_RS16400 (OX890_16400) - 3883143..3883682 (-) 540 WP_037392614.1 glutathione peroxidase -
  OX890_RS16405 (OX890_16405) pilU 3883870..3884982 (-) 1113 WP_106649931.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OX890_RS16410 (OX890_16410) pilT 3884992..3886029 (-) 1038 WP_006082517.1 type IV pilus twitching motility protein PilT Machinery gene
  OX890_RS16415 (OX890_16415) - 3886065..3886763 (+) 699 WP_268649593.1 YggS family pyridoxal phosphate-dependent enzyme -
  OX890_RS16420 (OX890_16420) proC 3886979..3887797 (+) 819 WP_006082519.1 pyrroline-5-carboxylate reductase -
  OX890_RS16425 (OX890_16425) - 3887846..3888394 (+) 549 WP_006082520.1 YggT family protein -
  OX890_RS16430 (OX890_16430) yggU 3888394..3888693 (+) 300 WP_012587167.1 DUF167 family protein YggU -
  OX890_RS16435 (OX890_16435) - 3888825..3889259 (+) 435 WP_006082522.1 DUF4426 domain-containing protein -
  OX890_RS16440 (OX890_16440) rdgB 3889399..3890016 (+) 618 WP_012089754.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38297.94 Da        Isoelectric Point: 6.9095

>NTDB_id=763171 OX890_RS16410 WP_006082517.1 3884992..3886029(-) (pilT) [Shewanella sp. DAU305]
MEITELLAFSVKHKASDLHLSAGISPMIRVDGEVRKINLPALDHQGVHSLVYDIMNDKQRKDYEEHLEIDFSFEVPNLAR
FRVNAFNQSRGAAAVFRTIPSDILSLEQLGAPEIFKKIASFPRGLVLVTGPTGSGKSTTLAAMVDYVNENRHDHILTIED
PIEFVHQNKQCLINQREVHRHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAGEKDMVRTMLSESLQAVISQTLIKKVGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMAHGMQTLEQCL
QNLVNRGLITREDAMAKSSNKQATF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=763171 OX890_RS16410 WP_006082517.1 3884992..3886029(-) (pilT) [Shewanella sp. DAU305]
ATGGAAATCACTGAGTTATTGGCATTTAGTGTAAAACACAAAGCCTCGGATCTACACCTTTCTGCAGGGATATCTCCCAT
GATCCGTGTCGACGGTGAAGTGAGAAAGATTAACTTGCCCGCGCTCGATCACCAAGGCGTACACAGCCTAGTGTACGACA
TAATGAATGATAAGCAGCGCAAGGACTACGAAGAACATTTAGAAATCGATTTCTCGTTCGAAGTCCCTAATCTTGCACGC
TTTCGTGTTAACGCATTCAATCAATCCCGTGGTGCTGCGGCCGTATTTCGTACCATTCCTAGCGACATCTTGTCACTTGA
GCAATTAGGTGCGCCGGAGATTTTTAAAAAAATCGCCAGTTTCCCTCGGGGATTAGTCCTAGTGACTGGGCCAACAGGTT
CGGGTAAGAGTACCACACTTGCCGCTATGGTGGATTACGTTAACGAAAATCGACACGATCACATCTTAACCATTGAAGAT
CCTATCGAATTCGTACACCAAAACAAGCAATGTTTGATTAACCAGCGTGAAGTTCACCGCCATACCCACAGCTTTAATGC
GGCGCTTAGAAGCGCGCTGCGTGAAGATCCGGACGTGATTTTAGTTGGTGAGATGCGTGACCTTGAAACCATTCGTTTGG
CGATGACCGCCGCAGAAACAGGCCACTTAGTGTTTGGTACCTTGCATACCACCTCCGCCGCGAAAACCATTGACCGTGTG
GTCGACGTATTCCCCGCCGGTGAAAAAGACATGGTGCGCACTATGTTGTCGGAATCGCTGCAAGCGGTTATTTCGCAAAC
CTTGATTAAAAAGGTAGGCGGAGGCCGTGTCGCCGCCCATGAAATCATGATGGGCACTCCCGCTATTCGTAACCTTATTC
GTGAAGACAAAGTGGCGCAGATGTATTCGGCGATTCAAACCGGTATGGCCCACGGAATGCAGACCTTAGAGCAATGTTTA
CAGAACTTAGTCAATCGCGGTCTTATCACCCGTGAAGATGCAATGGCGAAGAGTTCGAATAAACAAGCGACATTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

80.409

99.13

0.797
  pilT Acinetobacter baumannii D1279779

80.409

99.13

0.797
  pilT Acinetobacter baumannii strain A118

80.409

99.13

0.797
  pilT Acinetobacter baylyi ADP1

77.843

99.42

0.774
  pilT Pseudomonas aeruginosa PAK

78.698

97.971

0.771
  pilT Pseudomonas stutzeri DSM 10701

77.485

99.13

0.768
  pilT Legionella pneumophila strain Lp02

75.073

98.841

0.742
  pilT Legionella pneumophila strain ERS1305867

75.073

98.841

0.742
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

73.294

97.681

0.716
  pilT Vibrio cholerae strain A1552

73.294

97.681

0.716
  pilT Neisseria meningitidis 8013

69.767

99.71

0.696
  pilT Neisseria gonorrhoeae MS11

69.477

99.71

0.693
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.558

98.261

0.487
  pilU Pseudomonas stutzeri DSM 10701

41.983

99.42

0.417
  pilU Vibrio cholerae strain A1552

41.399

99.42

0.412
  pilU Acinetobacter baylyi ADP1

39.763

97.681

0.388