Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   OX890_RS16405 Genome accession   NZ_CP113808
Coordinates   3883870..3884982 (-) Length   370 a.a.
NCBI ID   WP_106649931.1    Uniprot ID   -
Organism   Shewanella sp. DAU305     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3878870..3889982
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OX890_RS16370 (OX890_16370) - 3879362..3879922 (-) 561 WP_006082509.1 DUF4136 domain-containing protein -
  OX890_RS16375 (OX890_16375) - 3880101..3880418 (+) 318 WP_268649591.1 hypothetical protein -
  OX890_RS16380 (OX890_16380) yciH 3880597..3880926 (+) 330 WP_219253466.1 stress response translation initiation inhibitor YciH -
  OX890_RS16385 (OX890_16385) - 3880993..3881556 (+) 564 WP_006082512.1 YqgE/AlgH family protein -
  OX890_RS16390 (OX890_16390) ruvX 3881586..3882011 (+) 426 WP_006082513.1 Holliday junction resolvase RuvX -
  OX890_RS16395 (OX890_16395) hemH 3882097..3883110 (-) 1014 WP_268649592.1 ferrochelatase -
  OX890_RS16400 (OX890_16400) - 3883143..3883682 (-) 540 WP_037392614.1 glutathione peroxidase -
  OX890_RS16405 (OX890_16405) pilU 3883870..3884982 (-) 1113 WP_106649931.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OX890_RS16410 (OX890_16410) pilT 3884992..3886029 (-) 1038 WP_006082517.1 type IV pilus twitching motility protein PilT Machinery gene
  OX890_RS16415 (OX890_16415) - 3886065..3886763 (+) 699 WP_268649593.1 YggS family pyridoxal phosphate-dependent enzyme -
  OX890_RS16420 (OX890_16420) proC 3886979..3887797 (+) 819 WP_006082519.1 pyrroline-5-carboxylate reductase -
  OX890_RS16425 (OX890_16425) - 3887846..3888394 (+) 549 WP_006082520.1 YggT family protein -
  OX890_RS16430 (OX890_16430) yggU 3888394..3888693 (+) 300 WP_012587167.1 DUF167 family protein YggU -
  OX890_RS16435 (OX890_16435) - 3888825..3889259 (+) 435 WP_006082522.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40986.13 Da        Isoelectric Point: 6.2352

>NTDB_id=763170 OX890_RS16405 WP_106649931.1 3883870..3884982(-) (pilU) [Shewanella sp. DAU305]
MDVLPFLKVIVERKASDLFITAGFPPSAKIDGELRPLAESAFTPAQSLDFVESLMTEAQKQEFHSSRECNFAFAAKDLGR
FRVSAFWQRESPGCVMRRIETKIPDVDDLKLPPILKDLVMSKRGLIIMVGGTGTGKSTSLAALVGYRNAHARGHILTIED
PVEFVHDHRKSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRTQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHQQLLFDLSLNLRGIVAQQLIPKVDGTGRRAAIEVLINTPRVASLIAKNELHLLKETMAKSNEQGMQTFDQA
LLKLYVDGEISYADALHHADSPNDLRLMIKLQNKESTGSGFMEGVTLDMD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=763170 OX890_RS16405 WP_106649931.1 3883870..3884982(-) (pilU) [Shewanella sp. DAU305]
ATGGATGTTCTTCCGTTTTTAAAAGTGATCGTTGAGCGCAAAGCCTCCGACTTGTTTATCACAGCTGGCTTTCCGCCAAG
TGCCAAGATTGACGGTGAATTGCGTCCCTTGGCCGAAAGTGCCTTTACTCCGGCACAATCACTGGATTTCGTTGAATCCT
TAATGACCGAAGCGCAGAAACAAGAGTTTCACTCGAGCCGTGAGTGTAACTTTGCTTTTGCGGCAAAGGATTTAGGTCGC
TTTCGGGTAAGCGCCTTCTGGCAGCGTGAATCGCCTGGCTGCGTGATGCGCCGTATTGAGACTAAAATCCCTGATGTCGA
TGATTTAAAATTGCCGCCTATTCTGAAAGATCTGGTGATGAGTAAACGTGGTCTCATCATTATGGTGGGCGGAACGGGAA
CGGGTAAGTCAACGTCCTTAGCGGCCTTGGTAGGCTATCGCAATGCCCATGCCCGTGGTCATATTTTAACTATCGAAGAT
CCCGTCGAATTTGTTCACGACCACAGAAAAAGTATTATCACCCAACGTGAAGTCGGCATAGATACTGAGTCTTTTGATGC
GGCGCTAAAAAGCTCCCTTCGCCAAGCCCCCGATGTGATCTTGATTGGTGAAATTCGTACCCAAGAGACGATGGAGTTCG
CCCTGTCGTTTGCAGAAACAGGCCACCTCTGTATGGCAACCTTGCATGCCAACAACGCCAACCAAGCATTAGACCGCATT
ATGCACCTCGTGCCTGAGAGTAAACATCAGCAGTTATTGTTTGATTTGTCATTGAATTTGCGCGGTATTGTGGCGCAGCA
ATTGATCCCAAAAGTCGATGGCACTGGGCGCCGCGCCGCGATTGAAGTCTTGATTAACACCCCAAGGGTGGCGAGTTTAA
TTGCGAAGAATGAGCTGCATTTACTCAAAGAAACCATGGCAAAATCCAACGAGCAGGGCATGCAGACTTTCGATCAAGCC
TTGCTCAAACTTTATGTCGATGGCGAGATTAGTTACGCCGATGCGCTGCACCATGCTGATTCACCGAACGATTTACGTTT
GATGATTAAATTGCAGAATAAAGAATCCACGGGCTCAGGCTTTATGGAAGGTGTCACCTTAGACATGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.946

100

0.603
  pilU Vibrio cholerae strain A1552

58.649

100

0.586
  pilU Acinetobacter baylyi ADP1

60.795

95.135

0.578
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.556

97.297

0.395
  pilT Vibrio cholerae strain A1552

42.727

89.189

0.381
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.727

89.189

0.381
  pilT Acinetobacter baumannii D1279779

39.181

92.432

0.362
  pilT Acinetobacter baumannii strain A118

39.181

92.432

0.362
  pilT Acinetobacter nosocomialis M2

39.181

92.432

0.362
  pilT Legionella pneumophila strain Lp02

40.483

89.459

0.362
  pilT Legionella pneumophila strain ERS1305867

40.483

89.459

0.362