Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OY559_RS04315 Genome accession   NZ_CP113783
Coordinates   956127..957164 (+) Length   345 a.a.
NCBI ID   WP_277728874.1    Uniprot ID   -
Organism   Pseudoxanthomonas sp. SE1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 951127..962164
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OY559_RS04285 (OY559_04285) - 951399..952253 (-) 855 WP_277728869.1 kinase -
  OY559_RS04290 (OY559_04290) pdxH 952286..952888 (+) 603 WP_277728870.1 pyridoxamine 5'-phosphate oxidase -
  OY559_RS04295 (OY559_04295) - 952989..953786 (+) 798 WP_277728871.1 cobalamin-binding protein -
  OY559_RS04300 (OY559_04300) - 953943..954404 (-) 462 WP_277728872.1 DUF4426 domain-containing protein -
  OY559_RS04305 (OY559_04305) proC 954464..955309 (-) 846 WP_277728873.1 pyrroline-5-carboxylate reductase -
  OY559_RS04310 (OY559_04310) - 955350..956033 (-) 684 WP_277729913.1 YggS family pyridoxal phosphate-dependent enzyme -
  OY559_RS04315 (OY559_04315) pilT 956127..957164 (+) 1038 WP_277728874.1 type IV pilus twitching motility protein PilT Machinery gene
  OY559_RS04320 (OY559_04320) pilU 957261..958391 (+) 1131 WP_142123584.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OY559_RS04325 (OY559_04325) - 958574..959083 (+) 510 WP_277728875.1 DUF6622 family protein -
  OY559_RS04330 (OY559_04330) - 959263..960276 (+) 1014 WP_277729914.1 IS110 family transposase -
  OY559_RS04335 (OY559_04335) - 960542..962056 (-) 1515 WP_277729915.1 acetyl-CoA hydrolase/transferase family protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38654.38 Da        Isoelectric Point: 6.7271

>NTDB_id=763034 OY559_RS04315 WP_277728874.1 956127..957164(+) (pilT) [Pseudoxanthomonas sp. SE1]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDFEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGCPPLFRELIQQPQGLILVTGPTGSGKSTTLAAMIDHINKNEYGHILSVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDYILVGELRDLETIRLALTAAETGHLVFATLHTSSAAKTIDRV
IDVFPAGEKPMVRSMLSESLRAVISQALLKKIGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSSIQTGQNYGMMTLDQHL
QDLVKRSLITRQQAKEYAKDKRLFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=763034 OY559_RS04315 WP_277728874.1 956127..957164(+) (pilT) [Pseudoxanthomonas sp. SE1]
ATGGATATCGCCGAACTCTTGGCGTTCTCGGTAAAGAACAAGGCATCGGACCTCCACCTGTCAGCCGGCCTGCCGCCGAT
GATCCGCGTGGACGGCGACGTACGCCGCATCAACATCCCGGCGCTGGACCACAAGCAGGTGCACGCGCTGGTCTACGACA
TCATGTCGGACAAGCAGCGGCGCGACTTCGAGGAATTCCTGGAAGTCGACTTCTCGTTCGAGATCCCGTCGCTGGCGCGC
TTCCGCGTCAACGCGTTCAACCAGAACCGCGGTGCGGGTGCGGTGTTCCGTACCATTCCCTCGGAAGTGCTGACGCTTGA
AGACCTGGGCTGCCCGCCGCTGTTCCGCGAGCTGATCCAGCAGCCGCAGGGCCTGATCCTGGTCACCGGCCCGACCGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGATCGACCACATCAACAAGAACGAATACGGCCACATCCTCAGCGTCGAGGAT
CCGATCGAGTTCGTCCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAAGTGCATCGCGATACGCACGGTTTCAACGA
GGCGCTGCGTTCGGCCCTGCGTGAAGACCCCGACTACATCCTGGTCGGCGAGTTGCGCGACCTGGAAACCATCCGCCTGG
CGTTGACCGCCGCGGAAACCGGCCACCTGGTGTTCGCCACCCTGCATACCAGCTCGGCGGCCAAGACCATCGACCGCGTG
ATCGACGTGTTCCCCGCCGGCGAGAAGCCGATGGTGCGTTCGATGCTGTCCGAATCGCTGCGTGCGGTCATTTCGCAGGC
GCTGCTGAAGAAGATCGGCGGCGGCCGGACTGCCGCGTGGGAAATCATGGTGGGCACGCCCGCCATCCGCAACCTGATCC
GCGAAGACAAGGTGGCGCAGATGTACTCGTCCATCCAGACCGGCCAGAACTACGGAATGATGACCCTGGACCAGCATCTG
CAGGATCTGGTGAAGCGCAGCCTCATCACCCGGCAGCAGGCCAAGGAATACGCGAAGGACAAGCGTCTGTTTGAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

78.779

99.71

0.786
  pilT Acinetobacter baumannii D1279779

78.779

99.71

0.786
  pilT Acinetobacter baumannii strain A118

78.779

99.71

0.786
  pilT Pseudomonas stutzeri DSM 10701

78.488

99.71

0.783
  pilT Pseudomonas aeruginosa PAK

78.171

98.261

0.768
  pilT Acinetobacter baylyi ADP1

76.453

99.71

0.762
  pilT Legionella pneumophila strain Lp02

73.837

99.71

0.736
  pilT Legionella pneumophila strain ERS1305867

73.837

99.71

0.736
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.06

97.101

0.661
  pilT Vibrio cholerae strain A1552

68.06

97.101

0.661
  pilT Neisseria meningitidis 8013

63.93

98.841

0.632
  pilT Neisseria gonorrhoeae MS11

63.636

98.841

0.629
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.565

100

0.496
  pilU Vibrio cholerae strain A1552

40.23

100

0.406
  pilU Pseudomonas stutzeri DSM 10701

41.003

98.261

0.403
  pilU Acinetobacter baylyi ADP1

40.294

98.551

0.397