Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   OY559_RS04320 Genome accession   NZ_CP113783
Coordinates   957261..958391 (+) Length   376 a.a.
NCBI ID   WP_142123584.1    Uniprot ID   -
Organism   Pseudoxanthomonas sp. SE1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 952261..963391
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OY559_RS04290 (OY559_04290) pdxH 952286..952888 (+) 603 WP_277728870.1 pyridoxamine 5'-phosphate oxidase -
  OY559_RS04295 (OY559_04295) - 952989..953786 (+) 798 WP_277728871.1 cobalamin-binding protein -
  OY559_RS04300 (OY559_04300) - 953943..954404 (-) 462 WP_277728872.1 DUF4426 domain-containing protein -
  OY559_RS04305 (OY559_04305) proC 954464..955309 (-) 846 WP_277728873.1 pyrroline-5-carboxylate reductase -
  OY559_RS04310 (OY559_04310) - 955350..956033 (-) 684 WP_277729913.1 YggS family pyridoxal phosphate-dependent enzyme -
  OY559_RS04315 (OY559_04315) pilT 956127..957164 (+) 1038 WP_277728874.1 type IV pilus twitching motility protein PilT Machinery gene
  OY559_RS04320 (OY559_04320) pilU 957261..958391 (+) 1131 WP_142123584.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OY559_RS04325 (OY559_04325) - 958574..959083 (+) 510 WP_277728875.1 DUF6622 family protein -
  OY559_RS04330 (OY559_04330) - 959263..960276 (+) 1014 WP_277729914.1 IS110 family transposase -
  OY559_RS04335 (OY559_04335) - 960542..962056 (-) 1515 WP_277729915.1 acetyl-CoA hydrolase/transferase family protein -
  OY559_RS04340 (OY559_04340) - 962138..962683 (-) 546 WP_277728876.1 DNA-3-methyladenine glycosylase I -
  OY559_RS04345 (OY559_04345) - 962773..963339 (+) 567 WP_277728877.1 YqgE/AlgH family protein -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41850.18 Da        Isoelectric Point: 6.7685

>NTDB_id=763035 OY559_RS04320 WP_142123584.1 957261..958391(+) (pilU) [Pseudoxanthomonas sp. SE1]
MSTIDFTSFLKLMAHQKASDLFITSGMPPSIKVNGKISPITQTPLTSQQSRDLVLNVMTPSQREEFEKTHECNFAIGVAG
VGRFRVSCFYQRNQVGMVLRRIETRIPTIEELNLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWDAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRTQLLMDLSLNLKGVVAQQLIPTPDGKARRVAMEILLGTPLVQDYIREGEVHKLKEVMKESTNLGMKTF
DQALFELYQAGEISYEDALRFADSQNEVRLRIKLAQGGDARTLAQGLDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=763035 OY559_RS04320 WP_142123584.1 957261..958391(+) (pilU) [Pseudoxanthomonas sp. SE1]
ATGAGCACCATCGATTTCACCTCGTTCCTCAAGCTGATGGCGCACCAGAAGGCGTCGGACCTGTTCATCACGTCCGGCAT
GCCGCCATCCATCAAGGTGAACGGCAAGATCTCGCCGATCACGCAGACGCCGCTGACCTCGCAGCAGTCGCGCGACCTGG
TGTTGAACGTGATGACGCCGTCGCAGCGCGAAGAGTTCGAGAAGACCCACGAGTGCAACTTCGCCATCGGCGTGGCCGGC
GTGGGCCGCTTCCGCGTGAGCTGCTTCTACCAGCGCAACCAGGTGGGCATGGTGCTGCGTCGTATCGAGACGCGCATCCC
GACCATCGAAGAGCTGAACCTGCCGCCGGTCATCAAGACGCTGGCCATGACCAAGCGCGGCATCATCATCTTCGTCGGTG
CGACCGGTACGGGTAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAATTCCACCGGCCACATCATCACC
ATCGAAGACCCCATCGAGTTCGTGCACAAGCACGAGGGCTGCATCATCACGCAGCGCGAAGTGGGCATCGACACCGACAG
CTGGGACGCGGCGCTGAAGAACACCCTGCGTCAGGCGCCCGACGTCATCATGATCGGCGAAGTGCGCACGCGCGAAGGCA
TGGACCACGCCATCGCGTTCGCAGAAACCGGCCACCTGGTGCTGTGCACGCTGCACGCCAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCGGAAGACCGCCGCACCCAGCTGCTGATGGACCTGTCACTGAACCTGAAGGGCGTGGT
GGCGCAGCAGCTGATTCCCACCCCCGACGGCAAGGCGCGCCGGGTGGCGATGGAGATCCTGCTGGGCACGCCGCTGGTGC
AGGACTACATCCGCGAAGGCGAGGTGCACAAGCTGAAGGAGGTGATGAAGGAATCCACCAACCTCGGCATGAAGACCTTC
GACCAGGCCCTGTTCGAGCTGTACCAGGCCGGCGAGATCAGCTACGAGGACGCGCTGCGTTTCGCCGACTCGCAGAACGA
AGTCCGCCTGCGCATCAAACTGGCCCAGGGCGGCGATGCACGCACGCTTGCGCAGGGGCTGGATGGGGTGGAGATTGCGG
AAGTCAGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

68.392

97.606

0.668
  pilU Acinetobacter baylyi ADP1

64.146

94.947

0.609
  pilU Vibrio cholerae strain A1552

53.933

94.681

0.511
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.143

93.085

0.402
  pilT Pseudomonas aeruginosa PAK

41.194

89.096

0.367
  pilT Acinetobacter nosocomialis M2

40.116

91.489

0.367
  pilT Acinetobacter baumannii D1279779

40.116

91.489

0.367
  pilT Acinetobacter baumannii strain A118

40.116

91.489

0.367