Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OU800_RS21190 Genome accession   NZ_CP113519
Coordinates   4787977..4789011 (-) Length   344 a.a.
NCBI ID   WP_268179316.1    Uniprot ID   -
Organism   Pseudomonas sp. GOM7     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4782977..4794011
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OU800_RS21165 (OU800_21165) - 4783336..4783986 (-) 651 WP_268179311.1 DUF2076 domain-containing protein -
  OU800_RS21170 (OU800_21170) - 4784095..4784493 (-) 399 WP_268179312.1 TM2 domain-containing protein -
  OU800_RS21175 (OU800_21175) - 4784641..4785852 (+) 1212 WP_268179313.1 putative DNA modification/repair radical SAM protein -
  OU800_RS21180 (OU800_21180) - 4785852..4786739 (+) 888 WP_268179314.1 TIGR03915 family putative DNA repair protein -
  OU800_RS21185 (OU800_21185) pilU 4786705..4787850 (-) 1146 WP_268179315.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OU800_RS21190 (OU800_21190) pilT 4787977..4789011 (-) 1035 WP_268179316.1 type IV pilus twitching motility protein PilT Machinery gene
  OU800_RS21195 (OU800_21195) - 4789077..4789769 (+) 693 WP_268179317.1 YggS family pyridoxal phosphate-dependent enzyme -
  OU800_RS21200 (OU800_21200) proC 4789787..4790602 (+) 816 WP_268179318.1 pyrroline-5-carboxylate reductase -
  OU800_RS21205 (OU800_21205) - 4790657..4791250 (+) 594 WP_268179319.1 YggT family protein -
  OU800_RS21210 (OU800_21210) - 4791253..4791543 (+) 291 WP_268179320.1 DUF167 domain-containing protein -
  OU800_RS21215 (OU800_21215) - 4791756..4793723 (+) 1968 WP_268179321.1 dynamin-like GTPase family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37885.58 Da        Isoelectric Point: 6.8887

>NTDB_id=762692 OU800_RS21190 WP_268179316.1 4787977..4789011(-) (pilT) [Pseudomonas sp. GOM7]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPLDHKQVHALIYDIMNDKQRKDYEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMDDLGMGEVFRKITDVPRGLVLVTGPTGSGKSTTLAAMLDYLNSNKYHHILTIED
PIEFVHESKKCLVNQREVHRDTHGFSEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMVRSMLSESLQAVISQTLLKKIGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGGALGMQTLDACL
KTLVAKGLVSRDSAREKAKSPENL

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=762692 OU800_RS21190 WP_268179316.1 4787977..4789011(-) (pilT) [Pseudomonas sp. GOM7]
ATGGATATTACCGAGCTGCTTGCCTTCAGCGCCAAGCAAGGCGCGTCGGATTTGCACCTCTCGGCCGGTTTGCCGCCGAT
GATCCGCGTCGACGGCGACGTGCGCCGCATCAACCTGCCACCGCTGGATCACAAGCAGGTGCACGCGCTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGATTACGAGGAATTCCTCGAGACCGACTTCTCCTTCGAGGTGCCGGGTGTCGCACGC
TTCCGGGTCAACGCCTTCAATCAGAACCGGGGCGCCGGTGCGGTGTTCCGTACCATTCCGTCCAAGGTGCTGACCATGGA
CGACCTGGGCATGGGTGAGGTATTCCGCAAGATCACCGACGTGCCGCGCGGCCTGGTGCTGGTCACCGGCCCCACCGGCT
CGGGCAAGTCGACCACCCTGGCGGCGATGCTCGACTACCTCAATAGCAACAAGTACCACCATATCCTCACCATCGAAGAC
CCCATCGAATTCGTCCACGAGTCGAAGAAGTGCCTGGTCAACCAGCGCGAGGTGCACCGCGACACCCATGGTTTCTCCGA
AGCCCTGCGCTCGGCGCTGCGGGAAGACCCGGACATCATCCTGGTGGGCGAGATGCGTGACCTGGAGACCATCCGCCTGG
CGCTGACCGCGGCGGAAACCGGCCACCTGGTATTCGGCACCCTGCACACCACCTCCGCGGCCAAGACCATCGACCGCGTG
GTCGACGTGTTCCCCGCCGAGGAAAAATCCATGGTGCGCTCGATGCTGTCCGAATCGCTGCAGGCGGTGATCTCGCAGAC
CCTGCTGAAGAAGATCGGTGGTGGTCGCGTGGCGGCCCACGAAATCATGATCGGTACGCCGGCCATCCGTAACCTGATCC
GTGAGGACAAGGTGGCGCAGATGTATTCGGCGATCCAGACTGGAGGCGCGCTGGGTATGCAGACCCTCGATGCCTGCCTC
AAGACCCTGGTGGCCAAGGGCCTGGTCAGCCGCGACAGCGCGCGGGAAAAGGCCAAGAGTCCGGAAAACTTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

94.767

100

0.948
  pilT Pseudomonas aeruginosa PAK

93.605

100

0.936
  pilT Acinetobacter baumannii D1279779

84.593

100

0.846
  pilT Acinetobacter baumannii strain A118

84.593

100

0.846
  pilT Acinetobacter nosocomialis M2

84.302

100

0.843
  pilT Acinetobacter baylyi ADP1

81.977

100

0.82
  pilT Legionella pneumophila strain Lp02

75.148

98.256

0.738
  pilT Legionella pneumophila strain ERS1305867

75.148

98.256

0.738
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.436

97.965

0.68
  pilT Vibrio cholerae strain A1552

69.436

97.965

0.68
  pilT Neisseria meningitidis 8013

66.667

100

0.669
  pilT Neisseria gonorrhoeae MS11

66.377

100

0.666
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

100

0.52
  pilU Pseudomonas stutzeri DSM 10701

40.29

100

0.404
  pilU Vibrio cholerae strain A1552

40.29

100

0.404
  pilU Acinetobacter baylyi ADP1

39.884

100

0.401