Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NM686_RS00855 Genome accession   NZ_CP113517
Coordinates   185348..186385 (-) Length   345 a.a.
NCBI ID   WP_255190055.1    Uniprot ID   -
Organism   Methylomonas rapida strain MP1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 180348..191385
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NM686_RS00835 (NM686_000835) bluB 180915..181565 (+) 651 WP_255190051.1 5,6-dimethylbenzimidazole synthase -
  NM686_RS00840 (NM686_000840) - 181555..183024 (+) 1470 WP_255190052.1 cobyric acid synthase -
  NM686_RS00845 (NM686_000845) mltB 183029..184192 (-) 1164 WP_255190053.1 lytic murein transglycosylase B -
  NM686_RS00850 (NM686_000850) pilU 184199..185332 (-) 1134 WP_255190054.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NM686_RS00855 (NM686_000855) pilT 185348..186385 (-) 1038 WP_255190055.1 type IV pilus twitching motility protein PilT Machinery gene
  NM686_RS00860 (NM686_000860) - 186508..186849 (-) 342 WP_255190056.1 TraR/DksA family transcriptional regulator -
  NM686_RS00865 (NM686_000865) - 187041..188267 (+) 1227 WP_255190057.1 NADP-dependent isocitrate dehydrogenase -
  NM686_RS00870 (NM686_000870) - 188302..188895 (-) 594 WP_255190058.1 DUF502 domain-containing protein -
  NM686_RS00875 (NM686_000875) - 189015..190151 (+) 1137 WP_269022883.1 efflux RND transporter periplasmic adaptor subunit -
  NM686_RS00880 (NM686_000880) - 190148..190810 (+) 663 WP_255190060.1 ABC transporter ATP-binding protein -
  NM686_RS00885 (NM686_000885) - 191097..191258 (+) 162 WP_255190061.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38233.12 Da        Isoelectric Point: 6.8362

>NTDB_id=762642 NM686_RS00855 WP_255190055.1 185348..186385(-) (pilT) [Methylomonas rapida strain MP1]
MDIAELLTFSVKNKASDLHLSAGLPPMIRVDGDIRRINIPALDHKEVHALIYDIMNDKQRRDYEEFLETDFSFALPGVAR
FRVNAFNQDRGAGAVFRTIPSKVLTLEELGAPKFFEELCTKPRGLILVTGPTGSGKSTTLAAMINHINTNDYAHILTVED
PIEFVHESQKCLVNQREVHRDTLGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
IDVFPAAEKDMIRSMLSESLQAVISQTLLKKVGGGRIAAHEIMVGTAAIRNLIREAKVAQMYSAIQTGRKDGMQTLDQNL
KEMVDKGLITAKSAMVKAVNKDLFR

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=762642 NM686_RS00855 WP_255190055.1 185348..186385(-) (pilT) [Methylomonas rapida strain MP1]
ATGGACATCGCCGAATTATTGACTTTTTCCGTTAAAAACAAAGCCTCGGACTTGCATTTGTCCGCCGGTTTACCTCCCAT
GATTCGGGTCGACGGCGATATCCGTCGCATCAATATCCCGGCCTTGGATCACAAGGAAGTCCATGCGCTGATCTATGACA
TCATGAACGACAAGCAGCGCCGCGATTACGAGGAATTCCTGGAAACCGACTTTTCCTTCGCCCTGCCCGGCGTCGCCCGC
TTTCGTGTCAACGCGTTCAATCAGGACAGAGGCGCGGGCGCTGTGTTCAGGACCATTCCTTCCAAGGTCTTGACGCTGGA
AGAACTGGGGGCGCCGAAATTTTTCGAAGAGCTCTGCACCAAGCCGCGCGGCCTGATTCTGGTGACCGGTCCGACCGGTT
CCGGTAAATCCACCACGCTGGCGGCGATGATCAACCATATCAATACCAACGATTATGCCCACATCCTGACGGTCGAAGAT
CCGATCGAGTTCGTGCATGAAAGCCAGAAATGCCTGGTCAACCAACGCGAGGTGCACCGGGACACCCTCGGCTTCAACGA
AGCCTTGCGCTCGGCGCTGAGGGAAGATCCGGACATCATCCTGGTCGGCGAGATGCGGGACCTGGAAACCATCCGTCTGG
CCCTGACCGCGGCGGAAACCGGCCACTTGGTGTTCGGCACCCTGCACACCACCTCGGCGGCGAAAACCATAGACCGTATC
ATCGACGTGTTCCCAGCCGCGGAAAAAGACATGATCCGCTCGATGCTGTCTGAATCCTTGCAGGCCGTCATCTCGCAAAC
ACTGCTGAAAAAAGTCGGCGGTGGCCGGATTGCCGCCCACGAAATCATGGTCGGCACTGCGGCGATCCGCAATCTGATCC
GGGAAGCCAAGGTCGCGCAAATGTATTCGGCAATCCAGACCGGCCGCAAGGACGGCATGCAGACCCTGGATCAAAATTTA
AAAGAAATGGTCGACAAGGGCTTGATCACCGCCAAATCGGCCATGGTCAAAGCCGTCAACAAAGATTTGTTCCGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

80.233

99.71

0.8
  pilT Pseudomonas aeruginosa PAK

81.065

97.971

0.794
  pilT Acinetobacter baylyi ADP1

79.651

99.71

0.794
  pilT Acinetobacter baumannii strain A118

79.36

99.71

0.791
  pilT Acinetobacter baumannii D1279779

79.36

99.71

0.791
  pilT Acinetobacter nosocomialis M2

79.36

99.71

0.791
  pilT Legionella pneumophila strain Lp02

73.837

99.71

0.736
  pilT Legionella pneumophila strain ERS1305867

73.837

99.71

0.736
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.139

97.681

0.675
  pilT Vibrio cholerae strain A1552

69.139

97.681

0.675
  pilT Neisseria meningitidis 8013

66.181

99.42

0.658
  pilT Neisseria gonorrhoeae MS11

65.889

99.42

0.655
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.725

100

0.507
  pilU Acinetobacter baylyi ADP1

40.588

98.551

0.4
  pilU Pseudomonas stutzeri DSM 10701

39.884

100

0.4
  pilU Vibrio cholerae strain A1552

40.413

98.261

0.397