Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NM686_RS00850 Genome accession   NZ_CP113517
Coordinates   184199..185332 (-) Length   377 a.a.
NCBI ID   WP_255190054.1    Uniprot ID   -
Organism   Methylomonas rapida strain MP1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 179199..190332
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NM686_RS00825 (NM686_000825) - 179729..180142 (+) 414 WP_255190049.1 TM2 domain-containing protein -
  NM686_RS00830 (NM686_000830) - 180196..180918 (+) 723 WP_255190050.1 adenosylcobinamide-GDP ribazoletransferase -
  NM686_RS00835 (NM686_000835) bluB 180915..181565 (+) 651 WP_255190051.1 5,6-dimethylbenzimidazole synthase -
  NM686_RS00840 (NM686_000840) - 181555..183024 (+) 1470 WP_255190052.1 cobyric acid synthase -
  NM686_RS00845 (NM686_000845) mltB 183029..184192 (-) 1164 WP_255190053.1 lytic murein transglycosylase B -
  NM686_RS00850 (NM686_000850) pilU 184199..185332 (-) 1134 WP_255190054.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NM686_RS00855 (NM686_000855) pilT 185348..186385 (-) 1038 WP_255190055.1 type IV pilus twitching motility protein PilT Machinery gene
  NM686_RS00860 (NM686_000860) - 186508..186849 (-) 342 WP_255190056.1 TraR/DksA family transcriptional regulator -
  NM686_RS00865 (NM686_000865) - 187041..188267 (+) 1227 WP_255190057.1 NADP-dependent isocitrate dehydrogenase -
  NM686_RS00870 (NM686_000870) - 188302..188895 (-) 594 WP_255190058.1 DUF502 domain-containing protein -
  NM686_RS00875 (NM686_000875) - 189015..190151 (+) 1137 WP_269022883.1 efflux RND transporter periplasmic adaptor subunit -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 42314.63 Da        Isoelectric Point: 6.3897

>NTDB_id=762641 NM686_RS00850 WP_255190054.1 184199..185332(-) (pilU) [Methylomonas rapida strain MP1]
MDFKALLALMVEKKASDLFITAGKPPCMKIDGKVVEVSKNVLNSDQAMRVVLSIMDQRHKDEFEHTKECQFALGVEGLGR
FRVSAFTQRDAAGMVLRRIETTVPTSEELHLPPVLKELIMYKRGLVLFVGATGTGKSTSLAALIRHRNENSNGHIITIED
PIEFVHPHLGCIITQREVGLDTESYEVALKNTLRQAPDVILIGEVRTKETMQHAITFSETGHLCLCTLHANNANQALDRI
LHFFPEDMHSQIFMDLSLNLRGIVAQQLVKRTDGKGRYPAIEILLNTPLVSDLIRKGEVHKLKELMKNSREHGMQTFDQA
LYDLYTAGKISYEDALNAADSRNEVRLMIKLGAENANFDNDEMRLTETDEDGGSLYK

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=762641 NM686_RS00850 WP_255190054.1 184199..185332(-) (pilU) [Methylomonas rapida strain MP1]
ATGGATTTCAAAGCATTATTGGCCTTGATGGTGGAAAAAAAGGCCTCGGATTTATTCATCACCGCCGGCAAACCGCCGTG
CATGAAAATCGACGGCAAAGTCGTTGAAGTTTCCAAAAACGTGCTGAACTCCGATCAAGCGATGCGCGTGGTGCTCAGCA
TCATGGATCAACGGCATAAGGACGAATTCGAACATACCAAGGAATGTCAGTTTGCCTTGGGCGTGGAAGGCCTGGGCCGC
TTCCGTGTCAGCGCCTTTACCCAACGCGATGCCGCCGGCATGGTGCTGCGCCGCATCGAAACCACCGTTCCGACCTCGGA
AGAACTGCACTTGCCGCCCGTTCTGAAAGAACTGATCATGTACAAACGCGGCCTGGTATTGTTCGTCGGCGCCACCGGCA
CCGGTAAATCGACCTCGCTGGCGGCCTTGATACGCCACCGTAACGAGAACAGCAACGGCCATATCATCACGATAGAAGAC
CCCATCGAGTTCGTGCACCCGCATCTGGGTTGCATCATCACCCAGCGCGAGGTGGGCTTGGATACCGAGTCTTACGAAGT
GGCGCTGAAAAACACCCTGCGCCAAGCGCCGGACGTGATTCTGATCGGCGAGGTCCGCACCAAGGAAACCATGCAGCATG
CGATCACCTTCTCCGAGACTGGCCACTTATGCTTATGCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGGATT
CTGCATTTCTTCCCGGAAGACATGCACAGTCAAATCTTCATGGATTTATCGCTTAACCTGCGCGGCATCGTTGCCCAACA
ACTGGTCAAGCGTACCGATGGCAAGGGCCGTTATCCGGCGATCGAAATCCTGCTGAACACGCCGTTGGTTTCCGATTTGA
TCCGCAAGGGCGAAGTGCATAAACTGAAGGAATTGATGAAAAACTCGCGCGAGCATGGCATGCAAACCTTCGACCAGGCC
TTGTACGACTTGTACACCGCCGGCAAGATCAGCTATGAAGACGCCTTGAATGCCGCCGACTCCCGCAACGAAGTCAGGTT
GATGATCAAACTGGGCGCTGAAAACGCGAATTTCGATAACGACGAAATGAGGCTGACCGAAACCGACGAAGACGGTGGCA
GCCTGTATAAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.797

93.899

0.637
  pilU Acinetobacter baylyi ADP1

61.995

98.408

0.61
  pilU Vibrio cholerae strain A1552

57.429

92.838

0.533
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.391

92.308

0.401
  pilT Legionella pneumophila strain Lp02

41.593

89.92

0.374
  pilT Legionella pneumophila strain ERS1305867

41.593

89.92

0.374
  pilT Acinetobacter nosocomialis M2

41.298

89.92

0.371
  pilT Acinetobacter baumannii strain A118

41.298

89.92

0.371
  pilT Acinetobacter baumannii D1279779

41.298

89.92

0.371
  pilT Pseudomonas aeruginosa PAK

41.003

89.92

0.369
  pilT Vibrio cholerae strain A1552

41.317

88.594

0.366
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.317

88.594

0.366
  pilT Acinetobacter baylyi ADP1

40.176

90.451

0.363