Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OU419_RS02210 Genome accession   NZ_CP113432
Coordinates   468304..469338 (+) Length   344 a.a.
NCBI ID   WP_254470063.1    Uniprot ID   -
Organism   Pseudomonas triclosanedens strain ZM23     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 463304..474338
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OU419_RS02185 (OU419_02185) - 463702..465669 (-) 1968 WP_254470058.1 dynamin-like GTPase family protein -
  OU419_RS02190 (OU419_02190) - 465787..466077 (-) 291 WP_254470059.1 DUF167 domain-containing protein -
  OU419_RS02195 (OU419_02195) - 466083..466676 (-) 594 WP_254470060.1 YggT family protein -
  OU419_RS02200 (OU419_02200) proC 466690..467523 (-) 834 WP_254470061.1 pyrroline-5-carboxylate reductase -
  OU419_RS02205 (OU419_02205) - 467541..468230 (-) 690 WP_254470062.1 YggS family pyridoxal phosphate-dependent enzyme -
  OU419_RS02210 (OU419_02210) pilT 468304..469338 (+) 1035 WP_254470063.1 type IV pilus twitching motility protein PilT Machinery gene
  OU419_RS02215 (OU419_02215) pilU 469441..470586 (+) 1146 WP_254470064.1 type IV pilus ATPase PilU Machinery gene
  OU419_RS02220 (OU419_02220) - 470656..471060 (+) 405 WP_254470065.1 TM2 domain-containing protein -
  OU419_RS02225 (OU419_02225) - 471276..472649 (+) 1374 WP_254470066.1 cystathionine beta-synthase -
  OU419_RS02230 (OU419_02230) - 472646..473815 (+) 1170 WP_254470067.1 PLP-dependent aspartate aminotransferase family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37884.73 Da        Isoelectric Point: 6.7752

>NTDB_id=761939 OU419_RS02210 WP_254470063.1 468304..469338(+) (pilT) [Pseudomonas triclosanedens strain ZM23]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPLEHKQVHALIYDIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGEVFKRVSDVPRGLVLVTGPTGSGKSTSLAAMLDYLNNTKYSHILTVED
PIEFVHESKKCLVNQREVHRDTLGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
VDVFPAEEKSMVRSMLSESLQAVISQTLLKKIGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSSIQTGGAIGMQTLDMCL
KGLVAKGLVTRDAAKEKAKIPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=761939 OU419_RS02210 WP_254470063.1 468304..469338(+) (pilT) [Pseudomonas triclosanedens strain ZM23]
ATGGATATTACCGAGTTGCTCGCCTTCAGCGCCAAGCAAGGCGCTTCGGACCTGCACCTCTCGGCCGGTCTGCCGCCGAT
GATCCGCGTCGACGGCGATGTCCGCCGGATCAACCTGCCACCCCTGGAGCACAAGCAGGTTCACGCGCTGATCTACGACA
TCATGAATGACAAGCAGCGCAAGGATTTCGAGGAATTCCTGGAGACCGACTTCTCGTTCGAAGTCCCCGGGGTCGCCCGT
TTCCGGGTCAACGCATTCAACCAGAACCGCGGCGCCGGCGCCGTGTTCCGGACCATTCCCTCCAAGGTACTCACGATGGA
GGACCTGGGCATGGGAGAAGTGTTCAAACGTGTTTCAGATGTCCCGCGCGGCCTGGTGCTGGTCACCGGCCCGACCGGTT
CCGGCAAGTCCACCTCGCTCGCCGCGATGCTCGACTACCTGAACAACACCAAGTACAGCCACATCCTCACTGTCGAAGAC
CCCATCGAATTCGTCCACGAATCGAAGAAGTGCCTGGTCAACCAGCGCGAGGTGCACCGTGACACGCTGGGCTTCAACGA
GGCCCTGCGCTCGGCGCTGCGTGAAGACCCGGACATCATCCTGGTGGGCGAGATGCGTGACCTGGAGACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGCCACCTGGTATTTGGCACCCTGCACACCACCTCCGCGGCCAAGACCATCGACCGTATC
GTCGACGTGTTCCCCGCCGAAGAAAAATCGATGGTCCGCTCCATGCTCTCCGAATCTCTGCAGGCGGTGATTTCCCAGAC
CCTGCTGAAGAAGATCGGTGGCGGCCGGGTGGCGGCCCACGAGATCATGATCGGCACCCCGGCCATTCGTAACCTGATCC
GTGAGGACAAGGTCGCACAGATGTACTCGTCGATCCAGACCGGCGGTGCGATCGGCATGCAGACTCTCGACATGTGCCTC
AAGGGCCTGGTCGCCAAGGGGCTGGTCACCCGTGACGCGGCGAAAGAAAAAGCCAAGATCCCGGAAAACTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

95.058

100

0.951
  pilT Pseudomonas stutzeri DSM 10701

93.605

100

0.936
  pilT Acinetobacter baumannii D1279779

84.012

100

0.84
  pilT Acinetobacter baumannii strain A118

84.012

100

0.84
  pilT Acinetobacter nosocomialis M2

83.721

100

0.837
  pilT Acinetobacter baylyi ADP1

82.267

100

0.823
  pilT Legionella pneumophila strain Lp02

76.331

98.256

0.75
  pilT Legionella pneumophila strain ERS1305867

76.331

98.256

0.75
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.529

98.837

0.677
  pilT Vibrio cholerae strain A1552

68.529

98.837

0.677
  pilT Neisseria gonorrhoeae MS11

66.087

100

0.663
  pilT Neisseria meningitidis 8013

66.087

100

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.754

100

0.529
  pilU Vibrio cholerae strain A1552

41.003

98.547

0.404
  pilU Pseudomonas stutzeri DSM 10701

41.194

97.384

0.401
  pilU Acinetobacter baylyi ADP1

39.017

100

0.392