Detailed information NTG0761940

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: OU419_RS02215
• Genome accession: NZ_CP113432
• Coordinates: 469441..470586 (+)
• Length: 381 a.a.
• NCBI ID: WP_254470064.1
• Uniprot ID: -
• Organism: Pseudomonas triclosanedens strain ZM23
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 464441..475586

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OU419_RS02190 (OU419_02190)	-	465787..466077 (-)	291	WP_254470059.1	DUF167 domain-containing protein	-
OU419_RS02195 (OU419_02195)	-	466083..466676 (-)	594	WP_254470060.1	YggT family protein	-
OU419_RS02200 (OU419_02200)	proC	466690..467523 (-)	834	WP_254470061.1	pyrroline-5-carboxylate reductase	-
OU419_RS02205 (OU419_02205)	-	467541..468230 (-)	690	WP_254470062.1	YggS family pyridoxal phosphate-dependent enzyme	-
OU419_RS02210 (OU419_02210)	pilT	468304..469338 (+)	1035	WP_254470063.1	type IV pilus twitching motility protein PilT	Machinery gene
OU419_RS02215 (OU419_02215)	pilU	469441..470586 (+)	1146	WP_254470064.1	type IV pilus ATPase PilU	Machinery gene
OU419_RS02220 (OU419_02220)	-	470656..471060 (+)	405	WP_254470065.1	TM2 domain-containing protein	-
OU419_RS02225 (OU419_02225)	-	471276..472649 (+)	1374	WP_254470066.1	cystathionine beta-synthase	-
OU419_RS02230 (OU419_02230)	-	472646..473815 (+)	1170	WP_254470067.1	PLP-dependent aspartate aminotransferase family protein	-
OU419_RS02235 (OU419_02235)	-	473991..475262 (-)	1272	WP_254470068.1	dihydroorotase	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42491.81 Da
• Isoelectric Point: 6.7257

>NTDB_id=761940 OU419_RS02215 WP_254470064.1 469441..470586(+) (pilU) [Pseudomonas triclosanedens strain ZM23]
MEFEKLLRLMVEKGASDLFITAGVPPSMKVNGKVLPVTKTALSPEQTRETVLSVMNEQQRRDFAENHECNFAISARGVGR
FRVSAFYQRNLVGMVLRRIETSIPTIEDLKLPEILKKLAMTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAYADSANDLRLMIKLGSETDADHLSTMTQGLTLELSDDDPNRRLR

Nucleotide

• Length: 1146 bp

>NTDB_id=761940 OU419_RS02215 WP_254470064.1 469441..470586(+) (pilU) [Pseudomonas triclosanedens strain ZM23]
ATGGAATTCGAGAAACTGCTGCGCCTGATGGTGGAAAAGGGAGCTTCCGACCTTTTCATCACCGCCGGCGTTCCCCCGTC
GATGAAGGTCAACGGCAAGGTGTTGCCAGTGACCAAGACTGCGCTGTCGCCGGAGCAGACCCGTGAGACCGTGCTCTCGG
TGATGAACGAGCAGCAGCGCCGCGACTTCGCCGAGAACCACGAGTGCAACTTCGCCATCAGCGCCCGCGGAGTCGGCCGT
TTCCGGGTCAGCGCCTTTTACCAGCGCAACCTGGTGGGCATGGTGCTGCGTCGGATCGAGACCAGTATCCCGACCATCGA
GGATCTGAAGCTTCCGGAAATCCTCAAGAAGCTGGCGATGACCAAGCGCGGCCTGGTGATCTTTGTCGGTGCCACCGGCA
CCGGTAAGTCCACGTCGCTGGCGGCGATGATCGGCTATCGCAACAAGAACTCCACCGGCCACATCATCTCCATCGAAGAC
CCCATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGTGAGGTGGGCCTGGATACCGATTCCTTCGAAGT
TGCCTTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGTGAGGTGCGCTCCCGTGAGACGATGGACCACG
CCGTGGCCTTCGCCGAAACCGGCCACCTCTGCCTGGCCACGCTGCACGCCAACAACGCCAACCAGGCGCTGGAGCGGATC
ATTCACTTCTTTCCGGCGGATCGTCACGGGCAGGTGTGGATGGACCTGTCGTTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCGGACGGCAAGGGCCGTCGCGCGGTGATCGAGGTGCTGCTCAATACCCCGCTGGCAGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTCAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGTATGCAGACCTTCGACCAGGCG
CTGTATCAGCTCTACACCCAGGGCGAGATCACCTATGAGGATGCGCTGGCCTACGCCGACTCGGCGAACGACCTGCGCCT
GATGATCAAGCTGGGCTCGGAAACCGACGCCGACCATCTCTCCACCATGACCCAGGGGCTGACCCTGGAACTCAGCGACG
ACGACCCCAACCGGCGCCTGCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (112-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	87.927	100	0.879
pilU	Acinetobacter baylyi ADP1	63.564	98.688	0.627
pilU	Vibrio cholerae strain A1552	60.857	91.864	0.559
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.363	88.976	0.386