Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OOK74_RS01780 Genome accession   NZ_CP113241
Coordinates   364829..365866 (+) Length   345 a.a.
NCBI ID   WP_278183000.1    Uniprot ID   -
Organism   Vibrio sp. gvc     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 359829..370866
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OOK74_RS01750 hemW 360461..361636 (-) 1176 WP_278182996.1 radical SAM family heme chaperone HemW -
  OOK74_RS01755 - 361630..362232 (-) 603 WP_278182997.1 XTP/dITP diphosphatase -
  OOK74_RS01760 yggU 362317..362607 (-) 291 WP_278182998.1 DUF167 family protein YggU -
  OOK74_RS01765 - 362607..363164 (-) 558 WP_274723627.1 YggT family protein -
  OOK74_RS01770 proC 363221..364039 (-) 819 WP_278182999.1 pyrroline-5-carboxylate reductase -
  OOK74_RS01775 - 364096..364803 (-) 708 WP_274723625.1 YggS family pyridoxal phosphate-dependent enzyme -
  OOK74_RS01780 pilT 364829..365866 (+) 1038 WP_278183000.1 type IV pilus twitching motility protein PilT Machinery gene
  OOK74_RS01785 pilU 365880..366986 (+) 1107 WP_274723623.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OOK74_RS01790 - 367065..367862 (+) 798 WP_278183001.1 helix-turn-helix transcriptional regulator -
  OOK74_RS01795 tyrS 367984..369270 (+) 1287 WP_278183002.1 tyrosine--tRNA ligase -
  OOK74_RS01800 ruvX 369328..369750 (-) 423 WP_274723620.1 Holliday junction resolvase RuvX -
  OOK74_RS01805 - 369820..370383 (-) 564 WP_278183003.1 YqgE/AlgH family protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38226.68 Da        Isoelectric Point: 6.5041

>NTDB_id=761086 OOK74_RS01780 WP_278183000.1 364829..365866(+) (pilT) [Vibrio sp. gvc]
MDIAELLEFSVKHNASDLHLSAGVPPMVRIDGEVRKLGVPAFSHSEVHRLVFEIMNDAQRSEYEEKLEVDFSFELPNVGR
FRVNAFHQARGASAVFRTIPSVIPSLEQLEAPEIFSKIANYEKGLVLVTGPTGSGKSTTLAAMVDYVNAHHNKHILTIED
PIEFVHSNNKCLINQREVHRDTHSFKNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRAVIAQNLLKRVGGGRIACHEIMLATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
RQLIARGVVDAEQAQHKIEMDLKGF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=761086 OOK74_RS01780 WP_278183000.1 364829..365866(+) (pilT) [Vibrio sp. gvc]
ATGGATATCGCCGAGTTACTGGAGTTCAGTGTAAAGCATAACGCATCCGATCTACACCTTTCCGCAGGTGTTCCACCTAT
GGTACGGATTGATGGCGAAGTTAGGAAGCTTGGCGTGCCGGCTTTTAGTCATTCTGAGGTGCATCGCTTGGTGTTTGAAA
TCATGAATGATGCCCAGCGCAGTGAGTATGAAGAGAAACTGGAAGTCGATTTCTCCTTTGAGTTGCCGAATGTGGGGCGC
TTTCGGGTCAACGCATTCCATCAAGCGCGTGGCGCTTCGGCGGTATTTCGTACCATTCCAAGCGTGATCCCCAGCTTAGA
GCAGCTCGAGGCTCCAGAAATTTTCAGCAAAATCGCCAATTATGAAAAAGGCTTAGTCTTGGTGACTGGGCCAACCGGAT
CGGGCAAATCAACCACGTTGGCGGCAATGGTCGATTACGTCAATGCTCACCATAACAAGCATATTTTAACCATTGAAGAT
CCGATTGAGTTTGTGCACAGCAACAACAAATGTTTGATTAATCAACGTGAAGTTCATCGTGATACCCACAGTTTTAAAAA
TGCGCTGCGCTCGGCGCTGCGTGAAGATCCGGATGTGATTTTAGTCGGAGAGCTGCGCGATCAAGAAACCATCAGTTTGG
CGCTCACCGCAGCAGAAACTGGGCACTTGGTGTTTGGTACTTTGCACACCAGCTCGGCGGCCAAAACCATTGACCGGATC
ATCGATGTTTTCCCAGGCAGTGATAAAGATATGGTGCGCTCCATGCTCTCGGAATCGCTGCGTGCGGTCATTGCACAGAA
TCTGCTCAAACGGGTCGGCGGTGGGCGGATCGCTTGTCATGAGATCATGTTGGCTACGCCTGCGATCCGCAATTTGATCC
GCGAAGATAAAGTGGCGCAGATGTATTCGATTATTCAAACTGGCGCGGCACATGGTATGCAAACCATGGAGCAAAATGCC
AGACAGCTGATCGCCCGTGGAGTGGTCGATGCTGAGCAAGCACAGCACAAAATCGAAATGGATTTAAAAGGATTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

95.072

100

0.951
  pilT Vibrio cholerae strain A1552

95.072

100

0.951
  pilT Acinetobacter baumannii D1279779

71.94

97.101

0.699
  pilT Acinetobacter baumannii strain A118

71.94

97.101

0.699
  pilT Acinetobacter nosocomialis M2

71.642

97.101

0.696
  pilT Acinetobacter baylyi ADP1

71.642

97.101

0.696
  pilT Pseudomonas stutzeri DSM 10701

68.843

97.681

0.672
  pilT Pseudomonas aeruginosa PAK

67.059

98.551

0.661
  pilT Legionella pneumophila strain Lp02

67.066

96.812

0.649
  pilT Legionella pneumophila strain ERS1305867

67.066

96.812

0.649
  pilT Neisseria meningitidis 8013

64.412

98.551

0.635
  pilT Neisseria gonorrhoeae MS11

64.118

98.551

0.632
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.201

92.174

0.481
  pilU Vibrio cholerae strain A1552

41.543

97.681

0.406
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.101

0.397
  pilU Acinetobacter baylyi ADP1

40.312

92.754

0.374