Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   OOK74_RS01785 Genome accession   NZ_CP113241
Coordinates   365880..366986 (+) Length   368 a.a.
NCBI ID   WP_274723623.1    Uniprot ID   -
Organism   Vibrio sp. gvc     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 360880..371986
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OOK74_RS01755 - 361630..362232 (-) 603 WP_278182997.1 XTP/dITP diphosphatase -
  OOK74_RS01760 yggU 362317..362607 (-) 291 WP_278182998.1 DUF167 family protein YggU -
  OOK74_RS01765 - 362607..363164 (-) 558 WP_274723627.1 YggT family protein -
  OOK74_RS01770 proC 363221..364039 (-) 819 WP_278182999.1 pyrroline-5-carboxylate reductase -
  OOK74_RS01775 - 364096..364803 (-) 708 WP_274723625.1 YggS family pyridoxal phosphate-dependent enzyme -
  OOK74_RS01780 pilT 364829..365866 (+) 1038 WP_278183000.1 type IV pilus twitching motility protein PilT Machinery gene
  OOK74_RS01785 pilU 365880..366986 (+) 1107 WP_274723623.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OOK74_RS01790 - 367065..367862 (+) 798 WP_278183001.1 helix-turn-helix transcriptional regulator -
  OOK74_RS01795 tyrS 367984..369270 (+) 1287 WP_278183002.1 tyrosine--tRNA ligase -
  OOK74_RS01800 ruvX 369328..369750 (-) 423 WP_274723620.1 Holliday junction resolvase RuvX -
  OOK74_RS01805 - 369820..370383 (-) 564 WP_278183003.1 YqgE/AlgH family protein -
  OOK74_RS01810 gshB 370418..371374 (-) 957 WP_166013706.1 glutathione synthase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41443.38 Da        Isoelectric Point: 6.5052

>NTDB_id=761087 OOK74_RS01785 WP_274723623.1 365880..366986(+) (pilU) [Vibrio sp. gvc]
MDLNQYLDGMLTHKASDLYITVGAPMLYRVDGELRAQGEVLNMAQVTTLLNAMMDDARQAEFKQTREANFAVVRDSGRFR
VSAFFQRELPGAVIRRIETQIPTFEELNLPAVLQNLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNQHRTGHILTVEDPI
EFVHEHQRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQREQFLLDLSLNLKGVIGQQLLRDKHGQGRHGVFEILLNSPRIADLIRRGELHELKTTMARSQEIGMQTFDQALY
QLVVDDKINEQDALHSADSANDLRLMLKTKRGTHYSSGALHNVQIDME

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=761087 OOK74_RS01785 WP_274723623.1 365880..366986(+) (pilU) [Vibrio sp. gvc]
ATGGATTTAAATCAATATCTGGATGGTATGCTGACGCATAAAGCATCGGATCTGTACATTACCGTGGGCGCACCGATGTT
ATATCGGGTTGATGGCGAACTGCGCGCACAAGGTGAAGTGCTCAATATGGCACAGGTGACCACGTTGCTGAATGCGATGA
TGGATGACGCGCGGCAAGCCGAATTTAAGCAGACTCGTGAAGCGAACTTTGCCGTGGTACGCGACAGTGGTCGATTTCGG
GTCAGTGCTTTTTTTCAGCGTGAGCTGCCGGGGGCGGTGATCCGCCGTATTGAAACCCAGATCCCGACTTTTGAAGAGCT
TAACTTGCCGGCGGTGCTACAAAATTTGGCGATTGCCAAACGCGGTTTAGTGCTGGTGGTTGGGGCCACGGGCTCAGGTA
AATCGACCACTATGGCGGCGATGACCGGTTATCGGAATCAGCATCGTACTGGGCACATTTTAACCGTGGAAGATCCGATT
GAGTTTGTGCATGAGCATCAGCGCTGCATCGTCACTCAACGTGAAGTGGGGCTGGATACCGAAAGTTATGAAGTGGCGCT
GAAAAACTCGCTACGCCAAGCGCCAGATATGATCTTGATTGGTGAAATCCGTAGCCGAGAGACGATGGAATACGCGATGA
CTTTTGCCGAAACCGGTCATTTGTGTATGGCCACTTTGCACGCCAACAATGCCAACCAAGCCTTAGAGCGCATTTTGCAT
TTGGTGCCTAAAGAGCAGCGCGAGCAATTTTTACTCGACTTATCGCTCAATCTCAAAGGGGTGATTGGTCAACAGTTGCT
GCGTGATAAGCACGGCCAAGGCCGGCATGGGGTGTTTGAAATTTTGCTCAATAGTCCGCGCATTGCTGATTTAATTCGGC
GTGGTGAGCTGCATGAGCTGAAAACTACTATGGCGCGCTCGCAAGAAATTGGTATGCAGACCTTTGATCAAGCGCTGTAT
CAATTGGTGGTGGATGACAAAATCAATGAGCAAGATGCGCTACACAGCGCTGATTCGGCCAACGATCTGCGTTTGATGCT
CAAAACCAAGCGTGGCACTCACTACTCAAGTGGTGCTCTGCACAATGTGCAAATTGATATGGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

93.207

100

0.932
  pilU Pseudomonas stutzeri DSM 10701

57.429

95.109

0.546
  pilU Acinetobacter baylyi ADP1

54.416

95.38

0.519
  pilT Acinetobacter baylyi ADP1

42.229

92.663

0.391
  pilT Legionella pneumophila strain ERS1305867

41.888

92.12

0.386
  pilT Legionella pneumophila strain Lp02

41.888

92.12

0.386
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.484

84.239

0.383
  pilT Vibrio cholerae strain A1552

41.617

90.761

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.617

90.761

0.378
  pilT Pseudomonas aeruginosa PAK

41.003

92.12

0.378
  pilT Acinetobacter baumannii strain A118

40.708

92.12

0.375
  pilT Acinetobacter baumannii D1279779

40.708

92.12

0.375
  pilT Acinetobacter nosocomialis M2

40.708

92.12

0.375
  pilT Pseudomonas stutzeri DSM 10701

40.413

92.12

0.372