Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   H7R73_RS02545 Genome accession   NZ_AP021917
Coordinates   518405..519439 (-) Length   344 a.a.
NCBI ID   WP_010673050.1    Uniprot ID   A0A081LS93
Organism   Aeromonas sp. WP2-W18-CRE-05     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 513405..524439
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7R73_RS02525 (WP2W18C05_04870) - 514220..514930 (-) 711 WP_103260858.1 methyltransferase -
  H7R73_RS02530 (WP2W18C05_04880) srmB 515058..516278 (+) 1221 WP_041210934.1 ATP-dependent RNA helicase SrmB -
  H7R73_RS02535 (WP2W18C05_04890) yaaA 516466..517239 (-) 774 WP_103260857.1 peroxide stress protein YaaA -
  H7R73_RS02540 (WP2W18C05_04900) pilU 517272..518381 (-) 1110 WP_182951465.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  H7R73_RS02545 (WP2W18C05_04910) pilT 518405..519439 (-) 1035 WP_010673050.1 type IVa pilus ATPase TapT Machinery gene
  H7R73_RS02550 (WP2W18C05_04920) - 519479..520180 (+) 702 WP_042864460.1 YggS family pyridoxal phosphate-dependent enzyme -
  H7R73_RS02555 (WP2W18C05_04930) proC 520302..521126 (+) 825 WP_103260856.1 pyrroline-5-carboxylate reductase -
  H7R73_RS02560 (WP2W18C05_04940) - 521150..521701 (+) 552 WP_103260855.1 YggT family protein -
  H7R73_RS02565 (WP2W18C05_04950) yggU 521701..522000 (+) 300 WP_041210939.1 DUF167 family protein YggU -
  H7R73_RS02570 (WP2W18C05_04960) - 522019..522438 (+) 420 WP_049636470.1 DUF4426 domain-containing protein -
  H7R73_RS02575 (WP2W18C05_04970) - 522514..523344 (-) 831 WP_103260854.1 CPBP family intramembrane glutamic endopeptidase -
  H7R73_RS02580 (WP2W18C05_04980) - 523506..523796 (-) 291 WP_039039963.1 hypothetical protein -
  H7R73_RS02585 (WP2W18C05_04990) - 523846..524220 (-) 375 WP_171864935.1 YacL family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38189.90 Da        Isoelectric Point: 6.7436

>NTDB_id=75835 H7R73_RS02545 WP_010673050.1 518405..519439(-) (pilT) [Aeromonas sp. WP2-W18-CRE-05]
MDITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINLPALDHREVHALIYDIMNDHQRKELEENFEVDFSFEVPGMAR
FRVNAFQQARGSGAVFRTIPSTVLTLDDLGAPEIFRKIAEYPRGLVLVTGPTGSGKSTTLAAMVDYINDNFHHHILTIED
PIEFVHENKRCLVNQREVHRDTKSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSEKDMVRSMLSESLRAVISQTLLKRIGGGRVAAHEIMLGIPAVRNLIREDKVAQLYSVIQTGMTHGMQTMDQSL
KQLVNRGVVASLDAKAKAVDPNTI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=75835 H7R73_RS02545 WP_010673050.1 518405..519439(-) (pilT) [Aeromonas sp. WP2-W18-CRE-05]
ATGGATATCACAGAGTTATTGGCATTCAGTGTAAAGCATAAGGCCTCGGATCTACACCTCTCGGCCGGGGTCCCCCCGAT
GATCAGGGTTGATGGGGAGGTTCGCAAGATCAATTTGCCCGCCCTGGATCACCGGGAAGTCCATGCCCTCATTTACGACA
TCATGAATGACCACCAGCGCAAGGAGCTGGAGGAGAACTTCGAGGTGGACTTCTCCTTCGAAGTGCCCGGCATGGCACGC
TTTCGGGTCAACGCCTTCCAGCAGGCGCGCGGCTCGGGCGCGGTGTTTCGTACCATCCCGAGTACCGTTCTGACCCTGGA
CGATCTGGGGGCGCCGGAGATATTTCGCAAGATAGCGGAGTACCCTCGCGGCCTGGTGCTGGTGACTGGGCCCACCGGTT
CGGGCAAATCCACCACCCTGGCCGCCATGGTCGATTACATCAATGACAACTTCCACCACCACATCCTCACCATCGAGGAC
CCCATCGAATTCGTGCACGAGAACAAGCGCTGCCTGGTGAACCAGCGGGAAGTACACCGCGACACCAAGAGCTTCAGCAA
CGCCCTGCGCTCGGCCCTGCGGGAAGACCCGGATATCATCCTGGTAGGTGAAATGCGGGACCTCGAAACCATCCGTCTCG
CCATGACCGCCGCCGAAACCGGTCACCTGGTGTTTGGCACCCTGCACACCTCCTCAGCTGCCAAGACCATCGACCGTATC
ATCGACGTCTTCCCCGGCTCGGAGAAGGACATGGTGCGATCCATGCTCTCCGAATCCCTGCGGGCGGTCATCTCCCAGAC
CCTGCTCAAGCGTATCGGTGGCGGCCGGGTGGCGGCCCACGAGATCATGCTGGGAATCCCGGCGGTGCGAAACCTCATCC
GGGAAGACAAGGTGGCCCAGCTCTATTCCGTCATCCAGACCGGGATGACCCACGGCATGCAGACCATGGACCAGAGCCTC
AAGCAGCTGGTCAACCGGGGGGTCGTCGCCTCCCTTGATGCCAAGGCCAAGGCGGTCGATCCCAATACCATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A081LS93

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

77.907

100

0.779
  pilT Pseudomonas stutzeri DSM 10701

78.299

99.128

0.776
  pilT Acinetobacter baumannii D1279779

77.035

100

0.77
  pilT Acinetobacter baumannii strain A118

77.035

100

0.77
  pilT Acinetobacter nosocomialis M2

77.035

100

0.77
  pilT Pseudomonas aeruginosa PAK

76.833

99.128

0.762
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

76.261

97.965

0.747
  pilT Vibrio cholerae strain A1552

76.261

97.965

0.747
  pilT Legionella pneumophila strain Lp02

71.471

98.837

0.706
  pilT Legionella pneumophila strain ERS1305867

71.471

98.837

0.706
  pilT Neisseria meningitidis 8013

69.027

98.547

0.68
  pilT Neisseria gonorrhoeae MS11

68.732

98.547

0.677
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.924

99.419

0.526
  pilU Vibrio cholerae strain A1552

43.188

100

0.433
  pilU Pseudomonas stutzeri DSM 10701

41.159

100

0.413
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407

Multiple sequence alignment