Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   H7R73_RS02540 Genome accession   NZ_AP021917
Coordinates   517272..518381 (-) Length   369 a.a.
NCBI ID   WP_182951465.1    Uniprot ID   -
Organism   Aeromonas sp. WP2-W18-CRE-05     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 512272..523381
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7R73_RS02515 (WP2W18C05_04850) - 512405..512632 (+) 228 WP_039039956.1 hypothetical protein -
  H7R73_RS02520 (WP2W18C05_04860) brnQ 512722..514029 (-) 1308 WP_103261190.1 branched-chain amino acid transport system II carrier protein -
  H7R73_RS02525 (WP2W18C05_04870) - 514220..514930 (-) 711 WP_103260858.1 methyltransferase -
  H7R73_RS02530 (WP2W18C05_04880) srmB 515058..516278 (+) 1221 WP_041210934.1 ATP-dependent RNA helicase SrmB -
  H7R73_RS02535 (WP2W18C05_04890) yaaA 516466..517239 (-) 774 WP_103260857.1 peroxide stress protein YaaA -
  H7R73_RS02540 (WP2W18C05_04900) pilU 517272..518381 (-) 1110 WP_182951465.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  H7R73_RS02545 (WP2W18C05_04910) pilT 518405..519439 (-) 1035 WP_010673050.1 type IVa pilus ATPase TapT Machinery gene
  H7R73_RS02550 (WP2W18C05_04920) - 519479..520180 (+) 702 WP_042864460.1 YggS family pyridoxal phosphate-dependent enzyme -
  H7R73_RS02555 (WP2W18C05_04930) proC 520302..521126 (+) 825 WP_103260856.1 pyrroline-5-carboxylate reductase -
  H7R73_RS02560 (WP2W18C05_04940) - 521150..521701 (+) 552 WP_103260855.1 YggT family protein -
  H7R73_RS02565 (WP2W18C05_04950) yggU 521701..522000 (+) 300 WP_041210939.1 DUF167 family protein YggU -
  H7R73_RS02570 (WP2W18C05_04960) - 522019..522438 (+) 420 WP_049636470.1 DUF4426 domain-containing protein -
  H7R73_RS02575 (WP2W18C05_04970) - 522514..523344 (-) 831 WP_103260854.1 CPBP family intramembrane glutamic endopeptidase -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40843.00 Da        Isoelectric Point: 6.4405

>NTDB_id=75834 H7R73_RS02540 WP_182951465.1 517272..518381(-) (pilU) [Aeromonas sp. WP2-W18-CRE-05]
MNMDVLLAALVEQKGSDLFITVDAPPTLKVNGRLVSLGPAPLDKTTALALIRESLGETHFERFLHTREANYAIQRGSLGR
FRVSAFWQQDMPGMVLRRIETRIPTFDELVLPPILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGFRNQHGDGHILTVED
PVEFVHQHDRCLVTQREVGIDTASFDVALKNSLRQAPDVILIGEIRSQETMEFAIQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSAHGQRRVAAFEILLNTPLVTDIIRKGEMHRLKEVMTKSGELGMQTFDQA
LFTLFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGAGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=75834 H7R73_RS02540 WP_182951465.1 517272..518381(-) (pilU) [Aeromonas sp. WP2-W18-CRE-05]
ATGAACATGGATGTTCTGCTCGCCGCACTGGTTGAACAAAAGGGCTCGGATCTCTTCATCACGGTCGATGCCCCCCCGAC
CCTCAAGGTCAACGGGCGTCTGGTGTCGCTCGGGCCGGCTCCCCTCGACAAGACGACGGCGCTGGCGTTGATCAGGGAGA
GCCTGGGCGAGACCCACTTCGAGCGCTTCCTGCACACCCGCGAGGCCAACTACGCCATCCAGCGAGGCTCGCTGGGGCGC
TTTCGGGTCAGCGCCTTCTGGCAACAGGATATGCCGGGCATGGTGCTGCGCCGCATCGAGACCCGCATCCCCACCTTTGA
CGAGCTGGTGCTGCCCCCCATCCTGCAGGAGGTCGCCATGGCCAAACGCGGGCTGGTGCTCTTCGTCGGCGCCACTGGCG
CCGGCAAGTCCACCACCCAGGCGGCCATGATAGGCTTTCGCAACCAGCACGGTGACGGCCACATCCTGACGGTGGAGGAT
CCGGTGGAGTTCGTCCATCAGCACGATCGCTGTCTGGTGACCCAGCGGGAGGTGGGGATCGACACCGCATCCTTCGATGT
CGCCCTCAAAAACTCCCTGCGCCAGGCCCCGGATGTCATCCTCATCGGTGAAATCCGCTCCCAGGAGACCATGGAGTTCG
CCATCCAGTTCGCCGAGACGGGACACCTCTGCCTCGCCACCTTGCACGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTGCATCTGGTCCCCCAGGAGAAGCACCGCCAGTTCCTGTTCGATCTCTCCTTCAACCTGCGCGCCATCGTCGCCCAGCA
GCTGCTCCCCTCCGCTCACGGGCAGCGCCGGGTCGCCGCCTTCGAGATCCTGCTCAACACCCCGCTCGTCACCGACATCA
TTCGCAAGGGAGAGATGCACAGACTCAAGGAGGTGATGACCAAATCCGGCGAGCTTGGCATGCAGACCTTCGATCAGGCG
CTGTTCACCCTGTTCTGCGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCCGCCAACGACCTGCGACT
GCTCATCAAGCTGTCGGGTCGCGAGCAGCTGGGCGCAGGCACTCTCGACAACGTGACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.326

99.729

0.602
  pilU Pseudomonas stutzeri DSM 10701

58.974

95.122

0.561
  pilU Acinetobacter baylyi ADP1

57.79

95.664

0.553
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.857

91.057

0.39
  pilT Legionella pneumophila strain Lp02

40.816

92.954

0.379
  pilT Legionella pneumophila strain ERS1305867

40.816

92.954

0.379

Multiple sequence alignment