Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PHLH3_RS02025 Genome accession   NZ_AP021905
Coordinates   413812..414846 (+) Length   344 a.a.
NCBI ID   WP_111262598.1    Uniprot ID   -
Organism   Pseudomonas sp. Pc102     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 408812..419846
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PHLH3_RS02000 (PHLH8_04010) - 409952..410248 (-) 297 WP_213627928.1 DUF167 domain-containing protein -
  PHLH3_RS02005 (PHLH8_04020) - 410248..410841 (-) 594 WP_173175076.1 YggT family protein -
  PHLH3_RS02010 (PHLH8_04030) - 410992..412209 (-) 1218 WP_213627930.1 hypothetical protein -
  PHLH3_RS02015 (PHLH8_04040) proC 412215..413033 (-) 819 WP_213627932.1 pyrroline-5-carboxylate reductase -
  PHLH3_RS02020 (PHLH8_04050) - 413048..413743 (-) 696 WP_213627934.1 YggS family pyridoxal phosphate-dependent enzyme -
  PHLH3_RS02025 (PHLH8_04060) pilT 413812..414846 (+) 1035 WP_111262598.1 type IV pilus twitching motility protein PilT Machinery gene
  PHLH3_RS02030 (PHLH8_04070) pilU 414976..416121 (+) 1146 WP_111262597.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PHLH3_RS02035 (PHLH8_04080) - 416054..417547 (-) 1494 WP_213627936.1 UdgX family uracil-DNA binding protein -
  PHLH3_RS02040 (PHLH8_04090) - 417547..418761 (-) 1215 WP_213627938.1 putative DNA modification/repair radical SAM protein -
  PHLH3_RS02045 (PHLH8_04100) - 418863..419258 (+) 396 WP_173175068.1 TM2 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37831.54 Da        Isoelectric Point: 6.8360

>NTDB_id=75738 PHLH3_RS02025 WP_111262598.1 413812..414846(+) (pilT) [Pseudomonas sp. Pc102]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPAMDHKQVHALIYDIMNDKQRKDYEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMEDLGMGEIFKKISDVPRGLVLVTGPTGSGKSTTLAAMLDYLNGTKYHHILTVED
PIEFVHESKKCLINQREVHRDTLGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMVRSMLSESLQSVISQTLLKKIGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGGGLGMQTLDSCL
KGLVAKGLVSRESAREKAKSPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=75738 PHLH3_RS02025 WP_111262598.1 413812..414846(+) (pilT) [Pseudomonas sp. Pc102]
ATGGATATTACTGAGCTGCTCGCCTTCAGCGCGAAGCAGGGCGCATCCGACCTGCATCTCTCTGCCGGCCTGCCGCCGAT
GATCCGCGTCGATGGTGACGTGCGCCGCATCAACCTGCCGGCCATGGACCACAAGCAGGTGCACGCGCTGATCTACGACA
TCATGAATGACAAGCAGCGCAAGGACTACGAGGAGTTCCTCGAGACCGACTTCTCCTTCGAAGTCCCTGGCGTGGCGCGT
TTCCGGGTCAACGCCTTCAACCAGAACCGTGGCGCCGGCGCCGTGTTCCGTACCATTCCCTCGAAGGTACTGAGCATGGA
GGACCTGGGCATGGGCGAGATCTTCAAGAAGATCTCCGACGTGCCGCGCGGGCTGGTGCTGGTGACCGGGCCGACCGGTT
CGGGCAAGTCCACCACCCTGGCGGCGATGCTCGACTACCTCAACGGCACCAAGTACCACCACATCCTCACGGTCGAGGAC
CCCATCGAATTCGTCCACGAATCGAAGAAGTGCCTGATCAACCAGCGCGAGGTGCACCGCGACACCCTCGGCTTCAACGA
AGCCCTGCGCTCGGCCCTGCGGGAAGACCCGGACATCATCCTGGTGGGCGAGATGCGCGACCTGGAGACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACCACCTCCGCCGCCAAGACCATCGACCGGGTG
GTCGACGTGTTCCCGGCCGAGGAGAAGTCGATGGTCCGCTCGATGCTTTCCGAATCCCTCCAGTCGGTCATTTCCCAGAC
CCTGCTGAAGAAGATCGGCGGCGGTCGCGTGGCGGCCCATGAAATCATGATCGGCACCCCGGCCATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCCGCCATCCAGACCGGCGGTGGCCTCGGCATGCAGACCCTGGACAGCTGCCTC
AAGGGGCTGGTGGCCAAGGGCCTGGTGAGCCGCGAAAGCGCTCGCGAGAAGGCCAAGAGCCCGGAAAACTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

94.477

100

0.945
  pilT Pseudomonas aeruginosa PAK

94.186

100

0.942
  pilT Acinetobacter baumannii D1279779

85.756

100

0.858
  pilT Acinetobacter baumannii strain A118

85.756

100

0.858
  pilT Acinetobacter nosocomialis M2

85.465

100

0.855
  pilT Acinetobacter baylyi ADP1

83.721

100

0.837
  pilT Legionella pneumophila strain Lp02

75.444

98.256

0.741
  pilT Legionella pneumophila strain ERS1305867

75.444

98.256

0.741
  pilT Neisseria meningitidis 8013

68.116

100

0.683
  pilT Neisseria gonorrhoeae MS11

67.826

100

0.68
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.139

97.965

0.677
  pilT Vibrio cholerae strain A1552

69.139

97.965

0.677
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

100

0.515
  pilU Vibrio cholerae strain A1552

39.71

100

0.398
  pilU Acinetobacter baylyi ADP1

39.595

100

0.398
  pilU Pseudomonas stutzeri DSM 10701

39.13

100

0.392

Multiple sequence alignment