Detailed information NTG0075739

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PHLH3_RS02030
• Genome accession: NZ_AP021905
• Coordinates: 414976..416121 (+)
• Length: 381 a.a.
• NCBI ID: WP_111262597.1
• Uniprot ID: -
• Organism: Pseudomonas sp. Pc102
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 409976..421121

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PHLH3_RS02005 (PHLH8_04020)	-	410248..410841 (-)	594	WP_173175076.1	YggT family protein	-
PHLH3_RS02010 (PHLH8_04030)	-	410992..412209 (-)	1218	WP_213627930.1	hypothetical protein	-
PHLH3_RS02015 (PHLH8_04040)	proC	412215..413033 (-)	819	WP_213627932.1	pyrroline-5-carboxylate reductase	-
PHLH3_RS02020 (PHLH8_04050)	-	413048..413743 (-)	696	WP_213627934.1	YggS family pyridoxal phosphate-dependent enzyme	-
PHLH3_RS02025 (PHLH8_04060)	pilT	413812..414846 (+)	1035	WP_111262598.1	type IV pilus twitching motility protein PilT	Machinery gene
PHLH3_RS02030 (PHLH8_04070)	pilU	414976..416121 (+)	1146	WP_111262597.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
PHLH3_RS02035 (PHLH8_04080)	-	416054..417547 (-)	1494	WP_213627936.1	UdgX family uracil-DNA binding protein	-
PHLH3_RS02040 (PHLH8_04090)	-	417547..418761 (-)	1215	WP_213627938.1	putative DNA modification/repair radical SAM protein	-
PHLH3_RS02045 (PHLH8_04100)	-	418863..419258 (+)	396	WP_173175068.1	TM2 domain-containing protein	-
PHLH3_RS02050 (PHLH8_04110)	-	419371..420750 (+)	1380	WP_213627940.1	pyridoxal-phosphate dependent enzyme	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42490.78 Da
• Isoelectric Point: 6.5931

>NTDB_id=75739 PHLH3_RS02030 WP_111262597.1 414976..416121(+) (pilU) [Pseudomonas sp. Pc102]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPVTKNAMSPEQTRETVLAVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLDDLKLPEILKKLALTKRGLVLFVGATGTGKSTSLAAMIGYRNKNSSGHIISIED
PIEYIHQHQNCIVTQREVGIDTESFEVALKNTLRQAPDVILIGEVRTRETMDHAVAFAETGHLCLATLHANNANQALDRI
INFFPADRQQQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYTLYSQGEITYEDALLYADSANDLRLMIKLGSETDGDHLTSMSGGLSLEVSEEDPGRRFR

Nucleotide

• Length: 1146 bp

>NTDB_id=75739 PHLH3_RS02030 WP_111262597.1 414976..416121(+) (pilU) [Pseudomonas sp. Pc102]
ATGGAATTCGAGAAACTGTTGCGCCTGATGGTCGAGAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTCCCCCCGTC
GATGAAGGTCAACGGCAAGATCATGCCCGTGACCAAGAACGCCATGTCCCCGGAGCAGACCCGCGAGACCGTCCTCGCGG
TGATGAACGAGCAGCAGCGCCGCGACTTCGCCGAGAACCACGAGTGCAACTTCGCCATCAGCGCCCGGGGCATCGGTCGT
TTCCGCGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTGCTGCGCCGCATCGAGACCAACATCCCCACCCTGGA
TGACCTCAAGCTGCCGGAGATCCTCAAGAAGCTGGCGCTGACCAAGCGTGGCCTGGTGCTGTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACAGCAGCGGCCACATCATCTCCATCGAAGAC
CCGATCGAATACATCCACCAGCACCAGAACTGCATCGTCACCCAGCGCGAGGTGGGCATCGACACCGAGTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTCATCGGCGAGGTGCGGACCCGCGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGGATC
ATCAACTTCTTCCCCGCCGACCGGCAGCAGCAGGTGTGGATGGACCTGTCCCTCAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCGGACGGCAAGGGCCGCCGCGCGGTGATCGAGGTGCTGATCAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTCCACGAGCTCAAGCCGCTGATGAAGCGTTCCACCGAGCAGGGTATGCAGACCTTCGACCAGGCG
TTGTACACCCTCTACTCCCAGGGCGAGATCACCTACGAAGACGCACTGCTCTACGCCGACTCGGCGAACGACCTGCGCCT
GATGATCAAGCTCGGTTCCGAGACCGACGGCGACCACCTCACGAGCATGAGCGGTGGCCTGTCGCTGGAAGTATCGGAGG
AAGACCCGGGCCGTCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (110-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	91.601	100	0.916
pilU	Acinetobacter baylyi ADP1	64.426	93.701	0.604
pilU	Vibrio cholerae strain A1552	58.857	91.864	0.541
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.155	88.189	0.381