Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KI243_RS30740 Genome accession   NZ_AP021904
Coordinates   6919018..6920052 (-) Length   344 a.a.
NCBI ID   WP_017124853.1    Uniprot ID   A0A7Y7XYC1
Organism   Pseudomonas sp. Ost2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6914018..6925052
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KI243_RS30720 (PHLH7_60540) - 6914887..6916158 (+) 1272 WP_212810243.1 dihydroorotase -
  KI243_RS30725 (PHLH7_60550) - 6916324..6916743 (-) 420 WP_212810244.1 TM2 domain-containing protein -
  KI243_RS30730 (PHLH7_60560) - 6916995..6917654 (+) 660 WP_177087054.1 C40 family peptidase -
  KI243_RS30735 (PHLH7_60570) pilU 6917800..6918906 (-) 1107 WP_017124852.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KI243_RS30740 (PHLH7_60580) pilT 6919018..6920052 (-) 1035 WP_017124853.1 type IV pilus twitching motility protein PilT Machinery gene
  KI243_RS30745 (PHLH7_60590) - 6920109..6920795 (+) 687 WP_212810245.1 YggS family pyridoxal phosphate-dependent enzyme -
  KI243_RS30750 (PHLH7_60600) proC 6920829..6921647 (+) 819 WP_212810246.1 pyrroline-5-carboxylate reductase -
  KI243_RS30755 (PHLH7_60610) - 6921666..6922256 (+) 591 WP_017124856.1 YggT family protein -
  KI243_RS30760 (PHLH7_60620) - 6922461..6923600 (+) 1140 WP_017124857.1 homoserine O-acetyltransferase -
  KI243_RS30765 (PHLH7_60630) metW 6923608..6924228 (+) 621 WP_017124858.1 methionine biosynthesis protein MetW -
  KI243_RS30770 (PHLH7_60640) - 6924255..6924686 (+) 432 WP_177087058.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38071.77 Da        Isoelectric Point: 6.8889

>NTDB_id=75729 KI243_RS30740 WP_017124853.1 6919018..6920052(-) (pilT) [Pseudomonas sp. Ost2]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHDLIYDIMNDRQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMNDLGMGEVFRKITEVPRGLVLVTGPTGSGKSTTLAAMIDYLNNNKHHHILTIED
PIEFVHESKKCLVNQREVHRDTQSFSAALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMVRSMLSESLQAVISQSLLKKVGGGRVAAHEIMLGTPAIRNLIREDKVAQMYSSIQTGGSLGMQTLDMCL
KDLLAKGLITRDNAREKAKIPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=75729 KI243_RS30740 WP_017124853.1 6919018..6920052(-) (pilT) [Pseudomonas sp. Ost2]
ATGGATATCACCGAGCTGCTGGCCTTTAGCGCCAAACAGGGCGCGTCGGACTTGCACCTCTCGGCCGGGCTGCCGCCGAT
GATCCGGGTCGATGGTGACGTGCGCCGGATCAACCTGCCGGCGCTGGACCACAAGCAGGTCCACGACCTGATCTACGACA
TCATGAATGACCGCCAGCGCAAGGATTTCGAGGAGTTCCTGGAAACCGACTTCTCCTTCGAGGTGCCGGGCGTGGCGCGT
TTCCGGGTCAACGCCTTCAACCAGAACCGTGGTGCGGGCGCGGTGTTCCGGACCATTCCGTCGAAAGTCCTGACCATGAA
CGACCTGGGCATGGGCGAGGTGTTCCGCAAGATTACCGAGGTTCCTCGTGGCCTGGTGCTAGTGACTGGCCCAACCGGTT
CGGGTAAATCGACCACCCTGGCGGCGATGATCGACTACCTGAACAACAACAAGCACCACCATATCCTGACCATCGAGGAC
CCGATCGAATTCGTCCACGAGTCGAAGAAGTGCCTGGTCAACCAGCGTGAAGTGCACCGCGACACGCAGAGTTTCTCCGC
CGCCCTGCGCTCGGCCCTGCGGGAAGACCCGGACGTGATCCTGGTGGGCGAGATGCGCGACCTGGAAACCATTCGCCTGG
CACTGACCGCCGCCGAAACCGGCCACCTGGTGTTCGGTACGCTGCACACCACCTCCGCGGCGAAGACCATCGACCGGGTG
GTCGACGTGTTCCCGGCGGAAGAGAAATCCATGGTCCGCTCGATGCTCTCCGAATCCCTGCAGGCGGTGATTTCCCAGAG
CCTGCTGAAAAAGGTCGGCGGCGGGCGGGTCGCGGCCCATGAAATCATGCTCGGCACCCCGGCGATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTACTCGTCGATCCAGACTGGCGGCTCCCTGGGCATGCAGACCCTGGACATGTGCCTC
AAGGATCTGTTGGCCAAGGGCCTGATCACCCGCGATAACGCCCGGGAGAAGGCAAAGATTCCCGACAACTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7Y7XYC1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

93.605

100

0.936
  pilT Pseudomonas aeruginosa PAK

91.279

100

0.913
  pilT Acinetobacter baumannii D1279779

81.105

100

0.811
  pilT Acinetobacter baumannii strain A118

81.105

100

0.811
  pilT Acinetobacter nosocomialis M2

80.814

100

0.808
  pilT Acinetobacter baylyi ADP1

80.233

100

0.802
  pilT Legionella pneumophila strain Lp02

74.852

98.256

0.735
  pilT Legionella pneumophila strain ERS1305867

74.852

98.256

0.735
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.118

98.837

0.683
  pilT Vibrio cholerae strain A1552

69.118

98.837

0.683
  pilT Neisseria meningitidis 8013

67.647

98.837

0.669
  pilT Neisseria gonorrhoeae MS11

67.353

98.837

0.666
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.913

100

0.541
  pilU Pseudomonas stutzeri DSM 10701

41.791

97.384

0.407
  pilU Vibrio cholerae strain A1552

41.003

98.547

0.404
  pilU Acinetobacter baylyi ADP1

39.884

100

0.401

Multiple sequence alignment