Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   KI243_RS30735 Genome accession   NZ_AP021904
Coordinates   6917800..6918906 (-) Length   368 a.a.
NCBI ID   WP_017124852.1    Uniprot ID   A0A7Y7XY93
Organism   Pseudomonas sp. Ost2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6912800..6923906
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KI243_RS30705 (PHLH7_60510) ruvX 6912847..6913287 (+) 441 WP_103032754.1 Holliday junction resolvase RuvX -
  KI243_RS30710 (PHLH7_60520) pyrR 6913356..6913862 (+) 507 WP_212810242.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  KI243_RS30715 (PHLH7_60530) - 6913886..6914890 (+) 1005 WP_103032755.1 aspartate carbamoyltransferase catalytic subunit -
  KI243_RS30720 (PHLH7_60540) - 6914887..6916158 (+) 1272 WP_212810243.1 dihydroorotase -
  KI243_RS30725 (PHLH7_60550) - 6916324..6916743 (-) 420 WP_212810244.1 TM2 domain-containing protein -
  KI243_RS30730 (PHLH7_60560) - 6916995..6917654 (+) 660 WP_177087054.1 C40 family peptidase -
  KI243_RS30735 (PHLH7_60570) pilU 6917800..6918906 (-) 1107 WP_017124852.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KI243_RS30740 (PHLH7_60580) pilT 6919018..6920052 (-) 1035 WP_017124853.1 type IV pilus twitching motility protein PilT Machinery gene
  KI243_RS30745 (PHLH7_60590) - 6920109..6920795 (+) 687 WP_212810245.1 YggS family pyridoxal phosphate-dependent enzyme -
  KI243_RS30750 (PHLH7_60600) proC 6920829..6921647 (+) 819 WP_212810246.1 pyrroline-5-carboxylate reductase -
  KI243_RS30755 (PHLH7_60610) - 6921666..6922256 (+) 591 WP_017124856.1 YggT family protein -
  KI243_RS30760 (PHLH7_60620) - 6922461..6923600 (+) 1140 WP_017124857.1 homoserine O-acetyltransferase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41070.39 Da        Isoelectric Point: 6.6379

>NTDB_id=75728 KI243_RS30735 WP_017124852.1 6917800..6918906(-) (pilU) [Pseudomonas sp. Ost2]
MEFEKLLRLMVEKGGSDLFITPGLPPSLKVNGQILPVTRNPLTQEQTHDAVFGLMSERQKLDFTHLHECNFAIHPKGIGR
FRVSAFYQRNQPGMVLRRIETQIPQLADLKLPEVIEQLALLKRGLVMFVGATGTGKSTSLAAMIGHRNQQGSGHIVTVED
PIEFIHEHQNCIITQREVGIDTDSYEVALKNTLRQAPDLIMIGEVRTRETMGHALAFAETGHLCLATLHAPNADQALERI
INFFPVDRHPQIWMELSLNLRAIVAQQLIPTRDGTGRRAVVEVLLNTPLMADLIRKGEIKELKSLMKRSTEQGMQTLDQA
LYVLYQEGAISYRDALAYADSANDLRLMIKLEKGAGSETSADLSLNPE

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=75728 KI243_RS30735 WP_017124852.1 6917800..6918906(-) (pilU) [Pseudomonas sp. Ost2]
ATGGAGTTCGAGAAACTGCTGCGCCTGATGGTGGAGAAGGGCGGTTCCGACCTGTTTATCACCCCGGGACTGCCGCCGTC
GCTGAAGGTCAACGGCCAGATCCTGCCGGTGACCCGCAACCCGCTGACCCAGGAACAGACCCACGACGCGGTGTTCGGAC
TGATGAGCGAGCGCCAGAAGCTCGACTTCACCCACCTGCACGAGTGCAATTTCGCCATCCATCCCAAGGGCATCGGCCGC
TTCCGCGTGAGCGCCTTCTACCAGCGCAACCAACCGGGCATGGTGCTGCGGCGGATCGAAACGCAGATTCCGCAACTGGC
CGACCTGAAGCTGCCGGAGGTCATCGAACAACTGGCGCTGCTCAAGCGCGGGCTGGTGATGTTCGTCGGCGCCACCGGCA
CTGGCAAGTCGACGTCCCTGGCGGCGATGATCGGTCACCGCAACCAGCAGGGCAGCGGTCATATCGTCACCGTCGAAGAC
CCCATCGAATTCATCCACGAACACCAGAACTGCATCATCACCCAGCGCGAAGTGGGCATCGATACCGATTCCTACGAGGT
GGCGCTGAAAAATACCCTGCGCCAGGCACCGGACCTGATCATGATCGGTGAAGTCCGCACCCGCGAAACCATGGGCCATG
CCCTGGCCTTCGCGGAAACCGGGCATCTGTGCCTGGCGACCCTGCACGCGCCGAATGCCGACCAGGCGCTGGAGCGGATC
ATCAACTTCTTCCCGGTGGACCGGCATCCGCAGATCTGGATGGAACTGTCCCTCAACCTGCGCGCCATCGTCGCCCAGCA
ACTGATTCCGACGCGGGACGGCACCGGCAGGCGGGCGGTGGTGGAAGTGCTGTTGAACACGCCGCTGATGGCCGACCTGA
TCCGCAAGGGCGAGATCAAGGAGCTCAAGTCGCTGATGAAACGCTCGACCGAACAGGGCATGCAGACCCTGGACCAGGCG
TTGTACGTGTTGTACCAGGAGGGCGCGATCAGCTACCGCGATGCGCTGGCCTATGCGGATTCGGCCAATGACCTGCGGTT
GATGATCAAGCTGGAGAAGGGCGCGGGTAGCGAGACATCGGCTGACTTGAGCCTGAACCCGGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(108-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7Y7XY93

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

75.414

98.37

0.742
  pilU Acinetobacter baylyi ADP1

62.465

97.011

0.606
  pilU Vibrio cholerae strain A1552

56.78

96.196

0.546
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.298

92.12

0.38

Multiple sequence alignment