Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OOT43_RS01245 Genome accession   NZ_CP110921
Coordinates   243946..244980 (-) Length   344 a.a.
NCBI ID   WP_266022834.1    Uniprot ID   -
Organism   Methylococcus mesophilus strain 16-5     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 238946..249980
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OOT43_RS01220 (OOT43_01220) - 239489..240271 (+) 783 WP_266022829.1 succinate dehydrogenase iron-sulfur subunit -
  OOT43_RS01225 (OOT43_01225) - 240268..240525 (+) 258 WP_266022830.1 succinate dehydrogenase assembly factor 2 -
  OOT43_RS01230 (OOT43_01230) - 240519..240941 (+) 423 WP_266022831.1 protein YgfX -
  OOT43_RS01235 (OOT43_01235) - 240952..242742 (+) 1791 WP_266022832.1 VWA domain-containing protein -
  OOT43_RS01240 (OOT43_01240) pilU 242807..243931 (-) 1125 WP_266022833.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OOT43_RS01245 (OOT43_01245) pilT 243946..244980 (-) 1035 WP_266022834.1 type IV pilus twitching motility protein PilT Machinery gene
  OOT43_RS01250 (OOT43_01250) - 245034..245723 (+) 690 WP_266022835.1 YggS family pyridoxal phosphate-dependent enzyme -
  OOT43_RS01255 (OOT43_01255) proC 245791..246618 (+) 828 WP_266022836.1 pyrroline-5-carboxylate reductase -
  OOT43_RS01260 (OOT43_01260) - 246618..247196 (+) 579 WP_266022837.1 YggT family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38289.98 Da        Isoelectric Point: 6.9780

>NTDB_id=756930 OOT43_RS01245 WP_266022834.1 243946..244980(-) (pilT) [Methylococcus mesophilus strain 16-5]
MDIAELLAFSVKNRSSDLHLSSGLPPMIRVDGDIRRINVPPLEHKEVHALVYDIMNDKQRRDFEEFLETDFSFELPGVAR
FRVNAFNQDRGAAAVFRTIPSKVLTLEELGCPKFFQDVTRQPRGLIVVTGPTGSGKSTTLAAMIDYINSNDYSHILTIED
PIEFVHQSKKCLINQREVHRNTLGFNEALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAAEKEMIRSMLSESLQAVISQALIKKAGGGRTAAWEIMVGTPAIRNLIREAKVAQMYSAIQTGRKDGMQTLDQHL
QELVEKGVVTRQVARQIAVNKAAF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=756930 OOT43_RS01245 WP_266022834.1 243946..244980(-) (pilT) [Methylococcus mesophilus strain 16-5]
ATGGATATCGCCGAACTGCTCGCCTTCTCGGTCAAAAACAGATCCTCCGACCTGCATCTATCGTCCGGACTGCCCCCGAT
GATCCGGGTCGACGGCGATATCCGGCGCATCAACGTGCCGCCGCTGGAGCACAAGGAAGTCCACGCCCTGGTCTACGACA
TCATGAACGACAAGCAGCGGCGCGATTTCGAGGAATTCCTGGAAACCGATTTCTCGTTCGAACTGCCCGGCGTCGCGCGT
TTCCGCGTCAACGCGTTCAACCAGGACCGGGGCGCAGCGGCGGTGTTCCGGACCATCCCCTCGAAGGTCCTCACACTAGA
GGAACTGGGCTGCCCGAAATTCTTCCAGGACGTCACCCGCCAGCCGCGCGGATTGATCGTGGTCACCGGTCCCACCGGTT
CGGGCAAGTCCACCACGCTCGCGGCCATGATCGATTACATCAACTCCAACGACTACTCCCATATCCTGACCATCGAAGAT
CCGATCGAATTCGTGCACCAGAGCAAGAAATGCCTGATCAACCAGCGCGAGGTGCACCGCAACACCTTGGGGTTCAACGA
AGCCCTGCGCTCGGCGCTGCGCGAAGACCCGGACGTGATCCTGGTGGGTGAAATGCGCGACCTCGAAACCATCCGTTTGG
CGCTCACCGCGGCCGAAACCGGCCACCTGGTGTTCGGCACCCTGCATACCAGTTCCGCCGCCAAGACCATCGATCGTATC
GTCGACGTATTCCCCGCCGCGGAAAAGGAGATGATCCGCTCCATGCTCTCGGAATCGCTGCAGGCGGTGATCTCGCAGGC
GCTCATCAAGAAAGCCGGCGGCGGCCGGACCGCGGCCTGGGAGATCATGGTCGGCACCCCGGCCATCCGCAACCTGATCC
GGGAGGCCAAGGTCGCCCAGATGTATTCGGCGATCCAAACCGGCCGCAAGGACGGCATGCAGACCCTGGACCAGCACCTG
CAGGAACTGGTGGAAAAAGGGGTCGTGACCCGCCAGGTCGCCCGTCAAATCGCCGTCAATAAAGCCGCGTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

81.065

98.256

0.797
  pilT Acinetobacter nosocomialis M2

78.488

100

0.785
  pilT Acinetobacter baumannii D1279779

78.488

100

0.785
  pilT Acinetobacter baumannii strain A118

78.488

100

0.785
  pilT Pseudomonas stutzeri DSM 10701

78.198

100

0.782
  pilT Acinetobacter baylyi ADP1

76.744

100

0.767
  pilT Legionella pneumophila strain Lp02

73.547

100

0.735
  pilT Legionella pneumophila strain ERS1305867

73.547

100

0.735
  pilT Neisseria meningitidis 8013

67.826

100

0.68
  pilT Neisseria gonorrhoeae MS11

67.246

100

0.674
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.957

100

0.672
  pilT Vibrio cholerae strain A1552

66.957

100

0.672
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

100

0.515
  pilU Pseudomonas stutzeri DSM 10701

40.597

97.384

0.395
  pilU Vibrio cholerae strain A1552

39.067

99.709

0.39
  pilU Acinetobacter baylyi ADP1

39.45

95.058

0.375