Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   OOT43_RS01240 Genome accession   NZ_CP110921
Coordinates   242807..243931 (-) Length   374 a.a.
NCBI ID   WP_266022833.1    Uniprot ID   -
Organism   Methylococcus mesophilus strain 16-5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 237807..248931
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OOT43_RS01215 (OOT43_01215) - 238047..239456 (+) 1410 WP_266022828.1 lyase family protein -
  OOT43_RS01220 (OOT43_01220) - 239489..240271 (+) 783 WP_266022829.1 succinate dehydrogenase iron-sulfur subunit -
  OOT43_RS01225 (OOT43_01225) - 240268..240525 (+) 258 WP_266022830.1 succinate dehydrogenase assembly factor 2 -
  OOT43_RS01230 (OOT43_01230) - 240519..240941 (+) 423 WP_266022831.1 protein YgfX -
  OOT43_RS01235 (OOT43_01235) - 240952..242742 (+) 1791 WP_266022832.1 VWA domain-containing protein -
  OOT43_RS01240 (OOT43_01240) pilU 242807..243931 (-) 1125 WP_266022833.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OOT43_RS01245 (OOT43_01245) pilT 243946..244980 (-) 1035 WP_266022834.1 type IV pilus twitching motility protein PilT Machinery gene
  OOT43_RS01250 (OOT43_01250) - 245034..245723 (+) 690 WP_266022835.1 YggS family pyridoxal phosphate-dependent enzyme -
  OOT43_RS01255 (OOT43_01255) proC 245791..246618 (+) 828 WP_266022836.1 pyrroline-5-carboxylate reductase -
  OOT43_RS01260 (OOT43_01260) - 246618..247196 (+) 579 WP_266022837.1 YggT family protein -

Sequence

Protein


Download         Length: 374 a.a.        Molecular weight: 41965.29 Da        Isoelectric Point: 5.8673

>NTDB_id=756929 OOT43_RS01240 WP_266022833.1 242807..243931(-) (pilU) [Methylococcus mesophilus strain 16-5]
MEFESLLKLMVLKKASDLFITAAKEPCMKVDGAIVPLSSTKLSAEQVRQLVLGIMNQRQRDEFENTNECNFALSATGLGR
FRVSAFIQRNSPGMVLRRIETEIPTVEQLNLPPILNDLVMTKRGLILFVGATGTGKSTSLAAMLKYRNEHSSGHIITIED
PLEFVHPHAGCIVTQREVGIDTESYEVALKNTLRQAPDVILIGEIRTRETMQQAITFAETGHLCLSTLHANNANQALDRI
LHFFPEDMHPQLFMDLSLNLRAIVAQQLVKRADGKGRYPAVEILINTPLVSDLIRQGEVHKLKDVMRQSREQGMQTFDQA
LFELFKAGRIGYEDALYSADSKNEVRLMIKLSEEGSIDKYAPKDDSIRIVEDDE

Nucleotide


Download         Length: 1125 bp        

>NTDB_id=756929 OOT43_RS01240 WP_266022833.1 242807..243931(-) (pilU) [Methylococcus mesophilus strain 16-5]
ATGGAATTCGAATCGCTGCTCAAGCTGATGGTACTGAAGAAAGCGTCCGATCTCTTCATCACCGCGGCCAAGGAGCCCTG
CATGAAGGTCGACGGCGCCATCGTGCCGCTGTCCAGCACCAAGCTGAGCGCCGAACAGGTGCGTCAACTCGTACTCGGCA
TCATGAACCAGCGCCAGCGCGACGAGTTCGAAAACACCAACGAATGCAACTTCGCACTGTCCGCGACCGGGCTTGGCAGG
TTCCGGGTCAGCGCCTTCATCCAGCGCAACAGTCCCGGCATGGTGCTGCGCCGGATCGAAACCGAGATTCCCACCGTGGA
ACAGCTCAACCTGCCACCCATCCTCAACGACCTGGTCATGACCAAGCGCGGCCTGATCCTGTTCGTGGGCGCGACCGGCA
CCGGCAAGTCCACTTCGCTGGCGGCGATGCTCAAGTACCGCAACGAGCACTCCAGCGGCCACATCATCACCATCGAGGAT
CCCCTGGAGTTCGTACACCCGCACGCGGGCTGCATCGTCACCCAGCGCGAAGTCGGCATCGACACCGAATCCTACGAAGT
CGCGCTGAAGAACACCCTGCGGCAGGCGCCGGACGTAATCCTCATCGGCGAGATCCGCACCCGCGAGACCATGCAGCAGG
CCATCACCTTCGCCGAAACCGGCCACCTCTGCCTGAGCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
CTCCATTTCTTCCCGGAAGACATGCATCCGCAGCTGTTCATGGACCTGTCGCTGAACCTGCGCGCCATCGTCGCCCAGCA
GCTCGTCAAGCGCGCCGACGGCAAGGGGCGCTATCCAGCGGTGGAAATCCTCATCAATACCCCTCTGGTCTCGGACCTGA
TCCGCCAGGGCGAGGTCCACAAGCTCAAGGACGTGATGAGACAGTCGCGCGAACAGGGCATGCAGACCTTCGACCAGGCG
CTGTTCGAATTGTTCAAGGCGGGCAGGATCGGCTACGAGGATGCCCTCTACTCCGCCGATTCCAAAAACGAAGTCCGCTT
GATGATCAAGCTCAGCGAAGAAGGCAGCATCGACAAATACGCGCCCAAGGACGACTCAATCCGGATCGTCGAGGACGACG
AGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

69.492

94.652

0.658
  pilU Acinetobacter baylyi ADP1

62.903

99.465

0.626
  pilU Vibrio cholerae strain A1552

58

93.583

0.543
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.391

93.048

0.404
  pilT Legionella pneumophila strain Lp02

41.399

91.711

0.38
  pilT Legionella pneumophila strain ERS1305867

41.399

91.711

0.38
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.617

89.305

0.372
  pilT Vibrio cholerae strain A1552

41.617

89.305

0.372
  pilT Acinetobacter baylyi ADP1

40.469

91.176

0.369
  pilT Acinetobacter nosocomialis M2

40.118

90.642

0.364
  pilT Acinetobacter baumannii D1279779

40.118

90.642

0.364
  pilT Acinetobacter baumannii strain A118

40.118

90.642

0.364
  pilT Pseudomonas aeruginosa PAK

40.597

89.572

0.364