Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   BV903_RS20280 Genome accession   NZ_CP110813
Coordinates   4920707..4921744 (-) Length   345 a.a.
NCBI ID   WP_057946565.1    Uniprot ID   A0A0S2DD87
Organism   Lysobacter enzymogenes strain B25     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4915707..4926744
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BV903_RS20275 (BV903_020275) pilU 4919428..4920594 (-) 1167 WP_057946566.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  BV903_RS20280 (BV903_020280) pilT 4920707..4921744 (-) 1038 WP_057946565.1 type IV pilus twitching motility protein PilT Machinery gene
  BV903_RS20285 (BV903_020285) - 4921924..4922616 (+) 693 WP_267271955.1 YggS family pyridoxal phosphate-dependent enzyme -
  BV903_RS20290 (BV903_020290) - 4922647..4923276 (-) 630 WP_078997834.1 HAD family hydrolase -
  BV903_RS20295 (BV903_020295) proC 4923388..4924224 (+) 837 WP_078997833.1 pyrroline-5-carboxylate reductase -
  BV903_RS20300 (BV903_020300) - 4924221..4924682 (+) 462 WP_078997832.1 DUF4426 domain-containing protein -
  BV903_RS20305 (BV903_020305) - 4925037..4926059 (-) 1023 WP_101943889.1 lipase secretion chaperone -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38500.34 Da        Isoelectric Point: 6.7271

>NTDB_id=756417 BV903_RS20280 WP_057946565.1 4920707..4921744(-) (pilT) [Lysobacter enzymogenes strain B25]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDFEEFLEVDFSFEIPGLAR
FRVNAFNQNRGAGAVFRTIPSEVLSLEDLGCPPIFRQLIEQPQGLILVTGPTGSGKSTTLAAMIDHINKNEYAHILSVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDYILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTVDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGIPAIRNLIREDKVAQMYSAIQTGQAYGMMTLDQHL
QDLVKRGLILRPQAKEYAKDKRLFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=756417 BV903_RS20280 WP_057946565.1 4920707..4921744(-) (pilT) [Lysobacter enzymogenes strain B25]
ATGGATATCGCCGAACTTCTGGCGTTTTCGGTAAAGAACAAAGCATCCGACTTGCATCTTTCGGCCGGCCTGCCGCCGAT
GATCCGGGTCGATGGCGACGTGCGCCGGATCAACATCCCGGCCTTGGACCACAAGCAGGTCCACGCCCTGGTGTACGACA
TCATGTCGGACAAGCAGCGGCGCGATTTCGAAGAGTTCCTGGAAGTCGACTTCTCGTTCGAGATCCCCGGGCTGGCGCGC
TTCCGCGTCAACGCCTTCAACCAGAACCGCGGCGCCGGCGCGGTGTTCCGCACCATTCCCTCCGAGGTGCTGAGCCTGGA
GGACCTGGGCTGCCCGCCGATCTTCCGCCAGCTGATCGAGCAGCCGCAGGGCCTGATCCTGGTGACCGGGCCGACCGGCT
CGGGCAAGTCGACCACGCTCGCGGCGATGATCGACCACATCAACAAGAACGAGTACGCGCACATCCTCTCGGTCGAAGAC
CCGATCGAGTTCGTGCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAAGTCCATCGCGACACGCACGGCTTCAACGA
GGCGCTGCGTTCGGCGCTGCGCGAAGACCCCGACTACATCCTGGTCGGCGAGTTGCGCGATCTGGAAACCATCCGCCTGG
CGCTGACCGCGGCGGAAACCGGCCACCTCGTGTTCGGCACCCTGCACACCTCCTCGGCGGCCAAGACCGTGGACCGCATC
ATCGACGTGTTCCCGGCCGGCGAGAAGCCGATGGTGCGTTCGATGCTGTCCGAATCGCTGCGCGCGGTGATCTCGCAGGC
GCTGCTGAAGAAGGTCGGCGGCGGCCGCACCGCGGCCTGGGAGATCATGGTCGGCATCCCGGCGATCCGTAACCTCATCC
GCGAGGACAAGGTCGCGCAGATGTACTCGGCGATCCAGACCGGCCAGGCCTACGGCATGATGACCCTGGACCAGCACCTC
CAGGACCTGGTCAAGCGCGGCCTGATCCTGCGCCCGCAGGCCAAGGAATACGCCAAGGACAAGCGCCTGTTCGAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0S2DD87

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

78.779

99.71

0.786
  pilT Acinetobacter baumannii D1279779

78.779

99.71

0.786
  pilT Acinetobacter baumannii strain A118

78.779

99.71

0.786
  pilT Pseudomonas stutzeri DSM 10701

78.198

99.71

0.78
  pilT Acinetobacter baylyi ADP1

77.326

99.71

0.771
  pilT Pseudomonas aeruginosa PAK

77.876

98.261

0.765
  pilT Legionella pneumophila strain Lp02

74.128

99.71

0.739
  pilT Legionella pneumophila strain ERS1305867

74.128

99.71

0.739
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.909

95.362

0.667
  pilT Vibrio cholerae strain A1552

69.909

95.362

0.667
  pilT Neisseria meningitidis 8013

66.569

98.841

0.658
  pilT Neisseria gonorrhoeae MS11

66.276

98.841

0.655
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.275

100

0.493
  pilU Acinetobacter baylyi ADP1

40

98.551

0.394
  pilU Pseudomonas stutzeri DSM 10701

40.909

95.652

0.391
  pilU Vibrio cholerae strain A1552

40.308

94.203

0.38