Detailed information NTG0756416

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: BV903_RS20275
• Genome accession: NZ_CP110813
• Coordinates: 4919428..4920594 (-)
• Length: 388 a.a.
• NCBI ID: WP_057946566.1
• Uniprot ID: A0A0S2DDC7
• Organism: Lysobacter enzymogenes strain B25
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4914428..4925594

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BV903_RS20270 (BV903_020270)	-	4915175..4919206 (-)	4032	WP_267271954.1	DUF6531 domain-containing protein	-
BV903_RS20275 (BV903_020275)	pilU	4919428..4920594 (-)	1167	WP_057946566.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
BV903_RS20280 (BV903_020280)	pilT	4920707..4921744 (-)	1038	WP_057946565.1	type IV pilus twitching motility protein PilT	Machinery gene
BV903_RS20285 (BV903_020285)	-	4921924..4922616 (+)	693	WP_267271955.1	YggS family pyridoxal phosphate-dependent enzyme	-
BV903_RS20290 (BV903_020290)	-	4922647..4923276 (-)	630	WP_078997834.1	HAD family hydrolase	-
BV903_RS20295 (BV903_020295)	proC	4923388..4924224 (+)	837	WP_078997833.1	pyrroline-5-carboxylate reductase	-
BV903_RS20300 (BV903_020300)	-	4924221..4924682 (+)	462	WP_078997832.1	DUF4426 domain-containing protein	-

Sequence

Protein

• Length: 388 a.a.
• Molecular weight: 43065.41 Da
• Isoelectric Point: 6.8286

>NTDB_id=756416 BV903_RS20275 WP_057946566.1 4919428..4920594(-) (pilU) [Lysobacter enzymogenes strain B25]
MNTPAPSNPTTASGAIDFTSFLKLMAHQRASDLFITAGMPPSMKVHGKITPITQNPLTPQQSRDLVLNVMSPQQREEFEK
THECNFAIGVTGVGRFRVSCFYQRNQVGMVLRRIETKIPSVDELNLPPIIKTLAMTKRGIIIFVGATGTGKSTSLAAMIG
YRNQNSTGHIITIEDPIEFVHRHEGCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVL
CTLHANNANQAMDRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGKGRRVAMEILLGTPLAQDYIRDGEIHKLKEL
MKESTQLGMKTFDQALFELYQGGEISYEDALRYADSQNEVRLRIKLAQGGDARTLSQGLDGVEVAEVR

Nucleotide

• Length: 1167 bp

>NTDB_id=756416 BV903_RS20275 WP_057946566.1 4919428..4920594(-) (pilU) [Lysobacter enzymogenes strain B25]
ATGAACACGCCCGCCCCGTCCAACCCGACCACCGCCTCCGGCGCGATCGACTTCACCTCCTTCCTCAAGCTGATGGCGCA
CCAGCGCGCCTCGGACTTGTTCATCACCGCCGGCATGCCGCCGTCGATGAAGGTGCACGGCAAGATCACCCCGATCACCC
AGAACCCGCTGACCCCGCAGCAGAGCCGCGACCTCGTCCTCAACGTGATGAGCCCGCAGCAGCGCGAGGAGTTCGAAAAG
ACCCACGAGTGCAACTTCGCCATCGGCGTCACCGGCGTCGGCCGCTTCCGCGTGAGCTGCTTCTACCAGCGCAACCAGGT
CGGCATGGTGCTGCGCCGGATCGAGACCAAGATCCCCAGCGTCGACGAGCTGAACCTGCCGCCGATCATCAAGACGCTGG
CGATGACCAAGCGCGGCATCATCATCTTCGTCGGCGCCACCGGCACCGGCAAGTCGACCTCGCTGGCGGCGATGATCGGC
TACCGCAACCAGAACTCGACCGGCCACATCATCACCATCGAGGACCCGATCGAGTTCGTGCACCGCCACGAGGGCTGCAT
CATCACCCAGCGCGAAGTCGGCATCGACACCGACAGCTGGGAAGCCGCGCTCAAGAACACCCTGCGCCAGGCGCCGGACG
TGATCATGATCGGCGAGGTGCGCACCCGCGAAGGCATGGACCACGCCATCGCCTTCGCCGAAACCGGCCACCTGGTGCTG
TGCACCCTGCACGCCAACAACGCCAACCAGGCGATGGACCGCATCATCAACTTCTTCCCCGAAGATCGCCGCAACCAGCT
GTTGATGGACTTGTCGCTGAACCTCAAGGGCGTGGTCGCCCAGCAGCTGATCCCGACCCCCGACGGCAAGGGCCGGCGCG
TGGCGATGGAGATCCTGCTCGGCACCCCGCTGGCGCAGGACTACATCCGCGACGGCGAGATCCACAAGCTCAAGGAGCTG
ATGAAGGAATCCACCCAGTTGGGCATGAAGACCTTCGACCAGGCCCTGTTCGAGCTCTACCAGGGCGGCGAGATCAGCTA
CGAGGACGCGCTGCGCTACGCCGACTCGCAGAACGAAGTGCGCCTGCGGATCAAGCTGGCGCAGGGCGGCGACGCGCGGA
CCTTGTCGCAGGGGTTGGATGGGGTGGAGGTGGCGGAGGTGCGGTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (130-284)

3D structure

• AlphaFold DB: A0A0S2DDC7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	69.21	94.588	0.655
pilU	Acinetobacter baylyi ADP1	65.546	92.01	0.603
pilU	Vibrio cholerae strain A1552	53.09	91.753	0.487
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.595	90.464	0.376