Detailed information NTG0075449

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: VEGS_RS03720
• Genome accession: NZ_AP021891
• Coordinates: 770211..771191 (+)
• Length: 326 a.a.
• NCBI ID: WP_001618842.1
• Uniprot ID: A0AAF0HMM4
• Organism: Escherichia coli strain 2017.01.04CC
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 765211..776191

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
VEGS_RS03690 (VEGS01_07220)	yggM	765726..766733 (+)	1008	WP_001460056.1	DUF1202 family protein	-
VEGS_RS03695 (VEGS01_07230)	hemW	766888..768024 (-)	1137	WP_000239928.1	radical SAM family heme chaperone HemW	-
VEGS_RS03700 (VEGS01_07240)	rdgB	768017..768610 (-)	594	WP_001174735.1	XTP/dITP diphosphatase	-
VEGS_RS03705 (VEGS01_07250)	yggU	768618..768908 (-)	291	WP_000994920.1	DUF167 family protein YggU	-
VEGS_RS03710 (VEGS01_07260)	yggT	768905..769471 (-)	567	WP_001094831.1	osmotic shock tolerance protein YggT	-
VEGS_RS03715 (VEGS01_07270)	yggS	769489..770193 (-)	705	WP_000997795.1	pyridoxal phosphate homeostasis protein	-
VEGS_RS03720 (VEGS01_07280)	pilT	770211..771191 (+)	981	WP_001618842.1	type IV pilus twitching motility protein PilT	Machinery gene
VEGS_RS03725 (VEGS01_07290)	ruvX	771375..771791 (-)	417	WP_000017106.1	Holliday junction resolvase RuvX	-
VEGS_RS03730 (VEGS01_07300)	yqgE	771791..772354 (-)	564	WP_001053178.1	YqgE/AlgH family protein	-
VEGS_RS03735 (VEGS01_07310)	gshB	772463..773413 (-)	951	WP_000593273.1	glutathione synthase	-
VEGS_RS03740 (VEGS01_07320)	rsmE	773426..774157 (-)	732	WP_001300912.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
VEGS_RS03745 (VEGS01_07330)	endA	774237..774944 (-)	708	WP_000286500.1	deoxyribonuclease I	-
VEGS_RS03750 (VEGS01_07340)	yggI	775039..775536 (-)	498	WP_001300769.1	SprT family zinc-dependent metalloprotease	-

Sequence

Protein

• Length: 326 a.a.
• Molecular weight: 35937.10 Da
• Isoelectric Point: 5.9172

>NTDB_id=75449 VEGS_RS03720 WP_001618842.1 770211..771191(+) (pilT) [Escherichia coli strain 2017.01.04CC]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRHGISLALRLLPSHCPQLEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQSVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQHR
VGEGRL

Nucleotide

• Length: 981 bp

>NTDB_id=75449 VEGS_RS03720 WP_001618842.1 770211..771191(+) (pilT) [Escherichia coli strain 2017.01.04CC]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCTAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGATTGCGTGGC
AGCGCATTCGCGCAACGGCATGGCATTTCGCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCAACGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCACTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCCGCGCAGTCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCAGGGAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTATTTGAATTGCTGATTAACACACCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCACCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (62-239)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	49.235	100	0.494
pilT	Vibrio cholerae strain A1552	49.235	100	0.494
pilT	Neisseria meningitidis 8013	48.78	100	0.491
pilT	Neisseria gonorrhoeae MS11	48.476	100	0.488
pilT	Acinetobacter baumannii strain A118	46.483	100	0.466
pilT	Acinetobacter baumannii D1279779	46.483	100	0.466
pilT	Acinetobacter nosocomialis M2	46.483	100	0.466
pilT	Pseudomonas stutzeri DSM 10701	46.483	100	0.466
pilT	Acinetobacter baylyi ADP1	46.177	100	0.463
pilT	Pseudomonas aeruginosa PAK	45.872	100	0.46
pilT	Legionella pneumophila strain ERS1305867	45.912	97.546	0.448
pilT	Legionella pneumophila strain Lp02	45.912	97.546	0.448
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.453	97.546	0.414
pilU	Vibrio cholerae strain A1552	40.062	98.773	0.396
pilU	Pseudomonas stutzeri DSM 10701	37.273	100	0.377
pilU	Acinetobacter baylyi ADP1	36.747	100	0.374
pilB	Legionella pneumophila strain ERS1305867	30.89	100	0.362