Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HRR27_RS02930 Genome accession   NZ_AP021889
Coordinates   649587..650624 (-) Length   345 a.a.
NCBI ID   WP_173270688.1    Uniprot ID   A0A6F8PSV5
Organism   Thiosulfatimonas sediminis strain aks77     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 644587..655624
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HRR27_RS02910 (THMIRHAS_05820) - 645460..646389 (+) 930 WP_173270679.1 hypothetical protein -
  HRR27_RS02915 (THMIRHAS_05830) - 646376..647443 (+) 1068 WP_173270682.1 L,D-transpeptidase family protein -
  HRR27_RS02920 (THMIRHAS_05840) - 647494..648429 (-) 936 WP_243830869.1 M14 family zinc carboxypeptidase -
  HRR27_RS02925 (THMIRHAS_05850) pilU 648426..649577 (-) 1152 WP_173270685.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HRR27_RS02930 (THMIRHAS_05860) pilT 649587..650624 (-) 1038 WP_173270688.1 type IV pilus twitching motility protein PilT Machinery gene
  HRR27_RS02935 (THMIRHAS_05870) - 650826..651284 (-) 459 WP_173270691.1 DNA polymerase III subunit chi -
  HRR27_RS02940 (THMIRHAS_05880) - 651281..652768 (-) 1488 WP_341829818.1 leucyl aminopeptidase -
  HRR27_RS02945 (THMIRHAS_05890) lptF 652968..654107 (+) 1140 WP_173270694.1 LPS export ABC transporter permease LptF -
  HRR27_RS02950 (THMIRHAS_05900) - 654104..655249 (+) 1146 WP_173270699.1 LptF/LptG family permease -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38288.85 Da        Isoelectric Point: 6.1063

>NTDB_id=75413 HRR27_RS02930 WP_173270688.1 649587..650624(-) (pilT) [Thiosulfatimonas sediminis strain aks77]
MDITSLLSFSVEQGASDLHLSAGQPPILRINGDITRIDAADFEEEQLQLMIYDVMNDAQRRHFEAELESDFSFEIPNLAR
FRVNVFRQNRGIGAVFRTIPSTILTLEQLKAPAIFKDISSFPRGIVLVTGPTGSGKSTTLAAMIDYVNKNFASHILTIED
PIEFVHTPILSLINQREVHRDTKSFSNSLRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKDMVRSMLSESLRAVISQTLLKSTAGGRVAAHEIMLATTAIRNLIREAKIPQMYSVIQTSNQLGMLTLDQNL
KQLVDNGIITREAARSKAVNKQNFT

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=75413 HRR27_RS02930 WP_173270688.1 649587..650624(-) (pilT) [Thiosulfatimonas sediminis strain aks77]
ATGGACATTACCAGCCTACTGTCATTCAGTGTTGAACAAGGCGCATCCGACCTCCATCTCTCTGCAGGTCAACCGCCGAT
TTTACGGATCAACGGCGACATTACTCGTATCGATGCTGCAGACTTTGAAGAAGAACAGCTTCAATTGATGATTTATGACG
TGATGAACGATGCTCAACGTCGTCATTTTGAAGCCGAACTGGAATCCGACTTCTCCTTTGAAATCCCTAATTTAGCGCGT
TTCAGGGTGAATGTATTTCGTCAGAACCGAGGTATTGGCGCGGTATTCCGTACCATCCCCAGCACCATTCTCACCTTAGA
GCAATTAAAAGCGCCAGCAATCTTTAAAGACATCTCCAGCTTTCCGCGTGGCATCGTACTGGTGACCGGCCCGACGGGAT
CGGGGAAATCAACAACCCTGGCTGCAATGATTGATTATGTGAACAAAAATTTTGCCTCACACATTTTGACCATCGAAGAC
CCGATCGAATTTGTTCATACCCCAATTTTGAGTTTGATTAACCAACGTGAAGTGCATCGTGACACCAAAAGCTTTAGCAA
CTCCTTGCGTTCAGCACTGCGTGAAGACCCAGACGTTATCCTTGTTGGGGAGATGCGTGATTTAGAAACCATTCGCCTTG
CACTGACCGCCGCAGAAACCGGACACTTAGTGTTCGGTACTCTGCACACCAGCTCGGCTGCGAAAACCATCGACCGGATT
ATTGATGTCTTTCCAGCCGAAGAAAAAGACATGGTGCGCTCAATGCTATCCGAATCGCTGCGAGCGGTTATTTCACAAAC
TCTACTAAAAAGTACTGCCGGTGGACGAGTTGCCGCACATGAAATTATGCTTGCGACCACGGCAATCCGTAACCTTATTC
GTGAAGCGAAAATCCCACAAATGTATTCGGTCATTCAGACCTCGAATCAACTGGGTATGCTGACACTTGACCAAAACTTA
AAACAGTTGGTTGATAACGGCATTATTACCCGTGAAGCGGCACGCTCGAAAGCCGTTAATAAACAGAACTTTACTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6F8PSV5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

70.93

99.71

0.707
  pilT Pseudomonas aeruginosa PAK

70.64

99.71

0.704
  pilT Acinetobacter baumannii strain A118

70.349

99.71

0.701
  pilT Acinetobacter nosocomialis M2

70.349

99.71

0.701
  pilT Acinetobacter baumannii D1279779

70.349

99.71

0.701
  pilT Pseudomonas stutzeri DSM 10701

69.477

99.71

0.693
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.953

97.681

0.664
  pilT Vibrio cholerae strain A1552

67.953

97.681

0.664
  pilT Legionella pneumophila strain Lp02

66.279

99.71

0.661
  pilT Legionella pneumophila strain ERS1305867

66.279

99.71

0.661
  pilT Neisseria gonorrhoeae MS11

64.74

100

0.649
  pilT Neisseria meningitidis 8013

64.74

100

0.649
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.437

100

0.519
  pilU Pseudomonas stutzeri DSM 10701

42.388

97.101

0.412
  pilU Acinetobacter baylyi ADP1

39.595

100

0.397
  pilU Vibrio cholerae strain A1552

38.757

97.971

0.38

Multiple sequence alignment