Detailed information NTG0075412

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: HRR27_RS02925
• Genome accession: NZ_AP021889
• Coordinates: 648426..649577 (-)
• Length: 383 a.a.
• NCBI ID: WP_173270685.1
• Uniprot ID: -
• Organism: Thiosulfatimonas sediminis strain aks77
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 643426..654577

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HRR27_RS02910 (THMIRHAS_05820)	-	645460..646389 (+)	930	WP_173270679.1	hypothetical protein	-
HRR27_RS02915 (THMIRHAS_05830)	-	646376..647443 (+)	1068	WP_173270682.1	L,D-transpeptidase family protein	-
HRR27_RS02920 (THMIRHAS_05840)	-	647494..648429 (-)	936	WP_243830869.1	M14 family zinc carboxypeptidase	-
HRR27_RS02925 (THMIRHAS_05850)	pilU	648426..649577 (-)	1152	WP_173270685.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
HRR27_RS02930 (THMIRHAS_05860)	pilT	649587..650624 (-)	1038	WP_173270688.1	type IV pilus twitching motility protein PilT	Machinery gene
HRR27_RS02935 (THMIRHAS_05870)	-	650826..651284 (-)	459	WP_173270691.1	DNA polymerase III subunit chi	-
HRR27_RS02940 (THMIRHAS_05880)	-	651281..652768 (-)	1488	WP_341829818.1	leucyl aminopeptidase	-
HRR27_RS02945 (THMIRHAS_05890)	lptF	652968..654107 (+)	1140	WP_173270694.1	LPS export ABC transporter permease LptF	-

Sequence

Protein

• Length: 383 a.a.
• Molecular weight: 42659.74 Da
• Isoelectric Point: 6.2351

>NTDB_id=75412 HRR27_RS02925 WP_173270685.1 648426..649577(-) (pilU) [Thiosulfatimonas sediminis strain aks77]
MEQEFSDLQKLLIHFSENNGSDLFITAGRPIAMKQNGRLVDLTENKVTPDMSRELCYEIMNHDQLSTFQATSEANFAYRI
TGRARYRVNVFVQRGSPGMVIRSVQTEIPSFEALNLPPILREQVLKKRGLILIVGGTGSGKSTTLASMIGVRNHETEGHI
ITIEDPIEFVHRHAGCIVTQREVGVDTDSYENALKNTLRQAPDVILIGEIRARETMEHAIAFAETGHLCLSTLHANNTNQ
ALDRIINFFPIDHKNHLLLDLSLNLRAIISQRLIPKIGGGMIAAVEVLINSPRVAELVLQGNVSEIKEVMAKSTAQGMQT
FDQHLFQLYESQQISYEDALRNSDAMNDLRLNIKLNSKLPLPEALGGPLAGGNFTLREQSKEE

Nucleotide

• Length: 1152 bp

>NTDB_id=75412 HRR27_RS02925 WP_173270685.1 648426..649577(-) (pilU) [Thiosulfatimonas sediminis strain aks77]
ATGGAACAAGAATTTAGCGACCTACAAAAACTCCTGATTCACTTCTCAGAAAACAACGGTTCCGATCTATTCATTACCGC
TGGGCGACCGATTGCTATGAAACAAAACGGCCGTTTGGTGGATTTAACCGAAAACAAAGTCACCCCAGATATGTCGCGTG
AACTCTGTTATGAAATCATGAACCACGACCAGCTGTCCACTTTCCAAGCTACCTCGGAAGCAAACTTTGCATACCGCATC
ACCGGCCGCGCCCGATATCGTGTTAATGTATTTGTGCAACGCGGCTCGCCAGGGATGGTTATTCGTTCAGTACAAACCGA
AATCCCCAGTTTTGAGGCCCTTAATTTGCCGCCGATACTTAGGGAACAAGTGCTCAAAAAACGTGGGTTGATTCTGATTG
TTGGTGGAACCGGTTCCGGTAAATCAACCACTTTAGCAAGTATGATTGGAGTACGTAATCACGAAACCGAAGGCCATATT
ATTACCATCGAAGACCCGATCGAATTTGTCCATCGTCACGCTGGCTGCATCGTCACTCAACGCGAGGTGGGAGTGGATAC
CGACTCTTACGAAAACGCCCTAAAAAACACTTTACGACAAGCGCCAGACGTCATTCTTATCGGTGAGATTCGTGCCAGAG
AGACCATGGAGCACGCCATCGCTTTCGCAGAAACTGGTCACTTATGCCTCTCCACACTGCACGCTAACAACACTAACCAG
GCCCTTGACCGAATTATTAACTTTTTTCCAATCGACCATAAAAACCATCTTTTACTCGACCTTTCACTTAACTTACGTGC
TATTATTTCGCAGCGTCTTATCCCTAAAATTGGTGGTGGAATGATTGCTGCGGTGGAAGTTTTAATTAATTCTCCGCGCG
TAGCCGAGCTGGTTTTACAAGGAAATGTCAGTGAAATCAAAGAAGTTATGGCTAAGTCCACCGCGCAAGGGATGCAGACA
TTTGACCAGCATCTATTCCAATTGTATGAGTCACAACAGATTAGCTACGAAGACGCACTGCGCAACTCCGATGCGATGAA
CGACTTACGTCTAAACATAAAATTAAACAGTAAATTACCTCTGCCAGAAGCGCTCGGCGGCCCCCTAGCAGGGGGGAACT
TTACCCTTAGAGAACAGAGCAAGGAAGAATGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (20-276)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	58.146	92.95	0.54
pilU	Acinetobacter baylyi ADP1	55.923	94.778	0.53
pilU	Vibrio cholerae strain A1552	54.142	88.251	0.478