Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   THMIRH_RS02660 Genome accession   NZ_AP021888
Coordinates   634605..635642 (-) Length   345 a.a.
NCBI ID   WP_173290497.1    Uniprot ID   A0A6F8PL14
Organism   Thiosulfativibrio zosterae strain AkT22     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 629605..640642
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  THMIRH_RS02640 (THMIRHAT_05180) - 630476..631336 (+) 861 WP_173290491.1 tetratricopeptide repeat protein -
  THMIRH_RS02645 (THMIRHAT_05190) - 631333..632436 (+) 1104 WP_173290493.1 L,D-transpeptidase family protein -
  THMIRH_RS02650 (THMIRHAT_05200) - 632511..633440 (-) 930 WP_243831478.1 M14 family zinc carboxypeptidase -
  THMIRH_RS02655 (THMIRHAT_05210) pilU 633463..634596 (-) 1134 WP_173290495.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  THMIRH_RS02660 (THMIRHAT_05220) pilT 634605..635642 (-) 1038 WP_173290497.1 type IV pilus twitching motility protein PilT Machinery gene
  THMIRH_RS02665 (THMIRHAT_05230) - 635652..636122 (-) 471 WP_173290499.1 DNA polymerase III subunit chi -
  THMIRH_RS02670 (THMIRHAT_05240) - 636123..637613 (-) 1491 WP_173290501.1 leucyl aminopeptidase -
  THMIRH_RS02675 (THMIRHAT_05250) lptF 637683..638795 (+) 1113 WP_173290503.1 LPS export ABC transporter permease LptF -
  THMIRH_RS02680 (THMIRHAT_05260) lptG 638792..639913 (+) 1122 WP_173290505.1 LPS export ABC transporter permease LptG -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38413.20 Da        Isoelectric Point: 6.8930

>NTDB_id=75401 THMIRH_RS02660 WP_173290497.1 634605..635642(-) (pilT) [Thiosulfativibrio zosterae strain AkT22]
MDITQLLQFAQQQGASDLHLSAGLPPMLRSDGDIRKVNLPDLDPEEIHRMIYDVMNDQQRKNFEEHLEADFSFELPKVAR
FRVNVFQQNRGIAAVFRTIPSKVLSLADLKAPELFKEIAMTPRGIVLVTGPTGSGKSTTLAAMVDHINQTQYSHILTIED
PIEFVHQSQRALINQREVHRDTHSFSNALRSSLREDPDVILVGEMRDLETIRLALTAAETGHLVLGTLHTSSAPKTIDRI
IDVFPAAEKDMVRAMLSESLKAVISQTLMKKVGGGRVAAHEIMLGTPAICNLIREDKIPQMRSTIETSNKAGMQSLDQDL
KRLVAQGLVTKDDARKKAVVKQDFV

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=75401 THMIRH_RS02660 WP_173290497.1 634605..635642(-) (pilT) [Thiosulfativibrio zosterae strain AkT22]
ATGGACATTACCCAGCTACTACAATTTGCTCAACAACAAGGTGCTTCAGACTTGCACTTATCTGCTGGGCTGCCCCCCAT
GTTGCGTAGCGATGGCGATATTCGTAAAGTGAACCTACCAGACTTAGACCCAGAAGAAATTCATCGCATGATTTATGATG
TGATGAATGACCAACAACGCAAAAACTTTGAAGAACACCTAGAAGCCGACTTTTCTTTTGAACTGCCTAAAGTTGCGCGC
TTTAGGGTGAATGTGTTTCAACAAAACCGCGGCATAGCCGCTGTTTTTAGAACCATTCCCAGTAAAGTGTTATCGCTTGC
CGATCTCAAAGCACCAGAGCTTTTCAAAGAAATTGCCATGACGCCGCGCGGCATTGTTTTGGTCACAGGACCTACCGGCT
CAGGAAAATCCACCACCCTAGCGGCAATGGTAGACCACATCAACCAAACGCAATACAGTCATATACTCACCATCGAAGAC
CCTATTGAGTTTGTGCATCAATCCCAAAGAGCGCTGATTAATCAGCGTGAAGTGCATCGTGACACCCACAGCTTCAGTAA
TGCTCTACGCTCCTCGTTACGCGAAGACCCTGATGTTATTTTGGTGGGTGAGATGCGTGATCTTGAAACCATCCGCTTGG
CTTTAACGGCGGCGGAAACCGGGCATTTGGTTCTGGGGACTTTACACACCAGTTCTGCGCCCAAAACCATTGACCGTATT
ATTGATGTCTTTCCTGCCGCCGAAAAAGATATGGTGCGCGCCATGTTGTCAGAATCCTTAAAAGCAGTTATTTCTCAAAC
CCTCATGAAAAAAGTCGGGGGTGGTCGTGTAGCCGCTCACGAAATCATGTTGGGAACACCGGCCATTTGCAATTTGATTA
GAGAAGATAAAATTCCACAGATGCGCTCGACCATAGAAACCTCTAACAAGGCAGGTATGCAGAGTTTAGACCAAGACTTA
AAACGATTGGTTGCTCAAGGCCTGGTCACAAAAGATGATGCCCGCAAAAAAGCGGTCGTTAAACAGGACTTTGTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6F8PL14

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

71.802

99.71

0.716
  pilT Pseudomonas aeruginosa PAK

71.512

99.71

0.713
  pilT Acinetobacter baumannii D1279779

70.725

100

0.707
  pilT Acinetobacter baumannii strain A118

70.725

100

0.707
  pilT Acinetobacter nosocomialis M2

70.435

100

0.704
  pilT Acinetobacter baylyi ADP1

69.186

99.71

0.69
  pilT Legionella pneumophila strain Lp02

65.698

99.71

0.655
  pilT Legionella pneumophila strain ERS1305867

65.698

99.71

0.655
  pilT Neisseria meningitidis 8013

66.372

98.261

0.652
  pilT Neisseria gonorrhoeae MS11

66.077

98.261

0.649
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.469

97.681

0.649
  pilT Vibrio cholerae strain A1552

66.469

97.681

0.649
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.578

100

0.507
  pilU Acinetobacter baylyi ADP1

42.647

98.551

0.42
  pilU Pseudomonas stutzeri DSM 10701

42.687

97.101

0.414
  pilU Vibrio cholerae strain A1552

39.42

100

0.394

Multiple sequence alignment