Detailed information NTG0075400

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: THMIRH_RS02655
• Genome accession: NZ_AP021888
• Coordinates: 633463..634596 (-)
• Length: 377 a.a.
• NCBI ID: WP_173290495.1
• Uniprot ID: A0A6F8PL06
• Organism: Thiosulfativibrio zosterae strain AkT22
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 628463..639596

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
THMIRH_RS02640 (THMIRHAT_05180)	-	630476..631336 (+)	861	WP_173290491.1	tetratricopeptide repeat protein	-
THMIRH_RS02645 (THMIRHAT_05190)	-	631333..632436 (+)	1104	WP_173290493.1	L,D-transpeptidase family protein	-
THMIRH_RS02650 (THMIRHAT_05200)	-	632511..633440 (-)	930	WP_243831478.1	M14 family zinc carboxypeptidase	-
THMIRH_RS02655 (THMIRHAT_05210)	pilU	633463..634596 (-)	1134	WP_173290495.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
THMIRH_RS02660 (THMIRHAT_05220)	pilT	634605..635642 (-)	1038	WP_173290497.1	type IV pilus twitching motility protein PilT	Machinery gene
THMIRH_RS02665 (THMIRHAT_05230)	-	635652..636122 (-)	471	WP_173290499.1	DNA polymerase III subunit chi	-
THMIRH_RS02670 (THMIRHAT_05240)	-	636123..637613 (-)	1491	WP_173290501.1	leucyl aminopeptidase	-
THMIRH_RS02675 (THMIRHAT_05250)	lptF	637683..638795 (+)	1113	WP_173290503.1	LPS export ABC transporter permease LptF	-

Sequence

Protein

• Length: 377 a.a.
• Molecular weight: 41952.26 Da
• Isoelectric Point: 6.3687

>NTDB_id=75400 THMIRH_RS02655 WP_173290495.1 633463..634596(-) (pilU) [Thiosulfativibrio zosterae strain AkT22]
MTPIQNHLHALLTAFVKQKGSDLFITANAPVSYKKDGKMHALTKETLTPEMAEQLTLGLMNESQRQDFLSHQECNFAYQI
PGLSRFRVNAFKQLNQSGLVIRVINSKIPEFADLNLPEVLLDLIMQQRGLILFVGGTGSGKSTSLAALIGHRNANSQGHI
ITIEDPVEFVHPHKGCIVTQREVGVDTESYEIALKNTLRQAPDVILIGEIRAHETMEHAITFAETGHLCLSTLHANNANQ
AIDRIINFFSEERRAQLLMDLSLNLKAIISQRLIPKIGGGRVAAIEILINTPRMADLIFKGQIAEMKTLIKASEEAGMQT
FDQALFKLYEAQQITYEDALRNADSMNDLRLLIKLNSQLPQPQQDEPQAPVTMQTDA

Nucleotide

• Length: 1134 bp

>NTDB_id=75400 THMIRH_RS02655 WP_173290495.1 633463..634596(-) (pilU) [Thiosulfativibrio zosterae strain AkT22]
ATGACGCCTATTCAAAACCACCTCCATGCTCTGCTGACCGCCTTTGTAAAACAAAAAGGCTCTGATTTGTTCATTACCGC
TAATGCGCCGGTTTCTTATAAAAAAGATGGCAAAATGCACGCCCTCACCAAAGAAACCCTCACCCCAGAAATGGCTGAGC
AACTCACGCTGGGTTTAATGAATGAGTCCCAACGCCAAGATTTTTTAAGTCACCAAGAGTGTAACTTTGCTTATCAAATC
CCAGGATTGTCGCGCTTTCGAGTCAATGCTTTTAAGCAATTAAACCAATCAGGTTTGGTCATACGCGTGATTAACAGTAA
AATTCCTGAGTTTGCTGACCTGAATTTACCAGAAGTTCTGCTCGACTTAATCATGCAACAACGCGGCTTAATATTGTTTG
TGGGGGGCACAGGTTCTGGTAAATCAACCTCTCTAGCAGCCCTGATTGGGCATCGTAATGCTAATTCGCAAGGTCATATT
ATTACCATTGAAGACCCGGTAGAGTTTGTGCATCCACACAAAGGTTGTATTGTTACGCAACGAGAAGTCGGTGTAGATAC
TGAGTCTTACGAAATTGCTTTAAAAAACACGCTGCGCCAAGCACCCGATGTGATTTTAATTGGTGAAATCCGGGCTCACG
AAACCATGGAACACGCCATTACCTTTGCTGAAACCGGACATCTGTGCCTGTCAACACTGCATGCAAACAATGCAAACCAA
GCCATAGACCGCATTATTAACTTCTTTTCAGAAGAGCGCCGCGCGCAATTGTTGATGGACTTATCCTTAAACTTAAAAGC
CATTATTTCACAAAGACTGATACCTAAAATTGGTGGCGGTCGAGTGGCCGCCATCGAGATACTGATTAACACGCCACGAA
TGGCCGATTTAATCTTTAAAGGGCAAATTGCAGAAATGAAGACCTTGATTAAGGCCTCAGAAGAAGCCGGTATGCAAACT
TTCGACCAGGCCTTATTTAAACTTTATGAAGCCCAACAAATTACCTATGAAGATGCATTACGCAATGCCGACTCCATGAA
TGATTTACGCTTACTAATTAAGCTAAACAGTCAACTGCCACAGCCGCAGCAAGACGAACCGCAAGCGCCTGTAACCATGC
AAACGGATGCTTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (17-275)

3D structure

• AlphaFold DB: A0A6F8PL06

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	62.464	92.573	0.578
pilU	Acinetobacter baylyi ADP1	57.345	93.899	0.538
pilU	Vibrio cholerae strain A1552	54.31	92.308	0.501
pilT	Pseudomonas aeruginosa PAK	43.544	88.329	0.385
pilT	Pseudomonas stutzeri DSM 10701	42.342	88.329	0.374
pilT	Legionella pneumophila strain ERS1305867	42.553	87.268	0.371
pilT	Legionella pneumophila strain Lp02	42.553	87.268	0.371
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.593	85.942	0.366