Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SFSGTM_RS13395 Genome accession   NZ_AP021881
Coordinates   2722850..2723893 (+) Length   347 a.a.
NCBI ID   WP_162085596.1    Uniprot ID   A0A809RSM7
Organism   Sulfuriferula nivalis strain SGTM     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2717850..2728893
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SFSGTM_RS13375 (SFSGTM_25850) - 2718766..2720688 (-) 1923 WP_162085592.1 dynamin family protein -
  SFSGTM_RS13380 (SFSGTM_25860) - 2720723..2721298 (-) 576 WP_162085593.1 YggT family protein -
  SFSGTM_RS13385 (SFSGTM_25870) proC 2721319..2722119 (-) 801 WP_162085594.1 pyrroline-5-carboxylate reductase -
  SFSGTM_RS13390 (SFSGTM_25880) - 2722122..2722808 (-) 687 WP_162085595.1 YggS family pyridoxal phosphate-dependent enzyme -
  SFSGTM_RS13395 (SFSGTM_25890) pilT 2722850..2723893 (+) 1044 WP_162085596.1 type IV pilus twitching motility protein PilT Machinery gene
  SFSGTM_RS13400 (SFSGTM_25900) pilU 2723897..2725024 (+) 1128 WP_162085597.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SFSGTM_RS13405 (SFSGTM_25910) - 2725072..2726223 (-) 1152 WP_162085598.1 THUMP domain-containing protein -
  SFSGTM_RS13410 (SFSGTM_25920) - 2726220..2726636 (-) 417 WP_162085599.1 CopD family protein -
  SFSGTM_RS13415 (SFSGTM_25930) - 2726649..2727185 (+) 537 WP_162085600.1 TlpA disulfide reductase family protein -
  SFSGTM_RS13420 (SFSGTM_25940) - 2727175..2727714 (-) 540 WP_162085601.1 DinB family protein -
  SFSGTM_RS13425 (SFSGTM_25950) - 2727711..2728214 (-) 504 WP_162085602.1 TlpA disulfide reductase family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38377.05 Da        Isoelectric Point: 6.6647

>NTDB_id=75243 SFSGTM_RS13395 WP_162085596.1 2722850..2723893(+) (pilT) [Sulfuriferula nivalis strain SGTM]
MEISELLAFAVKNKASDLHLSAGLPPMIRVHGDIRRINLPDMNHQTVHDMVYDIMNDGQRKIYEETLEVDFSFEVPTLAR
FRVNAFNQNRGAGAVFRTIPSKVLTLEDLNAPKIFKDIADTPRGIVLVTGPTGSGKSTTLAAMVDYVNENEYGHILTVED
PIEFVHQSKKCVINQREVGPHTLSFSNALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAAEKEMVRSMLSESIRAVISQTLLKTKDGTGRVAAHEIMIGTPAIRNLIRENKVAQMYSSIQTGQNVGMQTLDQC
LQDLVKRNIISIGEARSRAANKDMFLG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=75243 SFSGTM_RS13395 WP_162085596.1 2722850..2723893(+) (pilT) [Sulfuriferula nivalis strain SGTM]
ATGGAAATATCTGAATTACTCGCATTTGCTGTTAAAAACAAAGCATCCGACTTACATTTATCCGCAGGTTTACCGCCTAT
GATACGCGTCCACGGCGATATTAGACGCATTAATCTGCCTGACATGAACCATCAGACCGTACACGACATGGTGTACGACA
TCATGAACGATGGTCAGCGTAAAATCTACGAAGAAACTTTAGAAGTCGATTTTTCATTCGAAGTCCCGACACTAGCGCGT
TTTCGTGTCAACGCTTTTAACCAGAACCGTGGTGCAGGCGCAGTATTCCGTACCATTCCAAGCAAAGTCCTGACACTGGA
GGATCTCAACGCCCCCAAGATTTTCAAAGACATTGCTGATACCCCTCGCGGTATCGTATTGGTCACCGGCCCAACGGGTT
CTGGTAAATCGACCACGCTGGCGGCGATGGTCGATTATGTTAACGAAAATGAATATGGCCATATCCTGACCGTTGAAGAC
CCTATCGAATTTGTCCATCAAAGTAAAAAATGCGTAATCAACCAGCGCGAAGTCGGTCCTCACACACTGTCATTCTCAAA
TGCATTGCGTTCCGCATTGCGTGAAGATCCGGACGTTATTCTCGTCGGCGAGATGCGCGATCTGGAAACCATACGACTCG
CGCTCACCGCTGCTGAAACAGGCCACCTAGTATTTGGTACGCTACACACCAGCTCGGCTGCCAAAACCATAGACCGTATT
GTAGACGTATTTCCAGCGGCAGAAAAAGAGATGGTACGTTCCATGCTATCGGAATCTATCCGCGCCGTTATTTCCCAGAC
CCTGCTCAAAACCAAAGATGGCACAGGTCGAGTTGCCGCTCATGAAATTATGATAGGTACACCTGCCATACGTAACTTGA
TACGTGAAAACAAAGTGGCACAAATGTACTCGTCTATCCAGACCGGTCAGAATGTTGGTATGCAAACGCTGGATCAATGC
CTGCAAGATTTGGTCAAGCGCAATATTATTTCCATCGGCGAAGCACGCTCACGTGCCGCTAATAAAGATATGTTCTTAGG
ATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A809RSM7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

78.613

99.712

0.784
  pilT Acinetobacter baumannii D1279779

78.613

99.712

0.784
  pilT Acinetobacter baumannii strain A118

78.613

99.712

0.784
  pilT Pseudomonas stutzeri DSM 10701

76.232

99.424

0.758
  pilT Acinetobacter baylyi ADP1

76.232

99.424

0.758
  pilT Pseudomonas aeruginosa PAK

75.652

99.424

0.752
  pilT Legionella pneumophila strain Lp02

72.464

99.424

0.72
  pilT Legionella pneumophila strain ERS1305867

72.464

99.424

0.72
  pilT Neisseria meningitidis 8013

72.886

98.847

0.72
  pilT Neisseria gonorrhoeae MS11

72.595

98.847

0.718
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.047

97.406

0.663
  pilT Vibrio cholerae strain A1552

68.047

97.406

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

99.424

0.51
  pilU Pseudomonas stutzeri DSM 10701

42.985

96.542

0.415
  pilU Vibrio cholerae strain A1552

41.994

95.389

0.401
  pilU Acinetobacter baylyi ADP1

40.882

97.983

0.401

Multiple sequence alignment