Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   SFSGTM_RS13400 Genome accession   NZ_AP021881
Coordinates   2723897..2725024 (+) Length   375 a.a.
NCBI ID   WP_162085597.1    Uniprot ID   A0A809SAV2
Organism   Sulfuriferula nivalis strain SGTM     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2718897..2730024
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SFSGTM_RS13380 (SFSGTM_25860) - 2720723..2721298 (-) 576 WP_162085593.1 YggT family protein -
  SFSGTM_RS13385 (SFSGTM_25870) proC 2721319..2722119 (-) 801 WP_162085594.1 pyrroline-5-carboxylate reductase -
  SFSGTM_RS13390 (SFSGTM_25880) - 2722122..2722808 (-) 687 WP_162085595.1 YggS family pyridoxal phosphate-dependent enzyme -
  SFSGTM_RS13395 (SFSGTM_25890) pilT 2722850..2723893 (+) 1044 WP_162085596.1 type IV pilus twitching motility protein PilT Machinery gene
  SFSGTM_RS13400 (SFSGTM_25900) pilU 2723897..2725024 (+) 1128 WP_162085597.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SFSGTM_RS13405 (SFSGTM_25910) - 2725072..2726223 (-) 1152 WP_162085598.1 THUMP domain-containing protein -
  SFSGTM_RS13410 (SFSGTM_25920) - 2726220..2726636 (-) 417 WP_162085599.1 CopD family protein -
  SFSGTM_RS13415 (SFSGTM_25930) - 2726649..2727185 (+) 537 WP_162085600.1 TlpA disulfide reductase family protein -
  SFSGTM_RS13420 (SFSGTM_25940) - 2727175..2727714 (-) 540 WP_162085601.1 DinB family protein -
  SFSGTM_RS13425 (SFSGTM_25950) - 2727711..2728214 (-) 504 WP_162085602.1 TlpA disulfide reductase family protein -
  SFSGTM_RS13430 (SFSGTM_25960) - 2728240..2728944 (+) 705 WP_162085603.1 DUF4197 domain-containing protein -
  SFSGTM_RS13435 (SFSGTM_25970) - 2728944..2729690 (+) 747 WP_162085604.1 type 1 glutamine amidotransferase domain-containing protein -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 41977.32 Da        Isoelectric Point: 6.9829

>NTDB_id=75244 SFSGTM_RS13400 WP_162085597.1 2723897..2725024(+) (pilU) [Sulfuriferula nivalis strain SGTM]
MEALKTVHDLLRLMLSKRASDLFITAGFPPAIKVDGKIAPVSNQTLTPSHTRELARSIMNDRQWHDFEHTHEANFAISPE
GIGRFRVNAFVQQGRIGLVLRTITTLIPKLEQLNLPPVLKDVAMVKRGLVIFVGGTGSGKSTSLAAMVGHRNENAQDHII
TIEDPVEYVHEHKKSIITQREVGIDTDNWFSALKNTLRQAPDVILIGEVRDRETMEYAIAFAETGHLCFTTLHANSANQA
LDRIINFFPDERRQQLLMDLSLNLKAFVSQRLIARKGTTGRIAAVEVLLNSPLISELIFKGEVHEIKAIMAKSREMGMQT
FDQSLFDLYEAGLITYEDALRNADSLNDLRLKIKLEGHEAKGRDVMDGIQSLNIV

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=75244 SFSGTM_RS13400 WP_162085597.1 2723897..2725024(+) (pilU) [Sulfuriferula nivalis strain SGTM]
ATGGAAGCACTCAAAACTGTACACGACCTGCTCAGACTCATGTTGAGCAAACGCGCATCTGATTTGTTCATCACGGCTGG
TTTTCCCCCTGCCATCAAGGTCGATGGCAAAATCGCGCCTGTATCCAATCAGACACTCACTCCTTCACATACCCGTGAGC
TGGCGCGCAGTATCATGAATGACAGGCAGTGGCATGATTTTGAACATACGCACGAAGCCAATTTTGCTATATCACCTGAG
GGGATAGGTCGCTTCCGTGTGAACGCATTTGTCCAGCAAGGTCGTATAGGTTTAGTCCTGCGTACCATCACCACACTCAT
TCCCAAACTTGAACAACTTAATCTGCCCCCCGTACTCAAGGACGTGGCTATGGTAAAACGCGGTTTGGTTATTTTTGTCG
GTGGTACAGGCTCTGGTAAATCAACATCATTGGCAGCCATGGTTGGGCACCGTAACGAAAATGCCCAGGATCACATCATC
ACCATCGAAGATCCTGTTGAATATGTACACGAACACAAAAAATCCATTATCACTCAGCGTGAAGTCGGCATAGATACCGA
CAACTGGTTCTCAGCCCTCAAAAACACCCTGCGCCAGGCACCTGACGTCATCCTTATCGGTGAGGTACGTGACCGCGAGA
CCATGGAATACGCCATCGCCTTTGCCGAAACAGGTCACCTGTGCTTCACCACGCTACACGCGAACTCGGCCAACCAGGCA
CTGGATCGTATTATCAACTTCTTCCCTGATGAGCGCCGTCAGCAATTGCTCATGGATTTATCACTGAATCTGAAAGCTTT
TGTCTCCCAGCGCCTGATCGCCCGTAAAGGCACAACCGGACGAATTGCCGCTGTTGAAGTATTGCTAAACTCGCCGCTCA
TTTCCGAACTGATATTCAAAGGTGAAGTCCATGAAATCAAGGCGATTATGGCAAAGTCACGGGAAATGGGTATGCAGACT
TTCGATCAATCACTATTTGATTTGTATGAAGCTGGACTGATTACTTATGAAGATGCGCTACGCAATGCCGACTCACTCAA
CGACCTCAGACTGAAAATAAAACTGGAAGGCCATGAAGCCAAAGGGCGTGATGTGATGGATGGAATACAGAGTCTGAATA
TTGTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(122-253)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A809SAV2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.192

97.333

0.605
  pilU Acinetobacter baylyi ADP1

58.939

95.467

0.563
  pilU Vibrio cholerae strain A1552

55.425

90.933

0.504
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.807

89.867

0.403
  pilT Acinetobacter baylyi ADP1

43.068

90.4

0.389
  pilT Pseudomonas aeruginosa PAK

43.027

89.867

0.387
  pilT Acinetobacter nosocomialis M2

42.643

88.8

0.379
  pilT Acinetobacter baumannii strain A118

42.643

88.8

0.379
  pilT Acinetobacter baumannii D1279779

42.643

88.8

0.379
  pilT Vibrio cholerae strain A1552

42.169

88.533

0.373
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.169

88.533

0.373
  pilT Pseudomonas stutzeri DSM 10701

41.543

89.867

0.373
  pilT Legionella pneumophila strain Lp02

41.141

88.8

0.365
  pilT Legionella pneumophila strain ERS1305867

41.141

88.8

0.365

Multiple sequence alignment