Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SMATCC274_RS20485 Genome accession   NZ_AP021873
Coordinates   4330346..4331368 (-) Length   340 a.a.
NCBI ID   WP_048796770.1    Uniprot ID   -
Organism   Serratia marcescens strain ATCC 274     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4325346..4336368
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SMATCC274_RS20455 (SMATCC274_40260) - 4326147..4326659 (+) 513 WP_033639394.1 SprT family zinc-dependent metalloprotease -
  SMATCC274_RS20460 (SMATCC274_40270) endA 4326761..4327456 (+) 696 WP_015379043.1 deoxyribonuclease I -
  SMATCC274_RS20465 (SMATCC274_40280) rsmE 4327526..4328257 (+) 732 WP_004937458.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  SMATCC274_RS20470 (SMATCC274_40290) gshB 4328268..4329218 (+) 951 WP_016930084.1 glutathione synthase -
  SMATCC274_RS20475 (SMATCC274_40300) - 4329364..4329927 (+) 564 WP_033635940.1 YqgE/AlgH family protein -
  SMATCC274_RS20480 (SMATCC274_40310) ruvX 4329927..4330349 (+) 423 WP_015379046.1 Holliday junction resolvase RuvX -
  SMATCC274_RS20485 (SMATCC274_40320) pilT 4330346..4331368 (-) 1023 WP_048796770.1 type IV pilus twitching motility protein PilT Machinery gene
  SMATCC274_RS20490 (SMATCC274_40330) - 4331389..4332096 (+) 708 WP_048796771.1 YggS family pyridoxal phosphate-dependent enzyme -
  SMATCC274_RS20495 (SMATCC274_40340) proC 4332116..4332937 (+) 822 WP_048796772.1 pyrroline-5-carboxylate reductase -
  SMATCC274_RS20500 (SMATCC274_40350) - 4332969..4333523 (+) 555 WP_004937436.1 YggT family protein -
  SMATCC274_RS20505 (SMATCC274_40360) yggU 4333520..4333810 (+) 291 WP_004948014.1 DUF167 family protein YggU -
  SMATCC274_RS20510 (SMATCC274_40370) - 4333829..4334422 (+) 594 WP_004937429.1 XTP/dITP diphosphatase -
  SMATCC274_RS20515 (SMATCC274_40380) hemW 4334415..4335557 (+) 1143 WP_048796774.1 radical SAM family heme chaperone HemW -
  SMATCC274_RS20520 (SMATCC274_40390) - 4335594..4336028 (-) 435 WP_161545044.1 DUF29 domain-containing protein -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36474.75 Da        Isoelectric Point: 7.2889

>NTDB_id=75154 SMATCC274_RS20485 WP_048796770.1 4330346..4331368(-) (pilT) [Serratia marcescens strain ATCC 274]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAPTLTAQGVQALCDGLLNAQQRESLRRLGQVDLALHRPGGE
RLRANVFQQSAGISLALRRIAGQSPSLAELAAPAIVPALLRRDDGLILVTGATGSGKSTTLAAMIDEINRHQQRHVLTLE
DPIEFLHRSRRSLIQQREIGRDSHSFDAALRAALREDPDVILLGELRDTATIRLALTAAETGHLVLATLHTRSAPQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGVLGECAGERAGV

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=75154 SMATCC274_RS20485 WP_048796770.1 4330346..4331368(-) (pilT) [Serratia marcescens strain ATCC 274]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGTCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCGCTGGCGGCGGCGCCAACGCTGACGGCGCAAGGCGTGCAGGCCCTCTGCGACG
GATTGCTGAATGCGCAGCAGCGCGAGAGCCTGCGGCGGCTGGGGCAGGTCGATCTGGCGTTGCACAGGCCGGGCGGGGAG
CGGCTGCGGGCCAACGTTTTTCAACAAAGCGCGGGGATTTCTCTCGCGTTGCGGCGTATCGCCGGGCAATCGCCTTCGCT
TGCCGAGCTGGCGGCGCCGGCCATTGTCCCGGCGTTGCTGCGGCGCGACGACGGGCTTATCTTGGTCACCGGCGCCACCG
GCAGCGGCAAGTCCACCACGTTGGCGGCGATGATCGACGAGATCAACCGGCACCAGCAGCGGCACGTTTTGACGCTGGAG
GATCCGATCGAGTTCCTGCACCGCAGCCGGCGATCGCTGATCCAGCAGCGGGAGATCGGCCGCGACAGCCACAGCTTCGA
TGCGGCGTTGCGCGCCGCGCTGCGCGAAGATCCGGATGTGATCCTGCTGGGCGAGCTGCGCGATACCGCCACCATCCGGC
TGGCGCTCACTGCGGCGGAGACCGGCCACCTGGTGCTGGCGACGCTGCATACCCGCAGCGCGCCGCAGGCGGTGGAACGG
CTGGTGGACGTGTTTCCGGCGGAAGAGAAACCCTATGTGCGCGCCCAGCTGGCCGGCAGTCTGCAGGCGGTGATCGCGCA
AAAGCTGATGAGGCGGCCCGGCGGCGGACGCGTGGCGATCTTCGAAGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGCGAAGGGAAAACGCATCAGTTGGCGAGCGTGTTGCAAACCGGCGCGCAGTCCGGCATGCAAACCTTTGAGCAAGGT
TTGCAGCAGCGGATCGACGCCGGCGTGTTGGGAGAGTGCGCGGGGGAAAGGGCGGGCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

54.545

97.059

0.529
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.545

97.059

0.529
  pilT Neisseria meningitidis 8013

50

100

0.5
  pilT Acinetobacter baylyi ADP1

51.057

97.353

0.497
  pilT Pseudomonas aeruginosa PAK

48.974

100

0.491
  pilT Acinetobacter nosocomialis M2

50.606

97.059

0.491
  pilT Acinetobacter baumannii strain A118

50.606

97.059

0.491
  pilT Acinetobacter baumannii D1279779

50.606

97.059

0.491
  pilT Neisseria gonorrhoeae MS11

49.408

99.412

0.491
  pilT Pseudomonas stutzeri DSM 10701

48.378

99.706

0.482
  pilT Legionella pneumophila strain Lp02

48.235

100

0.482
  pilT Legionella pneumophila strain ERS1305867

48.235

100

0.482
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.737

100

0.45
  pilU Pseudomonas stutzeri DSM 10701

38.348

99.706

0.382
  pilU Vibrio cholerae strain A1552

39.571

95.882

0.379
  pilU Acinetobacter baylyi ADP1

39.938

95

0.379

Multiple sequence alignment