Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   Vgang_RS09615 Genome accession   NZ_AP021869
Coordinates   2085792..2086829 (-) Length   345 a.a.
NCBI ID   WP_105900842.1    Uniprot ID   -
Organism   Vibrio gangliei strain DSM 104291     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2080792..2091829
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Vgang_RS09585 rsmE 2081347..2082078 (+) 732 WP_105900847.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  Vgang_RS09590 gshB 2082142..2083092 (+) 951 WP_105900931.1 glutathione synthase -
  Vgang_RS09595 - 2083154..2083717 (+) 564 WP_105900846.1 YqgE/AlgH family protein -
  Vgang_RS09600 ruvX 2083773..2084195 (+) 423 WP_105900845.1 Holliday junction resolvase RuvX -
  Vgang_RS09605 - 2084212..2084637 (-) 426 WP_105900844.1 DUF3293 domain-containing protein -
  Vgang_RS09610 pilU 2084656..2085762 (-) 1107 WP_105900843.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Vgang_RS09615 pilT 2085792..2086829 (-) 1038 WP_105900842.1 type IV pilus twitching motility protein PilT Machinery gene
  Vgang_RS09620 - 2086855..2087571 (+) 717 WP_105900841.1 YggS family pyridoxal phosphate-dependent enzyme -
  Vgang_RS09625 proC 2087706..2088524 (+) 819 WP_105900840.1 pyrroline-5-carboxylate reductase -
  Vgang_RS09630 - 2088564..2089118 (+) 555 WP_105900839.1 YggT family protein -
  Vgang_RS09635 yggU 2089129..2089443 (+) 315 WP_105900838.1 DUF167 family protein YggU -
  Vgang_RS09640 - 2089440..2089880 (+) 441 WP_105900837.1 DUF4426 domain-containing protein -
  Vgang_RS09645 - 2089899..2090507 (+) 609 WP_105900836.1 XTP/dITP diphosphatase -
  Vgang_RS09650 hemW 2090511..2091686 (+) 1176 WP_170066808.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38535.19 Da        Isoelectric Point: 5.5779

>NTDB_id=75107 Vgang_RS09615 WP_105900842.1 2085792..2086829(-) (pilT) [Vibrio gangliei strain DSM 104291]
MDIAELLDFSVKHNASDLHLSAGVPPMLRIDGDVRKLDMEAFSHSDVHALVFDIMNDAQRSEFEEKLEIDFSFELPNVGR
FRVNVFNQSRGCAAVFRTIPMIIPSFEDIDAPEIFEKISNYEKGLVLVTGPTGSGKSTTLAAMVDYINKHHKKHILTIED
PIEFTHTSYKCLVNQREVHRDTHSFEASLRSALREDPDVILVGELRDKETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGADKDMVRSMLSESLRAVIAQKLLKRSDKGRVACHEVMIATPAIRNLIREDKVAQMLSMIQTGSSIGMQTMEQAA
RQLLLQGLVEKEEVETKIETMASPY

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=75107 Vgang_RS09615 WP_105900842.1 2085792..2086829(-) (pilT) [Vibrio gangliei strain DSM 104291]
ATGGACATCGCTGAGTTATTGGATTTTAGTGTAAAGCATAATGCATCAGATCTACATCTTTCTGCAGGTGTGCCTCCCAT
GTTACGCATTGATGGCGATGTAAGAAAGTTAGATATGGAAGCATTTAGCCATTCTGATGTTCATGCTTTGGTGTTCGATA
TTATGAATGATGCTCAGCGTAGTGAATTTGAAGAAAAGCTTGAAATCGACTTTTCGTTTGAACTACCTAATGTGGGCCGT
TTTCGTGTCAATGTGTTTAACCAGTCTCGTGGTTGCGCAGCGGTTTTTCGTACCATCCCAATGATCATTCCTTCGTTTGA
AGACATTGATGCGCCAGAAATCTTTGAAAAAATTTCCAATTATGAAAAAGGCTTAGTGTTAGTCACTGGCCCAACCGGAT
CGGGTAAATCCACGACCTTGGCGGCGATGGTTGATTACATCAATAAGCACCATAAAAAACATATCTTAACCATTGAAGAT
CCTATCGAATTTACTCATACCAGCTATAAGTGCTTGGTAAACCAGCGTGAAGTTCATCGTGATACCCATAGCTTTGAAGC
CTCGTTACGTTCGGCTTTGCGTGAAGACCCTGATGTGATTTTGGTGGGTGAGCTACGTGATAAAGAAACCATTAGCCTAG
CTTTAACTGCAGCGGAAACGGGTCACTTGGTGTTTGGTACTTTGCATACCAGTTCGGCGGCTAAAACCATCGACCGTATT
ATTGATGTGTTCCCAGGGGCGGATAAAGACATGGTGCGTTCGATGTTGTCTGAATCTTTACGTGCGGTTATTGCTCAAAA
ACTGTTAAAGCGCAGTGATAAAGGCCGTGTAGCCTGCCATGAAGTGATGATTGCCACCCCTGCGATTCGTAACCTGATCC
GTGAAGATAAAGTGGCGCAAATGCTATCGATGATCCAAACGGGCTCGTCAATTGGTATGCAAACCATGGAGCAAGCCGCA
CGTCAGCTATTGCTGCAAGGTTTGGTGGAAAAAGAAGAAGTTGAAACCAAAATTGAAACGATGGCCTCTCCATACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

82.596

98.261

0.812
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

82.596

98.261

0.812
  pilT Pseudomonas stutzeri DSM 10701

68.546

97.681

0.67
  pilT Acinetobacter nosocomialis M2

68.155

97.391

0.664
  pilT Acinetobacter baumannii D1279779

68.155

97.391

0.664
  pilT Acinetobacter baumannii strain A118

68.155

97.391

0.664
  pilT Acinetobacter baylyi ADP1

69.301

95.362

0.661
  pilT Pseudomonas aeruginosa PAK

67.359

97.681

0.658
  pilT Legionella pneumophila strain Lp02

67.372

95.942

0.646
  pilT Legionella pneumophila strain ERS1305867

67.372

95.942

0.646
  pilT Neisseria meningitidis 8013

63.363

96.522

0.612
  pilT Neisseria gonorrhoeae MS11

63.063

96.522

0.609
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.572

92.174

0.475
  pilU Pseudomonas stutzeri DSM 10701

42.216

96.812

0.409
  pilU Vibrio cholerae strain A1552

42.945

94.493

0.406
  pilU Acinetobacter baylyi ADP1

40.81

93.043

0.38

Multiple sequence alignment