Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   Vgang_RS09610 Genome accession   NZ_AP021869
Coordinates   2084656..2085762 (-) Length   368 a.a.
NCBI ID   WP_105900843.1    Uniprot ID   -
Organism   Vibrio gangliei strain DSM 104291     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2079656..2090762
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Vgang_RS09575 - 2079758..2080240 (+) 483 WP_406708308.1 SprT family zinc-dependent metalloprotease -
  Vgang_RS09580 - 2080335..2081216 (+) 882 WP_105900848.1 GGDEF domain-containing protein -
  Vgang_RS09585 rsmE 2081347..2082078 (+) 732 WP_105900847.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  Vgang_RS09590 gshB 2082142..2083092 (+) 951 WP_105900931.1 glutathione synthase -
  Vgang_RS09595 - 2083154..2083717 (+) 564 WP_105900846.1 YqgE/AlgH family protein -
  Vgang_RS09600 ruvX 2083773..2084195 (+) 423 WP_105900845.1 Holliday junction resolvase RuvX -
  Vgang_RS09605 - 2084212..2084637 (-) 426 WP_105900844.1 DUF3293 domain-containing protein -
  Vgang_RS09610 pilU 2084656..2085762 (-) 1107 WP_105900843.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Vgang_RS09615 pilT 2085792..2086829 (-) 1038 WP_105900842.1 type IV pilus twitching motility protein PilT Machinery gene
  Vgang_RS09620 - 2086855..2087571 (+) 717 WP_105900841.1 YggS family pyridoxal phosphate-dependent enzyme -
  Vgang_RS09625 proC 2087706..2088524 (+) 819 WP_105900840.1 pyrroline-5-carboxylate reductase -
  Vgang_RS09630 - 2088564..2089118 (+) 555 WP_105900839.1 YggT family protein -
  Vgang_RS09635 yggU 2089129..2089443 (+) 315 WP_105900838.1 DUF167 family protein YggU -
  Vgang_RS09640 - 2089440..2089880 (+) 441 WP_105900837.1 DUF4426 domain-containing protein -
  Vgang_RS09645 - 2089899..2090507 (+) 609 WP_105900836.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41202.19 Da        Isoelectric Point: 5.8006

>NTDB_id=75106 Vgang_RS09610 WP_105900843.1 2084656..2085762(-) (pilU) [Vibrio gangliei strain DSM 104291]
MQLSQVLEIMVSSKASDIYLTVDAPCLYRINGELKAHGEPLDRSGVEALLFEMMDAERQAEFQTTHEANFAIVSSSGRFR
VSAFFQRELPGAVIRRIETRIPTMEELSLPEVLKDLASAKRGLVLVVGATGSGKSTSMAAMTGYRNLTQTGHILTVEDPI
EFVHEHKKCIVTQREVGLDTESYEIALKNSLRQAPDMILIGEIRNRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSLNLKGVVAQQLVRDKNDNGRHGVFEVLLNTPRVSDLIRRGELHELKTTMMRSNESGMQTFDQALF
NLLQQDKITLDDAMHCADSANDLRMMLKSSGTGSSFETSKLKDIKIDL

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=75106 Vgang_RS09610 WP_105900843.1 2084656..2085762(-) (pilU) [Vibrio gangliei strain DSM 104291]
ATGCAGTTGTCTCAAGTATTAGAAATCATGGTGTCTTCGAAAGCATCGGATATTTATTTAACGGTTGATGCACCATGTTT
ATATCGCATTAATGGCGAGCTTAAAGCACATGGTGAGCCTTTAGATCGTAGTGGGGTAGAAGCTCTGTTATTTGAAATGA
TGGATGCTGAGCGACAAGCCGAATTTCAAACAACCCATGAAGCGAACTTTGCCATTGTAAGTTCTAGTGGACGCTTTCGT
GTCAGTGCCTTTTTCCAACGAGAATTACCTGGTGCGGTGATTCGTCGCATTGAAACGCGCATTCCTACGATGGAAGAGCT
GAGCCTACCCGAAGTACTGAAAGATCTGGCTAGTGCCAAACGTGGTTTAGTGCTGGTGGTTGGGGCGACCGGTTCGGGTA
AATCAACGTCGATGGCGGCGATGACAGGGTATCGTAACTTAACTCAAACCGGCCATATTTTGACGGTAGAGGACCCCATT
GAATTTGTGCATGAGCATAAAAAATGCATCGTCACACAACGTGAAGTGGGGCTTGATACAGAAAGCTATGAAATTGCGTT
GAAAAATTCATTACGCCAAGCGCCAGATATGATTTTGATTGGTGAAATTCGTAACCGTGAAACCATGGAATACGCCATGA
CCTTTGCGGAAACGGGGCATTTATGTATGGCGACCTTGCACGCCAACAACGCCAACCAAGCGCTTGAACGTATTTTGCAT
TTAGTGCCGAAAGAGCAAAAAGAGCAATTCTTGTTTGATTTATCACTAAACTTAAAAGGTGTGGTCGCTCAGCAATTAGT
GCGAGATAAAAATGACAATGGTCGTCACGGGGTATTTGAAGTCTTACTTAATACACCTAGGGTGTCGGATTTGATTCGCC
GTGGTGAATTGCATGAGTTGAAAACAACCATGATGCGTTCAAATGAATCAGGTATGCAGACCTTTGACCAAGCGTTATTT
AATTTATTACAGCAAGATAAAATCACCTTGGATGATGCGATGCATTGCGCAGATTCTGCCAATGATTTGCGTATGATGTT
GAAGTCTTCTGGTACTGGCTCGAGCTTTGAAACTAGTAAGTTAAAAGACATTAAAATCGATTTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

77.446

100

0.774
  pilU Pseudomonas stutzeri DSM 10701

58

95.109

0.552
  pilU Acinetobacter baylyi ADP1

53.541

95.924

0.514
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.773

92.12

0.394
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.936

92.935

0.38
  pilT Vibrio cholerae strain A1552

40.936

92.935

0.38
  pilT Legionella pneumophila strain ERS1305867

41.298

92.12

0.38
  pilT Legionella pneumophila strain Lp02

41.298

92.12

0.38
  pilT Acinetobacter baylyi ADP1

40.176

92.663

0.372
  pilT Pseudomonas stutzeri DSM 10701

40.299

91.033

0.367
  pilT Pseudomonas aeruginosa PAK

40.299

91.033

0.367

Multiple sequence alignment