Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CLAM6_RS01005 Genome accession   NZ_AP021868
Coordinates   214375..215406 (+) Length   343 a.a.
NCBI ID   WP_153635076.1    Uniprot ID   A0A5K7Y1N0
Organism   Cobetia sp. AM6     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 209375..220406
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CLAM6_RS00980 (CLAM6_01940) metW 209712..210326 (-) 615 WP_153635072.1 methionine biosynthesis protein MetW -
  CLAM6_RS00985 (CLAM6_01950) - 210319..211512 (-) 1194 WP_153635073.1 homoserine O-acetyltransferase -
  CLAM6_RS00990 (CLAM6_01960) - 211782..212363 (-) 582 WP_043333437.1 YggT family protein -
  CLAM6_RS00995 (CLAM6_01970) proC 212513..213355 (-) 843 WP_153635074.1 pyrroline-5-carboxylate reductase -
  CLAM6_RS01000 (CLAM6_01980) - 213427..214179 (-) 753 WP_153635075.1 YggS family pyridoxal phosphate-dependent enzyme -
  CLAM6_RS01005 (CLAM6_01990) pilT 214375..215406 (+) 1032 WP_153635076.1 type IV pilus twitching motility protein PilT Machinery gene
  CLAM6_RS01010 (CLAM6_02000) pilU 215546..216700 (+) 1155 WP_043333447.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CLAM6_RS01015 (CLAM6_02010) - 216807..217955 (-) 1149 WP_043333449.1 anhydro-N-acetylmuramic acid kinase -
  CLAM6_RS01020 (CLAM6_02020) tyrS 218111..219319 (+) 1209 WP_043333452.1 tyrosine--tRNA ligase -

Sequence

Protein


Download         Length: 343 a.a.        Molecular weight: 37739.36 Da        Isoelectric Point: 6.0550

>NTDB_id=75068 CLAM6_RS01005 WP_153635076.1 214375..215406(+) (pilT) [Cobetia sp. AM6]
MDITELLAFSAKQNASDLHLSAGLPPMIRVDGDVRRINVPSMDDKQVRALIYEIMNDKQRRDFEEFLETDFSFEVPGVSR
FRVNAFHQGRGAGAVFRTIPSEVLTMEDLGLGEAFRKMCMLPRGLILVTGPTGSGKSTTLAAMVDYINENKYDHILTIED
PVEFVHQSKRCLVNQREVHRDTHGFAPALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTTSATKTVDRI
IDVFPGEEKSMVRSMLSESLQGVIAQTLLKKIGGGRVAAHEIMIATPAIRNLIREDKVAQMYSSIQTGGALGMQTLDAAL
TRLLAEGLVTNEDAQAKAKGLLN

Nucleotide


Download         Length: 1032 bp        

>NTDB_id=75068 CLAM6_RS01005 WP_153635076.1 214375..215406(+) (pilT) [Cobetia sp. AM6]
ATGGATATTACCGAACTGCTGGCTTTTTCGGCAAAACAGAACGCGTCGGATCTTCACCTGTCCGCTGGTTTGCCACCGAT
GATTCGTGTCGACGGTGATGTGCGGCGCATCAACGTGCCGTCAATGGATGACAAGCAGGTGCGCGCGCTGATCTACGAGA
TCATGAACGACAAGCAGCGCCGTGACTTCGAGGAATTCCTCGAGACCGATTTCTCCTTCGAGGTGCCGGGTGTCTCGCGC
TTTCGTGTCAACGCCTTCCATCAGGGGCGTGGCGCGGGGGCCGTCTTCCGTACCATCCCCTCGGAAGTGCTGACGATGGA
GGATCTGGGGCTCGGTGAAGCCTTCCGCAAGATGTGCATGCTGCCGCGCGGCCTGATTCTTGTCACCGGGCCGACCGGCT
CCGGTAAATCCACCACGCTGGCGGCGATGGTCGATTACATCAACGAGAACAAGTACGACCACATCCTCACCATCGAGGAC
CCGGTGGAATTCGTGCACCAGTCCAAGCGATGTCTGGTCAACCAGCGTGAAGTGCACCGTGATACCCACGGCTTCGCGCC
CGCGCTGCGTTCGGCGCTGCGTGAAGACCCGGATGTCATTCTGGTCGGCGAGCTGCGAGATCTCGAGACCATCCGCCTGG
CGCTGACCGCGGCCGAGACGGGCCACCTGGTGTTCGGCACCCTGCACACCACCTCCGCGACCAAGACCGTCGACCGTATC
ATCGACGTCTTCCCCGGCGAGGAGAAGTCGATGGTGCGCTCGATGCTGTCCGAGTCCCTGCAGGGCGTCATCGCCCAGAC
ACTGCTCAAGAAGATCGGCGGTGGCCGAGTGGCGGCTCACGAGATCATGATCGCGACGCCTGCCATTCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCCTCCATCCAGACCGGTGGCGCCCTGGGCATGCAGACACTGGATGCAGCGCTG
ACGCGTCTGTTGGCGGAAGGGCTGGTGACCAATGAAGATGCTCAGGCGAAAGCCAAGGGCTTGTTGAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5K7Y1N0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

84.071

98.834

0.831
  pilT Pseudomonas aeruginosa PAK

82.301

98.834

0.813
  pilT Acinetobacter baumannii D1279779

78.466

98.834

0.776
  pilT Acinetobacter baumannii strain A118

78.466

98.834

0.776
  pilT Acinetobacter nosocomialis M2

78.171

98.834

0.773
  pilT Acinetobacter baylyi ADP1

77.876

98.834

0.77
  pilT Legionella pneumophila strain Lp02

72.781

98.542

0.717
  pilT Legionella pneumophila strain ERS1305867

72.781

98.542

0.717
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.326

98.251

0.691
  pilT Vibrio cholerae strain A1552

70.326

98.251

0.691
  pilT Neisseria meningitidis 8013

65.689

99.417

0.653
  pilT Neisseria gonorrhoeae MS11

65.396

99.417

0.65
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.327

98.834

0.507
  pilU Pseudomonas stutzeri DSM 10701

42.985

97.668

0.42
  pilU Vibrio cholerae strain A1552

41.194

97.668

0.402
  pilU Acinetobacter baylyi ADP1

39.412

99.125

0.391

Multiple sequence alignment