Detailed information NTG0075069

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: CLAM6_RS01010
• Genome accession: NZ_AP021868
• Coordinates: 215546..216700 (+)
• Length: 384 a.a.
• NCBI ID: WP_043333447.1
• Uniprot ID: A0AAP4TZ60
• Organism: Cobetia sp. AM6
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 210546..221700

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CLAM6_RS00990 (CLAM6_01960)	-	211782..212363 (-)	582	WP_043333437.1	YggT family protein	-
CLAM6_RS00995 (CLAM6_01970)	proC	212513..213355 (-)	843	WP_153635074.1	pyrroline-5-carboxylate reductase	-
CLAM6_RS01000 (CLAM6_01980)	-	213427..214179 (-)	753	WP_153635075.1	YggS family pyridoxal phosphate-dependent enzyme	-
CLAM6_RS01005 (CLAM6_01990)	pilT	214375..215406 (+)	1032	WP_153635076.1	type IV pilus twitching motility protein PilT	Machinery gene
CLAM6_RS01010 (CLAM6_02000)	pilU	215546..216700 (+)	1155	WP_043333447.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
CLAM6_RS01015 (CLAM6_02010)	-	216807..217955 (-)	1149	WP_043333449.1	anhydro-N-acetylmuramic acid kinase	-
CLAM6_RS01020 (CLAM6_02020)	tyrS	218111..219319 (+)	1209	WP_043333452.1	tyrosine--tRNA ligase	-

Sequence

Protein

• Length: 384 a.a.
• Molecular weight: 43054.35 Da
• Isoelectric Point: 6.3429

>NTDB_id=75069 CLAM6_RS01010 WP_043333447.1 215546..216700(+) (pilU) [Cobetia sp. AM6]
MTPHEWLHELLQLMVSKGSSDLFISTGTPPQMKVNGRMVALGDKKLSVDQVRELVLAPMSDMQRERFEEEREANFAHSLP
GVGRFRISAFYQRSQMGMVIRRIQLSIPSLEELRLPEIIKGLSETKRGLVIFVGGTGAGKSTSLAAMIQHRNQTSSGHII
CIEDPIEYIHPHQRSIVTQREVGIDTESFEVALRNTLRQAPDVIMIGEIRSRETMEHALTFAETGHLCLATLHANNANQA
LDRIIHFFPEERHEQVWMDLSLNLKGIVAQQLLPHKSGNTTQRVPAIEVMLRSPLIVDLIRKGAVVEIKDVMKRSQQQGM
MTFDQSLYALHQQGLITEEVALAHADSANDLRLMIKFGDSDSAQEAQLDVLNAASRFSLQGDDD

Nucleotide

• Length: 1155 bp

>NTDB_id=75069 CLAM6_RS01010 WP_043333447.1 215546..216700(+) (pilU) [Cobetia sp. AM6]
ATGACTCCTCATGAATGGCTGCATGAACTTCTGCAGTTGATGGTCAGCAAGGGCAGCTCGGACCTGTTCATCTCCACCGG
CACACCGCCGCAGATGAAGGTCAATGGCCGCATGGTGGCGCTGGGTGACAAGAAGCTGAGTGTCGATCAGGTGAGAGAGC
TGGTGCTCGCACCGATGAGTGACATGCAGCGAGAGCGCTTCGAGGAAGAGCGCGAGGCCAACTTCGCGCATAGCTTGCCG
GGAGTCGGACGTTTCAGGATTAGCGCCTTCTACCAGCGCAGCCAGATGGGCATGGTGATTCGCCGCATCCAGCTGTCGAT
TCCCAGCCTGGAAGAGTTGCGGCTGCCGGAGATCATCAAGGGGCTTTCCGAGACCAAGCGTGGTCTGGTGATCTTCGTCG
GCGGTACCGGCGCCGGCAAGTCGACCTCGCTGGCGGCGATGATCCAGCACCGCAACCAGACCTCGAGCGGGCATATCATC
TGCATCGAAGACCCGATTGAGTATATCCACCCCCACCAGCGCTCCATCGTGACGCAGCGTGAAGTGGGGATCGATACCGA
GTCCTTCGAGGTGGCACTGCGCAATACGCTGCGTCAGGCGCCGGACGTGATCATGATCGGTGAGATCCGCTCGCGTGAGA
CCATGGAGCACGCGCTGACCTTCGCCGAGACCGGTCACCTGTGTCTTGCGACGTTGCATGCCAACAATGCCAACCAGGCG
CTGGACCGCATCATCCACTTCTTCCCGGAGGAGCGTCATGAACAGGTGTGGATGGATCTGTCGCTGAACCTCAAGGGCAT
CGTGGCCCAGCAGCTGTTGCCTCACAAGAGTGGCAACACCACCCAGCGCGTGCCGGCCATTGAGGTGATGCTGCGTTCGC
CGCTGATCGTCGACCTGATCCGCAAGGGCGCGGTGGTCGAGATCAAGGACGTCATGAAGCGCTCGCAGCAGCAGGGCATG
ATGACTTTCGATCAATCGCTGTACGCCTTGCATCAGCAGGGCTTGATCACCGAGGAAGTGGCATTGGCGCATGCCGATTC
CGCCAATGACCTGCGCCTGATGATCAAGTTCGGTGATTCCGACAGCGCCCAGGAAGCCCAGCTGGATGTCCTGAATGCCG
CCAGTCGCTTCTCGCTGCAAGGGGATGACGACTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (84-273)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	65.625	91.667	0.602
pilU	Vibrio cholerae strain A1552	56.64	96.094	0.544
pilU	Acinetobacter baylyi ADP1	55.932	92.188	0.516
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	44.348	89.844	0.398
pilT	Neisseria meningitidis 8013	40.816	89.323	0.365
pilT	Pseudomonas aeruginosa PAK	41.742	86.719	0.362