Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GDK41_RS02430 Genome accession   NZ_AP021846
Coordinates   519561..520604 (+) Length   347 a.a.
NCBI ID   WP_152084920.1    Uniprot ID   A0A5H2YJF1
Organism   Pseudoalteromonas sp. A25     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 514561..525604
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GDK41_RS02400 (PA25_04600) hemW 515273..516409 (-) 1137 WP_152084914.1 radical SAM family heme chaperone HemW -
  GDK41_RS02405 (PA25_04610) - 516406..516999 (-) 594 WP_152084915.1 XTP/dITP diphosphatase -
  GDK41_RS02410 (PA25_04620) - 517006..517443 (-) 438 WP_152084916.1 DUF4426 domain-containing protein -
  GDK41_RS02415 (PA25_04630) - 517455..517988 (-) 534 WP_152084917.1 YggT family protein -
  GDK41_RS02420 (PA25_04640) proC 517999..518820 (-) 822 WP_152084918.1 pyrroline-5-carboxylate reductase -
  GDK41_RS02425 (PA25_04650) - 518835..519530 (-) 696 WP_152084919.1 YggS family pyridoxal phosphate-dependent enzyme -
  GDK41_RS02430 (PA25_04660) pilT 519561..520604 (+) 1044 WP_152084920.1 type IV pilus twitching motility protein PilT Machinery gene
  GDK41_RS02435 (PA25_04670) pilU 520617..521732 (+) 1116 WP_152084921.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GDK41_RS02440 (PA25_04680) - 521806..522591 (+) 786 WP_152084922.1 hypothetical protein -
  GDK41_RS02445 (PA25_04690) ruvX 522593..523039 (-) 447 WP_152084923.1 Holliday junction resolvase RuvX -
  GDK41_RS02450 (PA25_04700) - 523048..523605 (-) 558 WP_152084924.1 YqgE/AlgH family protein -
  GDK41_RS02455 (PA25_04710) gshB 523628..524584 (-) 957 WP_152084925.1 glutathione synthase -
  GDK41_RS02460 (PA25_04720) rsmE 524635..525369 (-) 735 WP_152087489.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38633.86 Da        Isoelectric Point: 6.3163

>NTDB_id=74949 GDK41_RS02430 WP_152084920.1 519561..520604(+) (pilT) [Pseudoalteromonas sp. A25]
MDITELLAFSVQHQASDLHLSSGVSPMIRVDGDVRRVNIPALEAKDVNSLVYDIMNDNQRRDYEQNLEVDFSFEVPNLAR
FRVNAFNTNRGPAAVFRTIPSSVLSLDDLGAPDIFRKIAEYRKGLVLVTGPTGSGKSTTLAALVDHINATQHYHILTIED
PIEFVHKNQKSLINQREVHRDTHSFNAALRSALREDPDVILVGELRDLETIRLAMTAAETGHLVFGTLHTTSAPKTIDRI
IDVFPGEEKDMVRSMLSESLQAVISQTLVRKVSGGRIAAHEIMLGTPAIRNLIREDKIPQMYSTIQTGAIHGMQTMEQCL
SNLVNRGVISQQDIPGKGHDEQNQRNF

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=74949 GDK41_RS02430 WP_152084920.1 519561..520604(+) (pilT) [Pseudoalteromonas sp. A25]
ATGGATATTACTGAGTTATTGGCTTTTAGTGTGCAACACCAAGCCTCCGATTTACATTTATCGTCTGGCGTATCGCCAAT
GATACGCGTTGATGGCGATGTGCGCCGCGTTAATATTCCTGCGCTTGAAGCTAAGGATGTTAACAGTCTTGTATACGATA
TCATGAATGATAACCAGCGCAGAGACTATGAGCAAAATCTTGAAGTGGATTTTTCCTTTGAAGTCCCTAATTTGGCGCGA
TTTCGTGTTAATGCCTTTAATACTAACCGTGGCCCTGCTGCGGTGTTTCGAACTATCCCATCATCGGTATTGAGTTTGGA
CGACCTAGGCGCACCCGATATTTTTCGCAAAATAGCAGAGTATAGAAAAGGTTTAGTGTTGGTTACAGGCCCTACGGGCT
CTGGTAAGTCGACTACTTTGGCCGCTTTGGTTGATCATATCAATGCAACACAACATTACCATATACTGACCATTGAAGAT
CCCATCGAGTTTGTCCACAAAAATCAGAAAAGTTTAATAAACCAAAGAGAAGTACATAGAGACACTCATAGCTTTAATGC
AGCGCTTAGAAGTGCCTTACGTGAAGACCCCGATGTTATTTTGGTCGGGGAATTGCGAGATTTGGAAACCATCCGTCTAG
CCATGACCGCTGCTGAAACGGGTCATTTGGTATTTGGCACGCTACACACCACATCGGCACCCAAAACGATAGACCGTATT
ATTGATGTTTTTCCTGGTGAAGAGAAAGACATGGTTCGTTCAATGCTTTCGGAGTCGTTACAAGCGGTTATCTCGCAAAC
ATTAGTTAGAAAAGTCAGCGGTGGTCGGATTGCTGCGCATGAAATTATGCTAGGGACCCCAGCGATACGAAACCTGATCC
GAGAGGATAAAATCCCGCAAATGTACTCAACAATTCAAACGGGTGCGATCCATGGTATGCAAACGATGGAGCAATGTTTA
AGTAACCTTGTCAATAGAGGGGTTATATCTCAGCAGGATATTCCCGGTAAGGGACACGATGAACAAAACCAGAGGAACTT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5H2YJF1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.108

95.677

0.738
  pilT Acinetobacter baumannii D1279779

77.108

95.677

0.738
  pilT Acinetobacter baumannii strain A118

77.108

95.677

0.738
  pilT Acinetobacter baylyi ADP1

75.455

95.101

0.718
  pilT Pseudomonas stutzeri DSM 10701

72.303

98.847

0.715
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.107

97.118

0.7
  pilT Vibrio cholerae strain A1552

72.107

97.118

0.7
  pilT Pseudomonas aeruginosa PAK

70.845

98.847

0.7
  pilT Legionella pneumophila strain ERS1305867

72.205

95.389

0.689
  pilT Legionella pneumophila strain Lp02

72.205

95.389

0.689
  pilT Neisseria gonorrhoeae MS11

69.789

95.389

0.666
  pilT Neisseria meningitidis 8013

69.789

95.389

0.666
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.266

95.389

0.499
  pilU Vibrio cholerae strain A1552

40.419

96.254

0.389
  pilU Pseudomonas stutzeri DSM 10701

39.521

96.254

0.38
  pilU Acinetobacter baylyi ADP1

38.806

96.542

0.375

Multiple sequence alignment