Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   GDK41_RS02435 Genome accession   NZ_AP021846
Coordinates   520617..521732 (+) Length   371 a.a.
NCBI ID   WP_152084921.1    Uniprot ID   -
Organism   Pseudoalteromonas sp. A25     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 515617..526732
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GDK41_RS02405 (PA25_04610) - 516406..516999 (-) 594 WP_152084915.1 XTP/dITP diphosphatase -
  GDK41_RS02410 (PA25_04620) - 517006..517443 (-) 438 WP_152084916.1 DUF4426 domain-containing protein -
  GDK41_RS02415 (PA25_04630) - 517455..517988 (-) 534 WP_152084917.1 YggT family protein -
  GDK41_RS02420 (PA25_04640) proC 517999..518820 (-) 822 WP_152084918.1 pyrroline-5-carboxylate reductase -
  GDK41_RS02425 (PA25_04650) - 518835..519530 (-) 696 WP_152084919.1 YggS family pyridoxal phosphate-dependent enzyme -
  GDK41_RS02430 (PA25_04660) pilT 519561..520604 (+) 1044 WP_152084920.1 type IV pilus twitching motility protein PilT Machinery gene
  GDK41_RS02435 (PA25_04670) pilU 520617..521732 (+) 1116 WP_152084921.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GDK41_RS02440 (PA25_04680) - 521806..522591 (+) 786 WP_152084922.1 hypothetical protein -
  GDK41_RS02445 (PA25_04690) ruvX 522593..523039 (-) 447 WP_152084923.1 Holliday junction resolvase RuvX -
  GDK41_RS02450 (PA25_04700) - 523048..523605 (-) 558 WP_152084924.1 YqgE/AlgH family protein -
  GDK41_RS02455 (PA25_04710) gshB 523628..524584 (-) 957 WP_152084925.1 glutathione synthase -
  GDK41_RS02460 (PA25_04720) rsmE 524635..525369 (-) 735 WP_152087489.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  GDK41_RS02465 (PA25_04730) - 525475..525948 (-) 474 WP_152084926.1 hypothetical protein -

Sequence

Protein


Download         Length: 371 a.a.        Molecular weight: 41205.48 Da        Isoelectric Point: 6.5054

>NTDB_id=74950 GDK41_RS02435 WP_152084921.1 520617..521732(+) (pilU) [Pseudoalteromonas sp. A25]
MLDKYLQLMVDKHASDLFVSAGLPISVKVDGELRPMEDGPIDAAKSLELVESIMTDKQKTQFHEHKECNFAIVTALGRFR
VSAFWQRDCAGMVIRRIITEIPDVKDLGLPSVLTDVIMSKRGLVLFVGGTGTGKSTSLAALLGYRNRNQRGHILTIEDPI
EFVHEHKKSIITQREVGLDTETFEAALKSSLRQAPDVILIGEIRSQETMEYALSFAETGHLCVATLHANNANQAIDRIMH
LVPKEKHDKLKYDLALNLRAIVAQQLIPSSKGEGREAAIEILLNTPLVAELIKKGDVGSIKEAMAKSKEMGMQTFDQALF
ELYKQQRINYADALHHADSPNDLRLMIKLQNNEQQGAGFLQGVTVDGLDKQ

Nucleotide


Download         Length: 1116 bp        

>NTDB_id=74950 GDK41_RS02435 WP_152084921.1 520617..521732(+) (pilU) [Pseudoalteromonas sp. A25]
ATGTTAGATAAATACTTGCAATTGATGGTCGATAAACACGCCTCTGATCTTTTTGTATCTGCAGGGCTGCCTATTAGTGT
GAAAGTAGACGGTGAGCTACGCCCTATGGAAGATGGGCCGATAGATGCCGCTAAATCGCTCGAGTTAGTTGAGTCGATTA
TGACGGATAAGCAAAAGACACAATTTCACGAGCATAAAGAATGTAACTTTGCGATAGTAACAGCATTGGGGCGTTTTAGA
GTTTCGGCTTTTTGGCAAAGAGATTGTGCGGGGATGGTGATCCGCCGCATCATAACTGAAATTCCAGATGTTAAAGACTT
AGGCTTACCTTCGGTATTAACCGATGTGATAATGTCAAAAAGAGGTTTAGTGTTATTTGTTGGTGGCACAGGCACGGGTA
AATCGACATCTTTGGCGGCTTTGCTTGGTTATCGCAATCGCAATCAACGCGGGCATATTCTGACGATTGAAGATCCGATT
GAATTTGTTCATGAGCATAAGAAAAGCATTATTACTCAACGTGAGGTTGGCTTAGACACTGAAACATTTGAAGCAGCATT
GAAAAGCTCTTTGCGCCAAGCGCCGGATGTTATTTTGATTGGAGAAATTCGTTCTCAAGAAACCATGGAATATGCGTTGA
GTTTTGCTGAAACAGGACACTTATGCGTGGCAACCTTACATGCCAACAATGCAAACCAAGCTATTGACCGCATAATGCAT
TTAGTACCCAAAGAGAAGCACGACAAGTTAAAATATGATTTGGCGCTTAATTTGCGTGCCATTGTGGCCCAGCAGCTCAT
CCCGTCGTCAAAAGGCGAGGGGCGAGAAGCTGCTATTGAAATTTTACTCAATACCCCATTGGTTGCAGAGCTAATTAAAA
AAGGGGATGTTGGCTCTATCAAAGAGGCGATGGCAAAATCAAAAGAAATGGGTATGCAAACGTTTGATCAGGCGCTGTTC
GAACTGTACAAGCAACAACGTATTAATTATGCCGATGCGCTGCACCATGCTGACTCTCCAAATGACTTGAGGTTGATGAT
TAAGCTGCAAAATAATGAACAGCAAGGGGCAGGATTTTTGCAAGGGGTTACGGTTGATGGGCTCGATAAGCAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

57.951

100

0.58
  pilU Acinetobacter baylyi ADP1

55.733

100

0.563
  pilU Vibrio cholerae strain A1552

56.712

98.383

0.558
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.539

95.957

0.418
  pilT Acinetobacter baylyi ADP1

42.941

91.644

0.394
  pilT Acinetobacter nosocomialis M2

42.059

91.644

0.385
  pilT Acinetobacter baumannii D1279779

42.059

91.644

0.385
  pilT Acinetobacter baumannii strain A118

42.059

91.644

0.385
  pilT Pseudomonas aeruginosa PAK

41.691

92.453

0.385
  pilT Pseudomonas stutzeri DSM 10701

42.296

89.218

0.377
  pilT Legionella pneumophila strain ERS1305867

42.769

87.601

0.375
  pilT Legionella pneumophila strain Lp02

42.769

87.601

0.375
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.131

84.367

0.364
  pilT Vibrio cholerae strain A1552

43.131

84.367

0.364

Multiple sequence alignment