Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HVMH_RS02535 Genome accession   NZ_AP020335
Coordinates   542610..543647 (-) Length   345 a.a.
NCBI ID   WP_029907579.1    Uniprot ID   -
Organism   Hydrogenovibrio marinus strain MH-110     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 537610..548647
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HVMH_RS02515 (HVMH_0496) - 538405..539241 (+) 837 WP_155837708.1 tetratricopeptide repeat protein -
  HVMH_RS02520 (HVMH_0497) - 539234..540385 (+) 1152 WP_051623158.1 murein L,D-transpeptidase family protein -
  HVMH_RS02525 (HVMH_0498) - 540523..541449 (-) 927 WP_035629127.1 M14 family zinc carboxypeptidase -
  HVMH_RS02530 (HVMH_0499) pilU 541454..542605 (-) 1152 WP_051623159.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HVMH_RS02535 (HVMH_0500) pilT 542610..543647 (-) 1038 WP_029907579.1 type IV pilus twitching motility protein PilT Machinery gene
  HVMH_RS02540 (HVMH_0501) - 543687..544145 (-) 459 WP_035629133.1 DNA polymerase III subunit chi -
  HVMH_RS02545 (HVMH_0502) - 544178..545659 (-) 1482 WP_029907583.1 leucyl aminopeptidase -
  HVMH_RS02550 (HVMH_0503) lptF 545791..546909 (+) 1119 WP_051623160.1 LPS export ABC transporter permease LptF -
  HVMH_RS02555 (HVMH_0504) lptG 546914..548038 (+) 1125 WP_029907588.1 LPS export ABC transporter permease LptG -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38349.89 Da        Isoelectric Point: 5.8462

>NTDB_id=74882 HVMH_RS02535 WP_029907579.1 542610..543647(-) (pilT) [Hydrogenovibrio marinus strain MH-110]
MDITQLLEFSVQQSASDLHLSSGMPPMLRKDGDIQKLDTEHLTDESVKSLVYEVMNDLQRKQFEEYLEVDFSFELPNVAR
FRVNVFQQNRGLGAVFRTIPSTVLSLDAIHAPEIFKSIAQMPRGLVLVTGPTGSGKSTTLAAMVDYINATENVHILTIED
PIEFVHQSKNALINQREVHRDTKSFSNALRSALREDPDVILVGELRDLETISLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDIFPAEEKSMVRSMLSESLQAVISQTLLKRVNGGRVAAHEIMLGSSAIRNLIREDKVPQMYSMIQTSNKVGMKTLDQDL
KQLVNNGIISKDEARSKAVNKADFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=74882 HVMH_RS02535 WP_029907579.1 542610..543647(-) (pilT) [Hydrogenovibrio marinus strain MH-110]
ATGGATATTACCCAACTTTTAGAATTTTCCGTCCAACAAAGCGCCTCTGACCTGCACCTTTCATCAGGCATGCCACCCAT
GTTGCGAAAAGATGGCGATATCCAAAAACTCGATACTGAGCATTTAACCGACGAATCCGTCAAATCGTTGGTATATGAGG
TGATGAACGACCTCCAACGCAAACAGTTTGAAGAATACTTGGAAGTCGACTTCTCTTTTGAGCTGCCGAATGTTGCGCGC
TTCCGCGTCAACGTTTTCCAACAGAATCGTGGACTGGGCGCTGTTTTTCGTACCATCCCTAGCACCGTGTTGTCACTGGA
TGCCATCCATGCGCCGGAAATATTCAAGAGCATTGCACAGATGCCACGAGGACTGGTGTTGGTGACAGGGCCGACCGGCT
CAGGGAAATCGACAACATTGGCTGCAATGGTCGACTACATCAACGCCACGGAAAATGTGCACATCCTGACCATTGAAGAC
CCTATTGAATTCGTTCACCAATCCAAGAATGCCTTAATCAATCAACGGGAAGTACATCGCGACACCAAAAGTTTCAGCAA
TGCATTGCGCTCGGCACTGCGTGAAGACCCCGATGTTATCTTGGTCGGAGAGCTACGCGACCTGGAAACCATCAGTTTGG
CGATGACTGCTGCGGAAACCGGGCACTTGGTGTTCGGCACACTGCACACCAGCTCTGCAGCCAAAACCATTGATCGCATT
ATTGATATCTTCCCTGCCGAAGAAAAATCCATGGTGCGATCCATGCTCTCTGAATCTCTACAGGCCGTCATTTCACAAAC
CCTTTTGAAGCGAGTGAACGGTGGTCGTGTTGCTGCCCATGAAATCATGCTCGGCTCCTCGGCAATTCGCAATTTGATTC
GTGAAGACAAAGTGCCTCAAATGTATTCGATGATTCAGACCTCAAATAAAGTCGGCATGAAAACATTGGATCAGGATTTG
AAGCAATTGGTGAATAACGGCATTATCAGCAAAGATGAAGCGCGCAGTAAAGCCGTCAACAAAGCGGATTTCTTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

71.221

99.71

0.71
  pilT Acinetobacter nosocomialis M2

70.725

100

0.707
  pilT Acinetobacter baumannii D1279779

70.725

100

0.707
  pilT Acinetobacter baumannii strain A118

70.725

100

0.707
  pilT Pseudomonas aeruginosa PAK

70.349

99.71

0.701
  pilT Acinetobacter baylyi ADP1

70.058

99.71

0.699
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.623

97.681

0.69
  pilT Vibrio cholerae strain A1552

70.623

97.681

0.69
  pilT Legionella pneumophila strain Lp02

68.314

99.71

0.681
  pilT Legionella pneumophila strain ERS1305867

68.314

99.71

0.681
  pilT Neisseria meningitidis 8013

66.276

98.841

0.655
  pilT Neisseria gonorrhoeae MS11

65.982

98.841

0.652
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

100

0.513
  pilU Vibrio cholerae strain A1552

40.58

100

0.406
  pilU Pseudomonas stutzeri DSM 10701

39.701

97.101

0.386
  pilU Acinetobacter baylyi ADP1

38.824

98.551

0.383

Multiple sequence alignment