Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   K5706_RS02360 Genome accession   NZ_AP019849
Coordinates   490443..491483 (+) Length   346 a.a.
NCBI ID   WP_128810138.1    Uniprot ID   -
Organism   Vibrio alfacsensis strain 04Ya249     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 485443..496483
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K5706_RS02330 (VA249_03860) - 486603..487205 (-) 603 WP_221068150.1 XTP/dITP diphosphatase -
  K5706_RS02335 (VA249_03870) - 487300..487731 (-) 432 WP_211915945.1 DUF4426 domain-containing protein -
  K5706_RS02340 (VA249_03880) yggU 487795..488085 (-) 291 WP_128810134.1 DUF167 family protein YggU -
  K5706_RS02345 (VA249_03890) - 488085..488642 (-) 558 WP_128810135.1 YggT family protein -
  K5706_RS02350 (VA249_03900) proC 488695..489513 (-) 819 WP_221070898.1 pyrroline-5-carboxylate reductase -
  K5706_RS02355 (VA249_03910) - 489704..490414 (-) 711 WP_221068146.1 YggS family pyridoxal phosphate-dependent enzyme -
  K5706_RS02360 (VA249_03920) pilT 490443..491483 (+) 1041 WP_128810138.1 type IV pilus twitching motility protein PilT Machinery gene
  K5706_RS02365 (VA249_03930) pilU 491511..492617 (+) 1107 WP_211915949.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K5706_RS02370 (VA249_03940) ruvX 492707..493132 (-) 426 WP_128810140.1 Holliday junction resolvase RuvX -
  K5706_RS02375 (VA249_03950) - 493163..493726 (-) 564 WP_221068143.1 YqgE/AlgH family protein -
  K5706_RS02380 (VA249_03960) gshB 493866..494816 (-) 951 WP_221068142.1 glutathione synthase -
  K5706_RS02385 (VA249_03970) rsmE 494831..495562 (-) 732 WP_128812237.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  K5706_RS02390 (VA249_03980) endA 495698..496393 (-) 696 WP_221070900.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38504.97 Da        Isoelectric Point: 6.5577

>NTDB_id=74384 K5706_RS02360 WP_128810138.1 490443..491483(+) (pilT) [Vibrio alfacsensis strain 04Ya249]
MDITELLDFSVKHNASDLHLSAGVPPMVRIDGDVRKLGIPAFTHQEVHRLVFEIMNDAQRSEFEEKLEVDFSFEMHNVGR
FRVNAFNQSRGCAAVFRTIPSSIPTLDELEAPEIFKKIANAEKGLVLVTGPTGSGKSTTLAAIVDYINRNHNKHILTIED
PIEFVHTNNKCLINQREVHRDTHSFQNALRSALREDPDVILVGEMRDKETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRNGGGRIACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
RQLMAQGMVSHDEAEKKVGLEAQQFS

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=74384 K5706_RS02360 WP_128810138.1 490443..491483(+) (pilT) [Vibrio alfacsensis strain 04Ya249]
ATGGATATCACTGAGTTACTGGATTTTAGTGTAAAACATAATGCATCAGATCTACATCTTTCTGCCGGTGTTCCCCCAAT
GGTACGTATTGATGGGGACGTAAGAAAGCTTGGCATACCAGCGTTTACACACCAAGAAGTGCATCGTCTCGTTTTTGAAA
TCATGAACGATGCACAACGCAGTGAATTCGAAGAAAAACTCGAAGTCGATTTTTCCTTTGAAATGCATAATGTCGGTCGT
TTCCGTGTGAACGCTTTTAATCAATCTCGAGGTTGTGCTGCTGTCTTTCGTACCATCCCAAGTAGCATTCCAACCTTGGA
TGAGTTAGAAGCGCCAGAAATTTTCAAAAAAATAGCGAATGCTGAAAAAGGTTTGGTGTTGGTGACAGGGCCAACGGGTT
CGGGTAAATCGACAACACTCGCTGCTATTGTTGACTACATTAACCGCAATCACAACAAGCATATCTTAACAATTGAAGAT
CCGATTGAGTTTGTTCATACCAATAACAAATGTTTGATCAACCAACGTGAAGTTCACCGTGATACCCACAGTTTCCAAAA
TGCACTGCGTAGCGCGCTCCGTGAAGACCCGGACGTGATTTTGGTCGGGGAAATGCGTGATAAGGAAACCATCAGCCTAG
CACTTACTGCCGCAGAAACCGGCCACCTTGTTTTTGGTACGCTGCATACTAGTTCAGCGGCGAAAACTATCGACCGTATT
ATTGACGTATTCCCAGGCAGCGATAAAGACATGGTACGCTCAATGCTGTCTGAATCACTGCGCTCTGTTATTGCCCAAAA
GCTATTGAAGCGAAATGGTGGCGGCCGGATTGCCTGTCATGAAATCATGATGGCAACCCCTGCTATTCGTAACCTTATTC
GCGAAGATAAAGTTGCTCAGATGTACTCTATTATTCAAACGGGTGCCGCTCATGGAATGCAAACCATGGAACAAAATGCG
CGTCAGTTGATGGCTCAAGGTATGGTTTCTCATGATGAGGCCGAAAAAAAGGTTGGCCTTGAAGCACAACAATTCTCGTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

85.797

99.711

0.855
  pilT Vibrio cholerae strain A1552

85.797

99.711

0.855
  pilT Acinetobacter baumannii D1279779

71.856

96.532

0.694
  pilT Acinetobacter baumannii strain A118

71.856

96.532

0.694
  pilT Acinetobacter nosocomialis M2

71.557

96.532

0.691
  pilT Acinetobacter baylyi ADP1

71.212

95.376

0.679
  pilT Pseudomonas stutzeri DSM 10701

69.436

97.399

0.676
  pilT Pseudomonas aeruginosa PAK

69.139

97.399

0.673
  pilT Legionella pneumophila strain Lp02

67.665

96.532

0.653
  pilT Legionella pneumophila strain ERS1305867

67.665

96.532

0.653
  pilT Neisseria meningitidis 8013

64.201

97.688

0.627
  pilT Neisseria gonorrhoeae MS11

63.905

97.688

0.624
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.28

100

0.494
  pilU Vibrio cholerae strain A1552

41.791

96.821

0.405
  pilU Pseudomonas stutzeri DSM 10701

40.597

96.821

0.393
  pilU Acinetobacter baylyi ADP1

38.757

97.688

0.379

Multiple sequence alignment