Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   K5706_RS02365 Genome accession   NZ_AP019849
Coordinates   491511..492617 (+) Length   368 a.a.
NCBI ID   WP_211915949.1    Uniprot ID   -
Organism   Vibrio alfacsensis strain 04Ya249     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 486511..497617
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K5706_RS02330 (VA249_03860) - 486603..487205 (-) 603 WP_221068150.1 XTP/dITP diphosphatase -
  K5706_RS02335 (VA249_03870) - 487300..487731 (-) 432 WP_211915945.1 DUF4426 domain-containing protein -
  K5706_RS02340 (VA249_03880) yggU 487795..488085 (-) 291 WP_128810134.1 DUF167 family protein YggU -
  K5706_RS02345 (VA249_03890) - 488085..488642 (-) 558 WP_128810135.1 YggT family protein -
  K5706_RS02350 (VA249_03900) proC 488695..489513 (-) 819 WP_221070898.1 pyrroline-5-carboxylate reductase -
  K5706_RS02355 (VA249_03910) - 489704..490414 (-) 711 WP_221068146.1 YggS family pyridoxal phosphate-dependent enzyme -
  K5706_RS02360 (VA249_03920) pilT 490443..491483 (+) 1041 WP_128810138.1 type IV pilus twitching motility protein PilT Machinery gene
  K5706_RS02365 (VA249_03930) pilU 491511..492617 (+) 1107 WP_211915949.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K5706_RS02370 (VA249_03940) ruvX 492707..493132 (-) 426 WP_128810140.1 Holliday junction resolvase RuvX -
  K5706_RS02375 (VA249_03950) - 493163..493726 (-) 564 WP_221068143.1 YqgE/AlgH family protein -
  K5706_RS02380 (VA249_03960) gshB 493866..494816 (-) 951 WP_221068142.1 glutathione synthase -
  K5706_RS02385 (VA249_03970) rsmE 494831..495562 (-) 732 WP_128812237.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  K5706_RS02390 (VA249_03980) endA 495698..496393 (-) 696 WP_221070900.1 deoxyribonuclease I -
  K5706_RS02395 (VA249_03990) - 496667..497164 (-) 498 WP_221070901.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41390.39 Da        Isoelectric Point: 6.5076

>NTDB_id=74385 K5706_RS02365 WP_211915949.1 491511..492617(+) (pilU) [Vibrio alfacsensis strain 04Ya249]
MDLNKFLEGMLTLKASDLYITVGAPILFRVDGELRPQGEKLTEHDVAQLLDSAMEPDRRREFRSSRESNFAIVRDCGRFR
VSAFFQRELPGAVIRRIETNIPTFEQLKLPLVLQDLAVAKRGLVLVVGATGSGKSTTMAAMTGYRNNNKAGHILTVEDPI
EFVHEHKRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPNDQKEQFLFDLSMNLKGVIGQQLIRDKNGQGRHGVFEVLLNSPRVSDLIRRGDLHELKATMARSNEFGMLTFDQSLY
KLVMQGKISEEDALHSADSANDLRLMLKTQRGESFSSGSLENVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=74385 K5706_RS02365 WP_211915949.1 491511..492617(+) (pilU) [Vibrio alfacsensis strain 04Ya249]
ATGGATCTGAACAAATTTCTTGAGGGTATGCTCACGCTTAAAGCGTCGGACCTCTATATTACGGTCGGTGCTCCAATACT
GTTTCGAGTTGATGGAGAGCTCCGCCCACAAGGTGAGAAGCTGACCGAGCATGATGTAGCGCAGTTGCTTGATAGTGCCA
TGGAGCCCGATCGTCGCCGAGAATTTCGTAGTAGCCGTGAATCTAACTTCGCCATAGTAAGAGACTGTGGGCGTTTTCGC
GTCAGCGCTTTTTTTCAGCGTGAATTACCAGGGGCGGTGATTCGTCGTATTGAGACCAATATTCCGACCTTTGAGCAATT
AAAGTTGCCGTTGGTTTTGCAAGATCTTGCTGTTGCTAAGCGAGGGCTAGTGCTGGTGGTTGGTGCGACAGGGTCGGGTA
AATCGACGACAATGGCTGCAATGACGGGGTATCGAAACAACAATAAAGCGGGTCATATTCTAACGGTTGAGGATCCCATT
GAGTTTGTGCATGAGCATAAGCGTTGTATCGTCACACAACGAGAAGTGGGTTTAGATACTGAAAGCTACGAGGTGGCGCT
TAAGAACTCGCTCCGTCAAGCGCCAGACATGATTTTGATTGGTGAAATTCGCAGTCGAGAAACGATGGAATATGCGATGA
CGTTCGCGGAAACTGGGCATCTTTGCATGGCAACCCTGCACGCTAACAATGCTAACCAAGCCCTTGAGCGCATCTTACAT
TTAGTGCCAAACGATCAAAAAGAGCAGTTTCTATTTGATTTGTCGATGAACTTAAAAGGTGTGATAGGCCAACAACTTAT
CAGAGATAAAAATGGTCAAGGTCGACATGGGGTATTTGAAGTTCTGTTAAATAGCCCGCGAGTTTCTGATTTAATTCGTC
GAGGTGATTTACATGAACTCAAAGCGACGATGGCACGCTCAAATGAATTTGGTATGTTGACGTTCGACCAGTCACTCTAC
AAGTTGGTGATGCAAGGTAAGATCAGCGAGGAGGATGCGTTGCACAGTGCGGATTCTGCAAATGATCTTCGCTTAATGTT
GAAAACTCAGCGCGGAGAATCTTTCTCATCGGGCAGCTTAGAAAACGTTAAGATCGATATGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(27-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

82.88

100

0.829
  pilU Pseudomonas stutzeri DSM 10701

58.857

95.109

0.56
  pilU Acinetobacter baylyi ADP1

55.556

95.38

0.53
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.363

92.12

0.399
  pilT Acinetobacter baumannii strain A118

42.183

92.12

0.389
  pilT Acinetobacter baumannii D1279779

42.183

92.12

0.389
  pilT Acinetobacter nosocomialis M2

42.183

92.12

0.389
  pilT Pseudomonas aeruginosa PAK

41.888

92.12

0.386
  pilT Acinetobacter baylyi ADP1

41.642

92.663

0.386
  pilT Legionella pneumophila strain Lp02

41.593

92.12

0.383
  pilT Legionella pneumophila strain ERS1305867

41.593

92.12

0.383
  pilT Pseudomonas stutzeri DSM 10701

40.708

92.12

0.375
  pilT Vibrio cholerae strain A1552

40.719

90.761

0.37
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.719

90.761

0.37

Multiple sequence alignment