Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GL2_RS05675 Genome accession   NZ_AP019807
Coordinates   1323243..1324277 (+) Length   344 a.a.
NCBI ID   WP_143729687.1    Uniprot ID   A0A510IQP9
Organism   Microbulbifer sp. GL-2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1318243..1329277
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GL2_RS05650 (GL2_11470) - 1318426..1319034 (-) 609 WP_143729682.1 YggT family protein -
  GL2_RS05655 (GL2_11480) proC 1319097..1319930 (-) 834 WP_143729683.1 pyrroline-5-carboxylate reductase -
  GL2_RS05660 (GL2_11490) - 1320073..1322076 (+) 2004 WP_143729684.1 prolyl oligopeptidase family serine peptidase -
  GL2_RS05665 - 1322154..1322429 (-) 276 WP_143729685.1 hypothetical protein -
  GL2_RS05670 (GL2_11510) - 1322469..1323173 (-) 705 WP_143729686.1 YggS family pyridoxal phosphate-dependent enzyme -
  GL2_RS05675 (GL2_11520) pilT 1323243..1324277 (+) 1035 WP_143729687.1 type IV pilus twitching motility protein PilT Machinery gene
  GL2_RS05680 (GL2_11530) pilU 1324343..1325494 (+) 1152 WP_143729688.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GL2_RS05685 (GL2_11540) - 1325645..1326400 (+) 756 WP_143729689.1 SDR family oxidoreductase -
  GL2_RS05690 (GL2_11550) - 1326412..1327011 (+) 600 WP_143729690.1 LysE family translocator -
  GL2_RS05695 (GL2_11560) - 1327024..1327770 (-) 747 WP_143729691.1 LytTR family DNA-binding domain-containing protein -
  GL2_RS05700 (GL2_11570) - 1327767..1328924 (-) 1158 WP_143729692.1 sensor histidine kinase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38331.01 Da        Isoelectric Point: 6.5786

>NTDB_id=74214 GL2_RS05675 WP_143729687.1 1323243..1324277(+) (pilT) [Microbulbifer sp. GL-2]
MDITELLAFSAKQNASDLHLSAGLPPMIRVDGDVRRINLPSMEHKQVHSLIYEIMNDKQRKDFEELLETDFSFEVPGVAR
FRVNAFNHNRGAGAVFRTIPSKVLTMEDLGMGQVFKQISDTPRGLVLVTGPTGSGKSTTLAAMMDYINDNKYEHILTIED
PIEFVHESKKCLVNQREVHRDTHGFAEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAQEKAMVRSMLSESLQAVVSQTLMKKNGGGRVAAHEIMRGTPAIRNLIREDKIAQMYSAIQTGASVGMQTMDQCL
EDLVARRIISREVAREKAKMPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=74214 GL2_RS05675 WP_143729687.1 1323243..1324277(+) (pilT) [Microbulbifer sp. GL-2]
ATGGATATCACTGAACTCCTCGCCTTTAGCGCCAAGCAAAACGCCTCGGATTTGCATCTCTCGGCCGGCCTACCGCCGAT
GATTCGGGTGGACGGCGACGTACGCCGCATCAACCTGCCGTCCATGGAGCATAAGCAGGTGCACAGCCTGATCTACGAGA
TCATGAACGACAAGCAGCGCAAAGATTTCGAAGAGCTGCTGGAAACGGATTTCTCATTCGAAGTGCCCGGTGTAGCCCGT
TTCCGTGTCAATGCGTTTAACCACAACCGCGGTGCCGGTGCTGTATTCCGGACTATTCCCTCCAAGGTACTGACCATGGA
AGATCTGGGTATGGGCCAGGTCTTCAAACAAATATCGGATACCCCTCGCGGTCTGGTATTAGTGACCGGGCCCACAGGTT
CCGGTAAGTCCACTACGCTGGCTGCGATGATGGACTACATCAATGACAACAAATACGAGCATATTCTCACCATTGAAGAC
CCGATTGAATTCGTGCATGAGTCGAAGAAATGTCTGGTGAACCAGCGTGAGGTACATCGTGATACCCACGGCTTTGCCGA
GGCACTGCGCTCAGCACTGCGTGAAGACCCGGATATTATCCTGGTGGGTGAAATGCGTGATTTGGAGACCATCAGGTTGG
CACTGACCGCTGCGGAAACCGGCCACCTGGTGTTTGGTACCCTGCACACCACCTCCGCTGCCAAGACCATCGACCGGGTT
GTGGACGTATTCCCGGCACAGGAAAAAGCGATGGTGCGTTCCATGCTGTCGGAATCCCTGCAAGCCGTAGTCTCCCAGAC
CCTGATGAAGAAGAACGGCGGTGGCCGTGTCGCCGCCCACGAAATTATGCGTGGCACCCCAGCGATCCGTAACCTGATCC
GCGAAGACAAAATCGCGCAAATGTACTCGGCTATCCAGACTGGTGCCAGCGTAGGCATGCAGACCATGGACCAATGCCTG
GAAGACCTGGTGGCTCGCCGTATCATCAGCCGCGAAGTCGCGCGTGAAAAAGCCAAGATGCCGGATAACTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A510IQP9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

88.953

100

0.89
  pilT Pseudomonas stutzeri DSM 10701

87.5

100

0.875
  pilT Acinetobacter baumannii D1279779

83.43

100

0.834
  pilT Acinetobacter baumannii strain A118

83.43

100

0.834
  pilT Acinetobacter nosocomialis M2

83.14

100

0.831
  pilT Acinetobacter baylyi ADP1

79.36

100

0.794
  pilT Legionella pneumophila strain Lp02

74.556

98.256

0.733
  pilT Legionella pneumophila strain ERS1305867

74.556

98.256

0.733
  pilT Neisseria meningitidis 8013

67.941

98.837

0.672
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.647

98.837

0.669
  pilT Vibrio cholerae strain A1552

67.647

98.837

0.669
  pilT Neisseria gonorrhoeae MS11

67.353

98.837

0.666
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

100

0.517
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.384

0.398
  pilU Vibrio cholerae strain A1552

39.42

100

0.395
  pilU Acinetobacter baylyi ADP1

38.728

100

0.39

Multiple sequence alignment