Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   GL2_RS05680 Genome accession   NZ_AP019807
Coordinates   1324343..1325494 (+) Length   383 a.a.
NCBI ID   WP_143729688.1    Uniprot ID   A0A510IQQ9
Organism   Microbulbifer sp. GL-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1319343..1330494
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GL2_RS05660 (GL2_11490) - 1320073..1322076 (+) 2004 WP_143729684.1 prolyl oligopeptidase family serine peptidase -
  GL2_RS05665 - 1322154..1322429 (-) 276 WP_143729685.1 hypothetical protein -
  GL2_RS05670 (GL2_11510) - 1322469..1323173 (-) 705 WP_143729686.1 YggS family pyridoxal phosphate-dependent enzyme -
  GL2_RS05675 (GL2_11520) pilT 1323243..1324277 (+) 1035 WP_143729687.1 type IV pilus twitching motility protein PilT Machinery gene
  GL2_RS05680 (GL2_11530) pilU 1324343..1325494 (+) 1152 WP_143729688.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GL2_RS05685 (GL2_11540) - 1325645..1326400 (+) 756 WP_143729689.1 SDR family oxidoreductase -
  GL2_RS05690 (GL2_11550) - 1326412..1327011 (+) 600 WP_143729690.1 LysE family translocator -
  GL2_RS05695 (GL2_11560) - 1327024..1327770 (-) 747 WP_143729691.1 LytTR family DNA-binding domain-containing protein -
  GL2_RS05700 (GL2_11570) - 1327767..1328924 (-) 1158 WP_143729692.1 sensor histidine kinase -
  GL2_RS05705 (GL2_11580) argH 1329014..1330435 (+) 1422 WP_143729693.1 argininosuccinate lyase -

Sequence

Protein


Download         Length: 383 a.a.        Molecular weight: 42727.98 Da        Isoelectric Point: 6.2374

>NTDB_id=74215 GL2_RS05680 WP_143729688.1 1324343..1325494(+) (pilU) [Microbulbifer sp. GL-2]
MEIERLLQLVVEKGASDLFITAGIPPSLKLHGRVVPCTSTSLTPEKARELVVGTMNEKQRREFAEQKELNYAIAVRNVGR
FRVSAFFQRNLVGMVLRRIETKIPTIDGLSLPDVLKDLAMTKRGLIIFVGATGTGKSTSLASMVGHRNENSKGHIITIED
PIEFIHQHRGCIVTQREVGLDTESFEVALKNTLRQAPDVILIGEVRSRETMDHAIAFAETGHLCLCTLHANNANQALDRI
IHFFPADRHQQLFMDLSLNLKAMVAQQLVPTPDGDGRRACLEIMINTPLMSDLIRKGEVHKMKELMKRSGEQGMQTFDQA
LYELYDRGEITYEDALAHADSSNDLRLMIKLKSESDAEYLNSAAGELSLQNDSEYGDGGPQLY

Nucleotide


Download         Length: 1152 bp        

>NTDB_id=74215 GL2_RS05680 WP_143729688.1 1324343..1325494(+) (pilU) [Microbulbifer sp. GL-2]
ATGGAAATTGAAAGACTTCTACAACTGGTTGTAGAGAAAGGCGCTTCGGATCTGTTTATCACCGCCGGCATACCCCCCTC
CTTAAAGCTGCACGGCCGAGTTGTGCCCTGCACAAGTACTTCACTCACACCAGAGAAGGCGCGCGAACTGGTGGTAGGTA
CCATGAACGAAAAACAGCGACGTGAATTCGCTGAACAGAAGGAACTAAACTACGCCATCGCCGTGCGCAACGTGGGACGC
TTCCGGGTCTCAGCTTTTTTCCAACGCAATCTGGTGGGTATGGTACTACGTCGTATCGAGACCAAGATCCCTACCATTGA
TGGTCTCAGCCTGCCCGATGTGCTCAAAGACTTGGCGATGACCAAGCGCGGCCTGATTATTTTCGTCGGCGCCACCGGTA
CCGGTAAATCCACATCCCTCGCATCCATGGTCGGCCATCGCAATGAGAACTCCAAAGGCCATATCATCACTATTGAGGAC
CCTATCGAATTTATCCACCAACACCGCGGCTGTATTGTGACCCAGCGTGAGGTTGGCCTGGATACCGAATCCTTCGAGGT
GGCCCTGAAGAACACCCTGCGACAGGCGCCAGACGTTATCCTGATTGGTGAGGTGCGCTCCCGCGAAACCATGGACCACG
CCATCGCCTTTGCCGAAACCGGTCACTTGTGCCTGTGCACGCTGCACGCCAACAACGCTAACCAGGCGTTAGATCGTATC
ATCCACTTCTTCCCGGCGGACCGCCATCAGCAGCTGTTTATGGACCTGTCGTTGAACCTTAAGGCGATGGTAGCTCAGCA
GTTGGTACCCACTCCGGACGGCGACGGCCGCCGCGCCTGTCTGGAAATCATGATCAATACCCCGCTGATGTCGGACCTGA
TCCGCAAGGGCGAGGTACACAAAATGAAAGAATTAATGAAGCGCTCCGGCGAGCAGGGTATGCAGACCTTCGATCAGGCC
CTGTATGAACTCTACGACCGCGGCGAGATCACCTATGAAGATGCCCTCGCCCACGCCGACTCCTCCAATGACCTGCGCCT
GATGATCAAACTCAAATCTGAATCAGATGCCGAATATTTAAACAGTGCAGCAGGGGAATTGAGCCTGCAAAACGACTCCG
AATATGGTGATGGTGGGCCGCAACTGTATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A510IQQ9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

75.676

96.606

0.731
  pilU Acinetobacter baylyi ADP1

67.227

93.211

0.627
  pilU Vibrio cholerae strain A1552

54.987

96.867

0.533
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.838

88.512

0.397
  pilT Acinetobacter nosocomialis M2

42.388

87.467

0.371
  pilT Acinetobacter baumannii D1279779

42.388

87.467

0.371
  pilT Acinetobacter baumannii strain A118

42.388

87.467

0.371
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.617

87.206

0.363
  pilT Vibrio cholerae strain A1552

41.617

87.206

0.363
  pilT Pseudomonas aeruginosa PAK

41.194

87.467

0.36
  pilT Acinetobacter baylyi ADP1

40.469

89.034

0.36

Multiple sequence alignment