Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RxyAA322_RS02375 Genome accession   NZ_AP019791
Coordinates   452995..454092 (-) Length   365 a.a.
NCBI ID   WP_143526743.1    Uniprot ID   A0A510HF90
Organism   Rubrobacter xylanophilus strain AA3-22     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 447995..459092
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RxyAA322_RS02350 (RxyAA322_04690) - 449424..449996 (-) 573 WP_143526738.1 hypothetical protein -
  RxyAA322_RS02355 (RxyAA322_04700) - 449993..450418 (-) 426 WP_172620625.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  RxyAA322_RS02360 (RxyAA322_04710) - 450411..450986 (-) 576 WP_143526740.1 GspH/FimT family pseudopilin -
  RxyAA322_RS02365 (RxyAA322_04720) - 450999..451769 (-) 771 WP_143526741.1 sigma-70 family RNA polymerase sigma factor -
  RxyAA322_RS02370 (RxyAA322_04730) pilC 451784..452995 (-) 1212 WP_143526742.1 type II secretion system F family protein Machinery gene
  RxyAA322_RS02375 (RxyAA322_04740) pilT 452995..454092 (-) 1098 WP_143526743.1 type IV pilus twitching motility protein PilT Machinery gene
  RxyAA322_RS02380 (RxyAA322_04750) - 454089..455798 (-) 1710 WP_143526744.1 ATPase, T2SS/T4P/T4SS family -
  RxyAA322_RS02385 (RxyAA322_04760) - 456050..457165 (+) 1116 WP_143526745.1 type IV pilus twitching motility protein PilT -
  RxyAA322_RS02390 (RxyAA322_04770) - 457251..458351 (+) 1101 WP_143526746.1 aminopeptidase -
  RxyAA322_RS02395 (RxyAA322_04780) - 458356..458595 (+) 240 WP_143526747.1 hypothetical protein -

Sequence

Protein


Download         Length: 365 a.a.        Molecular weight: 39654.09 Da        Isoelectric Point: 6.8891

>NTDB_id=74022 RxyAA322_RS02375 WP_143526743.1 452995..454092(-) (pilT) [Rubrobacter xylanophilus strain AA3-22]
MSGAVAENGLADFLLEMLEAGASDLHLTAGSPPVVRVDGEVRPLDHPPLTPATTRDLVYDILTDEQRRRLENDWELDFSY
SVPGAARFRVNVYFQRGSLGAAFRAVPNEIKSLNELGLPPVVESLTEKPRGLVLVTGPTGSGKSTTLAAMIDRINETRSG
HIMSIEDPIEFLHRHKRCIVNQREVASDTRSFAAALRHVLRQDPDVILVGEMRDLETISLAVTAAETGHLVFGTLHTQDA
PQTVDRIVDVFPPHQQGQVRAQLANALQGIVTQTLLKRREGRGRVVACEVLVATPGVRNLIREGKNHQLYSAMQTGGKFG
MQTLDAALASLVRRGLVSPEEAGRHAANPDELRRLGGTGGARGAF

Nucleotide


Download         Length: 1098 bp        

>NTDB_id=74022 RxyAA322_RS02375 WP_143526743.1 452995..454092(-) (pilT) [Rubrobacter xylanophilus strain AA3-22]
ATGAGCGGCGCGGTGGCGGAGAACGGGCTCGCGGACTTCCTGCTCGAGATGCTCGAGGCGGGGGCCAGCGACCTGCACCT
CACGGCGGGAAGTCCGCCGGTGGTCCGGGTGGACGGCGAGGTGCGACCGCTGGACCACCCCCCGCTCACCCCGGCAACCA
CCCGCGACCTCGTCTACGACATCCTCACCGACGAGCAGCGGCGGCGCCTGGAGAACGACTGGGAGCTGGACTTCTCCTAC
TCGGTCCCCGGCGCGGCCCGCTTCCGGGTCAACGTCTATTTCCAGCGGGGGTCGCTCGGGGCGGCCTTCCGGGCCGTCCC
CAACGAGATAAAGAGCCTGAACGAGCTGGGGCTGCCGCCAGTCGTGGAGTCCCTCACCGAGAAGCCGCGCGGGCTCGTAC
TCGTCACCGGGCCCACCGGCAGCGGCAAGTCCACCACCCTCGCCGCCATGATCGACCGGATAAACGAGACCCGCAGCGGC
CACATCATGAGCATCGAGGACCCCATAGAGTTTTTGCACCGGCACAAGCGGTGCATCGTCAACCAGCGGGAGGTCGCCTC
GGACACCCGCAGCTTCGCCGCGGCGCTCCGGCACGTGCTGCGACAGGACCCGGACGTGATCCTGGTGGGCGAGATGCGGG
ATCTGGAGACCATCTCGCTCGCGGTGACCGCCGCGGAGACCGGGCACCTGGTCTTCGGGACGCTGCACACCCAGGACGCC
CCGCAGACCGTGGACCGGATCGTGGACGTCTTCCCGCCCCACCAGCAGGGCCAGGTGCGCGCGCAGCTGGCCAACGCGCT
GCAGGGCATCGTCACCCAGACGCTCCTGAAGCGGCGCGAGGGGCGCGGGAGGGTGGTGGCCTGCGAGGTGCTGGTGGCCA
CCCCGGGGGTCCGCAACCTCATCCGGGAGGGCAAGAACCACCAGCTCTACTCGGCCATGCAGACCGGTGGGAAGTTCGGG
ATGCAGACGCTGGACGCCGCGCTGGCCAGCCTCGTGCGCCGGGGGCTCGTCTCCCCCGAGGAGGCCGGGCGGCACGCGGC
GAACCCCGACGAGCTGCGGCGGCTCGGCGGGACGGGCGGCGCGCGGGGGGCCTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-263)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A510HF90

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

54.342

97.808

0.532
  pilT Pseudomonas aeruginosa PAK

57.53

90.959

0.523
  pilT Acinetobacter nosocomialis M2

55.102

93.973

0.518
  pilT Acinetobacter baumannii strain A118

55.102

93.973

0.518
  pilT Acinetobacter baumannii D1279779

55.102

93.973

0.518
  pilT Pseudomonas stutzeri DSM 10701

56.024

90.959

0.51
  pilT Acinetobacter baylyi ADP1

55.891

90.685

0.507
  pilT Legionella pneumophila strain ERS1305867

53.687

92.877

0.499
  pilT Legionella pneumophila strain Lp02

53.687

92.877

0.499
  pilT Neisseria meningitidis 8013

52.478

93.973

0.493
  pilT Neisseria gonorrhoeae MS11

52.187

93.973

0.49
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.108

90.959

0.474
  pilT Vibrio cholerae strain A1552

52.108

90.959

0.474
  pilU Pseudomonas stutzeri DSM 10701

45.994

92.329

0.425
  pilU Vibrio cholerae strain A1552

43.605

94.247

0.411
  pilU Acinetobacter baylyi ADP1

40.233

93.973

0.378

Multiple sequence alignment