Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   K5658_RS16265 Genome accession   NZ_AP019783
Coordinates   3570518..3571552 (+) Length   344 a.a.
NCBI ID   WP_221064150.1    Uniprot ID   -
Organism   Methylomagnum ishizawai strain RS11D-Pr     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3565518..3576552
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K5658_RS16240 (MishRS11D_32170) - 3565904..3567889 (-) 1986 WP_221064145.1 dynamin family protein -
  K5658_RS16245 (MishRS11D_32180) - 3567969..3568268 (-) 300 WP_221064146.1 DUF167 family protein -
  K5658_RS16250 (MishRS11D_32190) - 3568270..3568845 (-) 576 WP_221064147.1 YggT family protein -
  K5658_RS16255 (MishRS11D_32200) proC 3568849..3569673 (-) 825 WP_221064148.1 pyrroline-5-carboxylate reductase -
  K5658_RS16260 (MishRS11D_32210) - 3569737..3570426 (-) 690 WP_221064149.1 YggS family pyridoxal phosphate-dependent enzyme -
  K5658_RS16265 (MishRS11D_32230) pilT 3570518..3571552 (+) 1035 WP_221064150.1 type IV pilus twitching motility protein PilT Machinery gene
  K5658_RS16270 (MishRS11D_32240) pilU 3571580..3572701 (+) 1122 WP_221067000.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K5658_RS16275 (MishRS11D_32250) - 3572790..3573347 (+) 558 WP_221064151.1 DUF2726 domain-containing protein -
  K5658_RS16280 (MishRS11D_32260) - 3573355..3573696 (+) 342 WP_221064152.1 ArsC family reductase -
  K5658_RS16285 (MishRS11D_32270) dapE 3573693..3574820 (+) 1128 WP_221064153.1 succinyl-diaminopimelate desuccinylase -
  K5658_RS16290 (MishRS11D_32280) sseA 3574869..3575720 (+) 852 WP_221064154.1 3-mercaptopyruvate sulfurtransferase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38329.98 Da        Isoelectric Point: 6.8359

>NTDB_id=74014 K5658_RS16265 WP_221064150.1 3570518..3571552(+) (pilT) [Methylomagnum ishizawai strain RS11D-Pr]
MDIAELLAFSVKNNASDLHLSAGLPPMIRVDGDIRRINVPALDHKEVHALVYDIMNDKQRRDYEEFLETDFSFELPGVAR
FRVNAFNQERGAAAVFRTIPSKILTLEELSCPKFFQDVTRHPRGLIVVTGPTGSGKSTTLAAMIDYINSNDYSHILTVED
PIEFVHPSKKCLINQREVHRDTLGFNEALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAAEKGIVRSMLSESLQAVITQALLKKNGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSTIQTSRKDGMQTLDQHL
LELVEKGLISRQIARTKAVNKANF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=74014 K5658_RS16265 WP_221064150.1 3570518..3571552(+) (pilT) [Methylomagnum ishizawai strain RS11D-Pr]
ATGGATATCGCCGAACTCCTTGCCTTTTCCGTCAAGAACAACGCCTCCGACCTCCACTTGTCGGCGGGACTCCCCCCCAT
GATCCGCGTGGACGGCGATATCCGCCGCATCAACGTCCCGGCCCTGGACCACAAGGAAGTCCACGCGCTGGTCTACGACA
TCATGAACGACAAACAGCGCCGCGATTATGAAGAATTCCTGGAAACCGACTTTTCCTTCGAGCTACCCGGCGTGGCCCGT
TTCCGTGTCAACGCCTTCAACCAGGAGCGCGGTGCCGCCGCCGTGTTCCGTACCATCCCCTCGAAAATCCTGACCCTGGA
AGAACTGAGCTGCCCCAAATTCTTCCAGGACGTGACCCGCCATCCGCGCGGCCTGATCGTCGTGACCGGCCCCACCGGCT
CGGGCAAATCCACCACCCTGGCCGCCATGATCGACTACATCAATTCCAACGACTATTCCCACATCCTGACCGTCGAAGAC
CCCATCGAATTCGTGCATCCCAGCAAGAAATGCCTTATCAACCAACGCGAGGTCCACCGCGATACCCTGGGCTTCAACGA
AGCCTTGCGCTCGGCCCTGCGCGAAGACCCCGACGTGATCCTGGTCGGCGAAATGCGCGACCTCGAAACCATCCGTCTGG
CCCTGACCGCCGCCGAAACCGGGCATTTGGTGTTCGGCACCCTGCACACCAGTTCCGCCGCCAAGACCATCGACCGTATC
ATCGACGTGTTCCCCGCCGCCGAGAAAGGCATCGTCCGTTCCATGCTGTCGGAATCGCTGCAAGCCGTCATCACCCAGGC
GCTCCTCAAAAAGAACGGCGGTGGCCGCACGGCGGCCTGGGAAATCATGGTCGGCACCCCCGCCATCCGCAACCTGATCC
GCGAGGACAAAGTGGCCCAGATGTATTCCACCATCCAAACCAGCCGCAAGGACGGGATGCAAACCCTGGATCAACACCTG
CTGGAACTGGTGGAAAAAGGCCTCATCTCGCGCCAGATCGCCCGCACCAAAGCCGTCAACAAAGCCAATTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.651

100

0.797
  pilT Acinetobacter baumannii D1279779

79.651

100

0.797
  pilT Acinetobacter baumannii strain A118

79.651

100

0.797
  pilT Pseudomonas aeruginosa PAK

79.36

100

0.794
  pilT Pseudomonas stutzeri DSM 10701

79.07

100

0.791
  pilT Acinetobacter baylyi ADP1

78.198

100

0.782
  pilT Legionella pneumophila strain Lp02

75

100

0.75
  pilT Legionella pneumophila strain ERS1305867

75

100

0.75
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.436

97.965

0.68
  pilT Vibrio cholerae strain A1552

69.436

97.965

0.68
  pilT Neisseria meningitidis 8013

67.155

99.128

0.666
  pilT Neisseria gonorrhoeae MS11

66.862

99.128

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.435

100

0.506
  pilU Vibrio cholerae strain A1552

42.09

97.384

0.41
  pilU Pseudomonas stutzeri DSM 10701

41.194

97.384

0.401
  pilU Acinetobacter baylyi ADP1

40.06

96.512

0.387

Multiple sequence alignment