Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   K5658_RS16270 Genome accession   NZ_AP019783
Coordinates   3571580..3572701 (+) Length   373 a.a.
NCBI ID   WP_221067000.1    Uniprot ID   -
Organism   Methylomagnum ishizawai strain RS11D-Pr     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3566580..3577701
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K5658_RS16245 (MishRS11D_32180) - 3567969..3568268 (-) 300 WP_221064146.1 DUF167 family protein -
  K5658_RS16250 (MishRS11D_32190) - 3568270..3568845 (-) 576 WP_221064147.1 YggT family protein -
  K5658_RS16255 (MishRS11D_32200) proC 3568849..3569673 (-) 825 WP_221064148.1 pyrroline-5-carboxylate reductase -
  K5658_RS16260 (MishRS11D_32210) - 3569737..3570426 (-) 690 WP_221064149.1 YggS family pyridoxal phosphate-dependent enzyme -
  K5658_RS16265 (MishRS11D_32230) pilT 3570518..3571552 (+) 1035 WP_221064150.1 type IV pilus twitching motility protein PilT Machinery gene
  K5658_RS16270 (MishRS11D_32240) pilU 3571580..3572701 (+) 1122 WP_221067000.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K5658_RS16275 (MishRS11D_32250) - 3572790..3573347 (+) 558 WP_221064151.1 DUF2726 domain-containing protein -
  K5658_RS16280 (MishRS11D_32260) - 3573355..3573696 (+) 342 WP_221064152.1 ArsC family reductase -
  K5658_RS16285 (MishRS11D_32270) dapE 3573693..3574820 (+) 1128 WP_221064153.1 succinyl-diaminopimelate desuccinylase -
  K5658_RS16290 (MishRS11D_32280) sseA 3574869..3575720 (+) 852 WP_221064154.1 3-mercaptopyruvate sulfurtransferase -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 42089.34 Da        Isoelectric Point: 6.5636

>NTDB_id=74015 K5658_RS16270 WP_221067000.1 3571580..3572701(+) (pilU) [Methylomagnum ishizawai strain RS11D-Pr]
MDFDTLLMLMTHKKASDLFITTNKEPCLKIDGQLQPISNAKLSKDQAYQLVLSVMNQRQRDEFENTKECNFAISAEGLGR
FRVSAFMQRDAAGMVLRRIETHIPTVEDLHLPPVLNELAMTKRGLILFVGATGTGKSTSLAALIKYRNENSTGHIITIED
PLEFVHPHAGCIVTQREVGLDTESYEVALKNTLRQAPDVILIGEIRTRETMQHAIAFAETGHLCLSTLHANNANQALDRI
LHFFPEDMYSQLYMDLSMNLKAVIAQQLIRRADGKGRYPAIEILINTPLAADHIRKGEIHKLKELMKQSRQHGMQTFDQC
LYDLYKSGKITYEDALNSADSKNEVRLMIKLGEEGSVDKYAPRSDKIFIVDDD

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=74015 K5658_RS16270 WP_221067000.1 3571580..3572701(+) (pilU) [Methylomagnum ishizawai strain RS11D-Pr]
ATGGATTTCGATACCCTGCTGATGCTGATGACGCACAAGAAAGCGTCGGACCTCTTCATCACCACCAACAAGGAACCCTG
CCTCAAGATCGACGGCCAGCTACAACCGATCTCCAACGCCAAACTATCCAAGGACCAGGCCTACCAACTGGTGCTGAGCG
TGATGAACCAACGCCAGCGCGACGAGTTCGAGAACACCAAGGAATGCAACTTCGCGATCTCGGCGGAAGGGCTGGGCCGG
TTCCGGGTCAGCGCCTTCATGCAGCGCGACGCCGCCGGGATGGTGCTGCGCCGGATCGAAACCCATATCCCCACGGTGGA
GGATTTGCACCTGCCGCCGGTCCTCAACGAACTGGCGATGACCAAGCGCGGCCTGATCCTGTTCGTGGGCGCGACCGGCA
CCGGCAAATCCACCTCGCTCGCCGCCCTCATCAAATACCGCAACGAGAATTCCACCGGCCATATCATCACCATCGAAGAC
CCCTTGGAATTCGTCCATCCCCATGCCGGTTGCATCGTGACCCAGCGCGAGGTCGGCCTCGACACCGAATCCTACGAAGT
CGCGCTCAAGAACACCCTCCGCCAAGCCCCCGACGTGATCCTGATCGGCGAAATCCGCACCCGCGAGACCATGCAGCACG
CCATCGCCTTCGCCGAGACCGGCCATCTCTGCCTCTCCACCCTGCACGCCAACAACGCCAACCAAGCCCTCGACCGAATT
CTGCATTTCTTCCCGGAAGACATGTATTCGCAGCTGTATATGGACTTGTCGATGAACCTCAAGGCCGTCATCGCCCAGCA
GCTCATCCGCCGCGCCGATGGCAAGGGCCGTTATCCCGCCATCGAAATCCTCATCAACACGCCCCTGGCCGCCGACCACA
TCCGCAAGGGCGAAATCCACAAGCTCAAGGAGCTGATGAAACAATCCCGCCAGCACGGGATGCAGACCTTCGACCAATGC
CTGTACGATCTCTATAAGAGCGGCAAGATCACCTACGAGGACGCACTGAATTCGGCGGACTCCAAGAACGAGGTGCGCCT
GATGATCAAACTGGGCGAGGAAGGCAGCGTGGACAAATACGCCCCGCGCAGCGACAAGATTTTCATCGTGGACGACGATT
GA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

69.209

94.906

0.657
  pilU Acinetobacter baylyi ADP1

65.266

95.71

0.625
  pilU Vibrio cholerae strain A1552

59.649

91.689

0.547
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.529

93.298

0.397
  pilT Legionella pneumophila strain Lp02

41.399

91.957

0.381
  pilT Legionella pneumophila strain ERS1305867

41.399

91.957

0.381
  pilT Pseudomonas stutzeri DSM 10701

39.823

90.885

0.362
  pilT Pseudomonas aeruginosa PAK

39.823

90.885

0.362

Multiple sequence alignment